Injection of Antibodies into Grasshopper Eggs as a Method for Studying Embryonic Development

Petrey, Dwayne; Buster, Dan; Donato, Katja K.; Anderson, Hilary

doi:10.1111/j.1440-169x.1989.00299.x

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…The time lag of about 1 day between controls and antiserum-treated larvae agrees well with the findings that antibody complexes remain stable for at least 24 h after injection into developing insect eggs [9]. Increasing degradation of the antibodies after this time would allow normal development and pupation with the delay of 1 day, as shown.…”

Section: Discussionsupporting

confidence: 87%

Temporal molt inhibition by anti‐ecdysone serum injected into lepidopteran larvae

Birkenbeil

Eckert

1991

Arch Insect Biochem Physiol

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Injections of an anti-ecdysone serum into Galleria mellonella L. after the beginning of the wandering stage of the last instar resulted in delayed pupation.Injections before this time did not produce this effect and simultaneous injection of antiserum together with 20-hydroxyecdysone leads to immediate initiation of molt. lmmunocytochemical studies demonstrated the anti-ecdysone serum specifically bound to the prothoracic glands labeling the peripheral extracellular lacunar system and sites of ecdysteroid release.

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Section: Discussionsupporting

confidence: 87%

Temporal molt inhibition by anti‐ecdysone serum injected into lepidopteran larvae

Birkenbeil

Eckert

1991

Arch Insect Biochem Physiol

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“…The method for in vivo injections was based on fly embryo injection protocols and was similar to that described by Petrey et al (1989). Needles were filled with a solution consisting of 12.5 mM HA and food dye in 50% DMSO/40% ethanol/10% aqueous solution.…”

Section: In Vivo Egg Injectionsmentioning

confidence: 99%

Tyrosine kinase inhibition produces specific alterations in axon guidance in the grasshopper embryo

Menon

Zinn

1998

Development

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Tyrosine kinase signaling pathways are essential for process outgrowth and guidance during nervous system development. We have examined the roles of tyrosine kinase activity in programming growth cone guidance decisions in an intact nervous system in which neurons can be individually identified. We applied the tyrosine kinase inhibitors herbimycin A and genistein to whole 40% grasshopper embryos placed in medium, or injected the inhibitors into intact grasshopper eggs. Both inhibitors caused interneuronal axons that normally would grow along the longitudinal connectives to instead leave the central nervous system (CNS) within the segmental nerve root and grow out toward the body wall muscles. In addition, herbimycin A produced pathfinding errors in which many longitudinal axons crossed the CNS midline. To study how this drug affected guidance decisions made by individual growth cones, we dye-filled the pCC interneuron, which normally extends an axon anteriorly along the ipsilateral longitudinal connective. In the presence of herbimycin A, the pCC growth cone was redirected across the anterior commissure. These phenotypes suggest that tyrosine kinase inhibition blocks a signaling mechanism that repels the growth cones of longitudinal connective neurons and prevents them from crossing the midline.

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Laser inactivation of fasciclin I disrupts axon adhesion of grasshopper pioneer neurons

Jay

Keshishian

1990

Nature

107

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A molecular mechanism for selective axonal adhesion is a central question of neural development. Cell adhesion molecules have been identified, but it has been difficult to ascribe functions for these proteins in vivo. Here we show that the neuronal membrane glycoprotein fasciclin I has a role in the adhesion of sister axons during the development of the grasshopper limb bud. To do this we used a new technique, chromophore-assisted laser inactivation (CALI), which causes the precisely timed thermal denaturation of specific proteins by laser light targeted through a dye-labelled antibody, without any other observable damage to living cells. This can be achieved by relaxation of the laser-excited dye which releases heat to denature the bound protein; the rapid dissipation of heat with distance insulates unbound proteins from damage. CALI is a molecular analogue of cellular laser ablation and provides an unprecedented level of spatial and temporal resolution. Using dye-labelled antibodies that recognize fasciclin I, CALI disrupts fasciculation of the pioneer neurons without affecting their growth or guidance.

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Injection of Antibodies into Grasshopper Eggs as a Method for Studying Embryonic Development

Cited by 3 publications

References 15 publications

Temporal molt inhibition by anti‐ecdysone serum injected into lepidopteran larvae

Temporal molt inhibition by anti‐ecdysone serum injected into lepidopteran larvae

Tyrosine kinase inhibition produces specific alterations in axon guidance in the grasshopper embryo

Laser inactivation of fasciclin I disrupts axon adhesion of grasshopper pioneer neurons

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