Injectable Thermosensitive Gel CH‐BPNs‐NBP for Effective Periodontitis Treatment through ROS‐Scavenging and Jaw Vascular Unit Protection

Abstract

Periodontitis, a prevalent inflammatory condition in the oral cavity, is closely associated with oxidative stress‐induced tissue damage mediated by excessive reactive oxygen species (ROS) production. The jaw vascular unit (JVU), encompassing both vascular and lymphatic vessels, plays a crucial role in maintaining tissue fluid homeostasis and contributes to the pathological process in inflammatory diseases of the jaw. In this study, we present a novel approach for treating periodontitis through the development of an injectable thermosensitive gel (CH‐BPNs‐NBP). The gel formulation incorporated black phosphorus nanosheets (BPNs), which are notable for their ROS‐scavenging properties, and dl‐3‐n‐butylphthalide (NBP), a vasodilator that promotes lymphatic vessel function within the JVU. Our results demonstrated that the designed thermosensitive gel served as a controlled release system, delivering BPNs and NBP to the site of inflammation. CH‐BPNs‐NBP not only protected macrophages and human lymphatic endothelial cells from ROS attack but also promoted M2 polarization and lymphatic function. In in vivo studies, we observed a significant reduction in inflammation and tissue damage, accompanied by a notable promotion of alveolar bone regeneration. This research introduces a promising therapeutic strategy for periodontitis, leveraging the unique properties of BPNs and NBP within an injectable thermosensitive gel.This article is protected by copyright. All rights reserved