Injectable thermosensitive chitosan hydrogels with controlled gelation kinetics and enhanced mechanical resistance

Assaad, Elias; Maire, Marion; Lerouge, Sophie

doi:10.1016/j.carbpol.2015.04.063

Cited by 126 publications

(125 citation statements)

References 22 publications

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“…Indeed, we and others have shown that the concentration of BGP required to reach rapid gelation (0.4 M) is cytotoxic to cells due to its hyperosmolality [25,26]. Culture medium incubated with CH-BGP hydrogels prepared with BGP of 0.4 M or above had a cytotoxic effect on cells [26]. Moreover, direct encapsulation of L929 mouse fibroblasts led to poor cell viability and growth (data not shown).…”

Section: Introductionmentioning

confidence: 99%

“…Though this thermogel has been considered for several biomedical applications, its mechanical properties are very poor and its biocompatibility is limited. Indeed, we and others have shown that the concentration of BGP required to reach rapid gelation (0.4 M) is cytotoxic to cells due to its hyperosmolality [25,26]. Culture medium incubated with CH-BGP hydrogels prepared with BGP of 0.4 M or above had a cytotoxic effect on cells [26].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, direct encapsulation of L929 mouse fibroblasts led to poor cell viability and growth (data not shown). These limitations were recently overcome by replacing BGP with a combination of sodium hydrogen carbonate (NaHCO 3, hereafter called SHC) and phosphate buffer (PB) [26]. This combination greatly enhanced the mechanical properties of the chitosan hydrogel, while keeping its thermosensitive properties, rapid gelation at 37 °C and low salt concentration, suggesting that it might be ideal for cell encapsulation.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, when mixed with an acidic chitosan solution the reaction between SHC (HCO 3 − ) and proton (H + ) leads to the generation of carbon dioxide (CO 2 ) [27,28]. Thus our previous work showed that the final concentration of both gelling agents influences gelation kinetic and porosity, and the modulation of their ratios allows the thermogel formulation to be adjusted according to the specific needs of individual clinical applications [26].…”

Section: Introductionmentioning

confidence: 99%

“…Based on our previous results [26], the concentration of PB was fixed to 0.04 M to reach adequate gelation kinetics and injectability, and three different concentrations of SHC were used to obtain hydrogels with different porosities influencing cell proliferation and release: SHC 0.05 M (CTGel1), SHC 0.075 M (CTGel2) and SHC 0.12 M (CTGel3) (see Table 1). …”

Section: Introductionmentioning

confidence: 99%

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Chitosan thermogels for local expansion and delivery of tumor-specific T lymphocytes towards enhanced cancer immunotherapies

et al. 2016

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Abstract:The success of promising anti-cancer adoptive cell therapies relies on the abilities of the perfused CD8 + T lymphocytes to gain access to and persist within the tumor microenvironment to carry out their cytotoxic functions. We propose a new method for their local delivery as a living concentrate, which may not only reduce the numbers of cells required for treatment but also enhance their site-specific mobilization. Using combinations of sodium hydrogen carbonate and phosphate buffer as gelling agents, novel injectable chitosan-based biocompatible thermogels (CTGels) having excellent mechanical properties and cytocompatibility have been developed. Three thermogel formulations with acceptable physicochemical properties, such as physiological pH and osmolality, macroporosity, and gelation rates were compared.The CTGel2 formulation outperformed the others by providing an environment suitable for the encapsulation of viable CD8 + T lymphocytes, supporting their proliferation and gradual release. In addition, the encapsulated T cell phenotypes were influenced by surrounding conditions and by tumor cells, while maintaining their capacity to kill tumor cells. This strongly suggests that cells encapsulated in this formulation retain their anti-cancer functions, and that this locally injectable hydrogel may be further developed to complement a wide variety of existing immunotherapies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chitosan thermogels for local expansion and delivery of tumor-specific T lymphocytes towards enhanced cancer immunotherapies

et al. 2016

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Chitosan‐Based Drug Delivery Systems

Peptu

Humelnicu

Rotaru

et al. 2019

Chitin and Chitosan

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Biodegradable Polymers for Biomedical and Tissue Engineering

Pushpamalar

Paramasivam

Meganathan

et al. 2020

Materials Science and Technology

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Currently, scientists are on the cutting edge of inventing many novel biomaterials from various sources for selection in tissue engineering and controlled drug delivery systems to improvise the patient surgical and treatment processes. This article focuses on the significant improvements made in the design of natural, biodegradable polymer‐based biomaterials and their applications in biomedical and tissue engineering areas. Polysaccharide and protein‐based hydrogels are suitable for soft and hard biomaterials. The soft biomaterial is made of biodegradable natural or synthetic or in a combination of both polymers that can be utilized for a stipulated time and cleared partly or whole of the system that it treats, enhance the rate of healing or replace tissues or organs. Soft biomaterials fabricated for muscle, ligament, tendon, articular cartilage tissue engineering exhibit valuable properties, such as biocompatibility, biodegradability, flexible, renewability, and cheaper. The combination of the polysaccharides and proteins with synthetic biodegradable polymers improved the mechanical strength and when added with inorganic calcium compounds could be exploited in hard tissue engineering, such as bone tissue engineering. The development of biomaterial for scaffolding in tissue engineering using polysaccharides and proteins could lead to the emergence of a new era of safer material to replace the damaged tissues in the body without suffering inflammation. Polysaccharides and proteins are consumed as food, and treating damaged tissue with food‐based biomaterial would not harm the humankind. In the near future, the development of natural polymer‐based biodegradable biomaterials by interdisciplinary and multidisciplinary research scientists is highly important.

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Injectable thermosensitive chitosan hydrogels with controlled gelation kinetics and enhanced mechanical resistance

Cited by 126 publications

References 22 publications

Chitosan thermogels for local expansion and delivery of tumor-specific T lymphocytes towards enhanced cancer immunotherapies

Chitosan thermogels for local expansion and delivery of tumor-specific T lymphocytes towards enhanced cancer immunotherapies

Chitosan‐Based Drug Delivery Systems

Biodegradable Polymers for Biomedical and Tissue Engineering

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