Injection of Platelet Rich Plasma Intra-Ovarian to Treat Inactive Ovaries in Rabbits

Abstract

In this investigation, we examined the efficiency of PRP as a therapy for inactive ovary (IO) in rabbits. IO in rabbits was induced by administration of D-galactose (200mg/kg) (dissolved in saline, 0.2mL/rabbits/day) for ten consecutive days. Rabbits divide into three groups. After preparing two concentrations of PRP solution, the PRP was injected intraovarian through surgical intervention. Then two weeks later, FSH and estrogen were measured, histopathological examination for ovaries were made according to the routine procedure, and finally, the mating trial was made to evaluate the fertility status. Ten days after D-galactose administration IO had significantly higher levels of FSH (2.30±1.32) and (2.80±0.80) and lower levels of E2 (5.55±0.78) and (5.36±0.91), in comparison to the control group (P<0.05) (1.99±0.80 and 7.16±1.195) respectively. The results after injection of PRP in two different concentrations indicated that the highest value significant (P<0.05) was in E2 levels (7.87±0.9) and (8.08±2.21), and the lowest significant (P<0.05) was in FSH levels (1.60±0.57) and (1.49±0.38) comparison with others induction D-galactose. Histopathological examination showed an increase in follicles in PRP groups compared to galactose group. It was concluded that PRP had a protective effect against galactose toxicity and improved follicular growth.