Injection of lipopolysaccharide induces the migration of splenic neutrophils to the T cell area of the white pulp: role of CD14 and CXC chemokines

Abstract

There is increasing evidence that neutrophils are involved in the regulation of adaptive immunity. We therefore tested whether these cells may colocalize with T lymphocytes in lymphoid organs. Our results demonstrate that administration of the microbial product LPS induces the migration of neutrophils in the spleen from the red pulp and the marginal zone to the area of the white pulp where T cells reside. This movement is CD14-dependent, whereas the recruitment of neutrophils in the peritoneal cavity is increased in the absence of CD14. Our data further suggest the involvement of the chemokine MIP-2 and keratinocyte-derived chemokine and their receptor CXCR2. We conclude that neutrophils may interact with naïve T cells upon infection/inflammation and that the migration of neutrophils in the lymphoid organs and in the periphery is regulated differently by a signal transduced by CD14 J. Leukoc. Biol. 83: 640 -647; 2008.

Microbe-Specific Unconventional T Cells Induce Human Neutrophil Differentiation into Antigen Cross-Presenting Cells

“…Expression of the lymph node homing receptor CCR7 by activated neutrophils was reported before (30) but could not be confirmed in the current study (data not shown). Still, APClike neutrophils may also gain access to inflamed draining lymph nodes through the action of inflammatory chemokines (7)(8)(9)(10). Irrespective of the anatomical context, APC-like neutrophils may contribute to protective immune responses, by fighting the "first hit" infection as a result of inducing Ag-specific CD4 + and CD8 + T cells and by harnessing the T cell compartment against potential "second hit" infections.…”

“…As such, neutrophils regulate the recruitment and function of various cell types and interact with immune and nonimmune cells. Intriguingly, neutrophils directly affect Ag-specific responses by facilitating monocyte differentiation and dendritic cell maturation, and by interacting with T cells and B cells (4)(5)(6)(7)(8)(9)(10). Murine neutrophils have been shown to present Ags to both CD4 + and CD8 + T cells (11)(12)(13), and to differentiate into neutrophil-dendritic cell hybrids in vitro and in vivo (14,15).…”

Metabolic Stress Boosts Humoral Responses In Vivo Independently of Inflammasome and Inflammatory Reaction

“…Immunohistochemistry was performed as described previously (24). Briefly, spleens were fixed in ImmunoHistoFix (Aphase, Nassogne, Belgium), embedded in ImmunoHistoWax (Aphase), and sectioned at 3-6 mm.…”

Changes in count and function of splenic lymphocytes from patients with portal hypertension

“…A possible reason is that long-term contact between noxious substances, such as endotoxin and hepatitis virus, and spleen tissue has promoted activation and hyperplasia of lymphocytes in the spleen [16,25] . Also, this contact has enhanced its function to maximize the elimination of toxins in the body and maintain body balance [26][27][28][29] .…”