Injection Matching and Dilution Studies

Lemaire, J.; Raka, E.

doi:10.2172/1129727

Cited by 13 publications

(21 citation statements)

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“…The double-stranded RNA immunostimulant polyI:C can signal through TLR3 on endosomes (12), RNA-activated protein kinase R (13,14) and MDA-5 ⁄ RIG-I-like receptors (15,16). We have demonstrated that polyI:C mediates both proinflammatory and cytotoxic effects in keratinocytes through TLR3-dependent mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Oligodeoxynucleotides inhibit Toll‐like receptor 3 mediated cytotoxicity and CXCL8 release in keratinocytes

Grimstad

Pukstad

Stenvik

et al. 2011

Experimental Dermatology

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Toll‐like receptor 3 (TLR3) is an important sensor of viral infections and injury of self in keratinocytes. In this study, we stimulated primary keratinocytes with the TLR3‐ligand polyI:C. This induced a toxic effect shown by up‐regulation of the alarmin high‐mobility group protein B1 and reduced responses in a MTT‐assay. PolyI:C was a potent inducer of proinflammatory cytokines, and both these responses and the cytotoxic effects were found to be TLR3 dependent, as demonstrated by the use of siRNA for TLR3. Interestingly, co‐stimulation with oligodeoxynucleotides (ODNs) inhibited all polyI:C induced effects. This inhibition was found to be mediated by the competition of endocytic uptake of polyI:C and ODNs. We have found polyI:C induced cytotoxicity and proinflammatory responses to be dependent of TLR3 and that this may be inhibited by ODNs. With these findings, we see a promising potential for ODNs in inhibiting TLR3‐induced responses in inflammatory skin disorders.

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Section: Discussionmentioning

confidence: 99%

Oligodeoxynucleotides inhibit Toll‐like receptor 3 mediated cytotoxicity and CXCL8 release in keratinocytes

Grimstad

Pukstad

Stenvik

et al. 2011

Experimental Dermatology

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“…The intensity of these peaks is directly related to the degree of photooxidation of the PA11 matrix. Further, a small absorption band located at λ max = 1,713 cm −1 is also noticed, especially at longer exposure time, corresponding to carboxylic acid groups [27,28]. Accordingly, the photooxidation mechanism of PA11 leads to the formation of imide products resulting from the photochemical decomposition of hydroperoxides through the homolysis of the peroxide bonds [26].…”

Section: Nanocomposite Morphologymentioning

confidence: 94%

“…The latter can be observed at λ max = 1,713 cm −1 in the FTIR spectra of both irradiated PA11 and PA11/UVMC nanocomposites. Although the nature of the oxidized groups responsible for the discoloration of PA 11 is still in debate [31], the UV absorbing products are likely attributed to the formation of conjugated carbonyls resulting from the crotonization of aldehydes [27,28]. Figure 7 shows the kinetic curves of the YI for the neat PA11 and PA11/UVMC nanocomposite samples up to 600 h. A fast growth of the YI at the beginning of exposure up to almost 200 h, before slowing slightly, is observed.…”

Section: Evaluation Of Yellowingmentioning

confidence: 99%

A degradation study of polyamide 11/vermiculite nanocomposites under accelerated UV test

Kaci

Dehouche

Merwe

2019

Polymer Engineering & Sci

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Accelerated photooxidation under ultraviolet (UV) test of polyamide 11 (PA11) films filled with unmodified vermiculite clay at 5 wt% was investigated up to 600 h. Film samples of ~60‐μm thick were prepared by melt compounding using a cast extruder and exposed to UV light irradiation at λ > 295 nm. Fourier transform infrared (FTIR) spectra indicated similar structural changes occurring in both PA11 and PA11/unmodified vermiculite nanoclay (UVMC) nanocomposite along the photooxidation process, resulting in imides and carboxylic acids as the main carbonyl products. It was however observed that the formation rate of carbonyls in the PA11/UVMC nanocomposite was slower than neat PA11. This behavior is consistent with the yellowing index evolution determined by ultraviolet–visible (UV–vis) spectroscopy. Further, the photooxidation stability of the samples was also evaluated by the onset oxidation temperature determined by differential scanning calorimetry. The results indicated a better stability of the nanocomposite film than neat PA11, corroborating well the data obtained by FTIR and UV–vis techniques. POLYM. ENG. SCI., 59:2449–2457, 2019. © 2019 Society of Plastics Engineers

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“…The production of almost all of the studied cytokines appears to be dependent on the concentration of Larifan used, with the efficacy of induction or suppression decreasing when higher concentrations are used. This "bell-shaped" response type is well known in the context of dsRNA-induced IFN production [21] and can be explained by the activation mechanism that involves dimerization and multimerization with dsRNA molecules for most of the dsRNA detecting receptors [22][23][24][25]. It is of interest that, among molecules suppressed by Larifan, there clearly appeared different concentration dependencies, with 200 μg/mL of Larifan being the most effective concentration for suppressing IL-16 and 400 μg/mL for suppressing IP-10 and SDF1-a+b.…”

Section: Figmentioning

confidence: 99%

Ex vivo cytokine production in peripheral blood mononuclear cells after their stimulation with dsRNA of natural origin

Veinalde

Petrovska

Brüvere

et al. 2014

Biotech and App Biochem

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Double-stranded RNA (dsRNA) is a pathogen-associated molecular pattern, known for its ability to induce antiviral response and enhance communication between cells mediating innate and adaptive immune responses. The aim of this study was to characterize the effect of the dsRNA-containing product Larifan on the production of a wide spectrum of cytokines and chemokines in ex vivo cultivated peripheral blood mononuclear cells. Concentrations of 29 different cytokines were detected by a Luminex® 200™ System using three Milliplex MAP Multiplex Assay Kits. Larifan caused strong induction of chemokine macrophage inflammatory protein 1β, I-309, and TARC, proinflammatory cytokines IL-6, tumor necrosis factor -α, granulocyte macrophage colony-stimulating factor, anti-inflammatory IL-10, and cellular immunity mediating factors IL-23 and interferon-γ. Considerable suppression of IL-16 and chemokine stromal cell-derived factor 1 a+b and interferon gamma-induced protein 10 was also observed. The network of molecules responding to the presence of Larifan revealed the pleiotropic effect this product exerts on immune response.

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Injection Matching and Dilution Studies

Cited by 13 publications

References 0 publications

Oligodeoxynucleotides inhibit Toll‐like receptor 3 mediated cytotoxicity and CXCL8 release in keratinocytes

Oligodeoxynucleotides inhibit Toll‐like receptor 3 mediated cytotoxicity and CXCL8 release in keratinocytes

A degradation study of polyamide 11/vermiculite nanocomposites under accelerated UV test

Ex vivo cytokine production in peripheral blood mononuclear cells after their stimulation with dsRNA of natural origin

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