Injection technique and scanning electron microscopic study of the arterial pattern of the 20 gestation days (G20) rat fetus

Navarro, Marc; Carretero, Ana; Canut, Lourdes; Pérez‐Aparicio, Francisco Javier; Cristòfol, Carles; Manesse, Maël; Sautet, J.; Arboix, M.; Ruberte, Jesús

doi:10.1258/002367798780559347

Cited by 4 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 Aortic arch; 2 brachiocephalic trunk; 2¢ right subclavian artery; 2¢¢ right common carotid artery; 3 left common carotid artery; 4 left subclavian artery last criterion to be a good marker of the complete filling of the vessels (Christofferson and Nilsson 1988;Castelholz 1989;Hodde and Nowell 1980). In our study, the success rate of 46.5% of microvascular cast injections was less important than expected since previous studies had provided a success rate of 65% when rat fetuses at the 20th gestation day had been injected (Navarro et al 1997). Before being anesthetized, some of the rat fetuses at the 21st gestation day breathed when they were pulled out from the uterus.…”

Section: Technical Aspectmentioning

confidence: 53%

“…The other end of the pipette was attached to a 50-ml syringe, which in turn was connected to a mechanical pump (Harvard Apparatus, Mass., USA). As previously described (Navarro et al 1997), the fetus was then injected under binocular control with mercox resin (Mercox-Japan Vilene, Tokyo, Japan) until its vascular system was filled.…”

Section: Methodsmentioning

confidence: 99%

“…From the simple morphological examination of our casts, it was observed that the most frequent (98.1%) arterial branching pattern consisted of three vessels arising from the aortic arch: the brachiocephalic trunk, the left common carotid artery, and the left subclavian artery; it corresponds to the classic treatises of the anatomy of the rat (Smith and Calhoun 1968;Rowet 1974;Hebel and Stromberg 1976;Wingerd 1988;Popesko et al 1992) and specific studies dealing with the vascular anatomy of the rat (Navarro et al 1997). In one case, only (1.9%), the brachiocephalic trunk was absent (Fig.…”

Section: Variation or Anomalymentioning

confidence: 95%

“…The normal anatomy of the rat's aortic arch is only briefly described in some classical anatomy books (Smith and Calhoun 1968;Rowett 1974;Popesko et al 1992) but more specific articles report some precisions about the vascular anatomy of the rat (Navarro et al 1997) and other laboratory species such as the rabbit (Angell-James 1974; Noden and De Lahunta 1985).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mophometric study of the aortic arch and its major branches in rat fetuses on the 21st day of gestation

et al. 2005

Self Cite

View full text Add to dashboard Cite

The anatomy and embryology of the aortic arch and its branching tributaries (brachiocephalic trunk, left common carotid artery and left subclavian artery) in man and animals are well substantiated. However, the anatomical variations and morphometry of the aortic arch and its branching tributaries in rat fetus at the 21st gestation day have not been studied. Pregnant rats were hysterectomized and the arterial systems of 114 fetuses were injected with a polymerisable resin through the umbilical artery. After maceration, the vascular casts were dissected out and prepared for observations under a scanning electron microscope (SEM). The resulting SEM pictures were studied with a picture analyser and different vessel parameters (diameters, lengths and angles) were measured. The success rate of the microvascular cast injection was 46.5%. Out of the 53 observed aortic arch casts, 98.1% showed the classical branching pattern and one (1.9%) had no brachiocephalic trunk. Morphological analysis showed many differences, which were not linked to the litter. The statistical processing of the measurements enabled us to determine that the aorta diameter after the branching of the left subclavian artery was the most replicable parameter. Moreover, the results revealed some strong correlations between different parameters. There are probably no discrete categories among the various observed parameters as diameters and angles. Some parameters show very little variability and can thus be used as reference points for further studies such as the comparison of a control population with a population treated with a relevant xenobiotic.

show abstract

Section: Technical Aspectmentioning

confidence: 53%

Section: Methodsmentioning

confidence: 99%

Section: Variation or Anomalymentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mophometric study of the aortic arch and its major branches in rat fetuses on the 21st day of gestation

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…Additionally, SEM of casts of embryonic vascular systems has become a standard method for studying the fine details of blood vessels (Navarro et al, 1998). All of the specimens in this study were examined at an accelerating voltage of 10 kV with an Amray (Bedford, MA) 1000-B scanning electron microscope.…”

Section: Sem Of the Vascular Castsmentioning

confidence: 99%

Embryonic origin of the caudal mesenteric artery in the mouse

Gest

Carron

2003

Anat. Rec.

View full text Add to dashboard Cite

It is commonly held that the caudal mesenteric artery (CaMA, or inferior mesenteric artery in humans) arises in the same manner as the celiac and cranial mesenteric artery (CrMA, or superior mesenteric artery in humans), i.e., from the remodeling of the vitelline system of arteries that surrounds and supports the yolk sac. Conflicting evidence about the precise manner in which the CaMA arises was presented in studies of the luxate syndrome (Carter: J. Genet. 1954;52:1-35) and sirenomelia (Schreiner and Hoornbeek: J. Morphol. 1973;141:345-358) in the mouse. These studies suggested that the CaMA arises from the remodeling of the medial umbilical arterial roots. Later studies of blood vessel development in the hindlimb of the Dominant hemimelic mouse (Gest: Anat. Rec. 1984;208:296; Anat. Rec. 1987;218:49A; Gest and Roden: Anat. Rec. 1988;220:37-38A) also supported the results of the previous studies. The present investigation tests the hypothesis that the CaMA arises as a result of the regression and remodeling of the medial umbilical arterial roots. Vascular corrosion casts of 9.5-13.5-day-old mouse embryos were observed by scanning electron microscopy (SEM). The results of the present investigation agree with the aforementioned studies. The medial umbilical roots initially conduct the blood to the placenta. On days 10-12 the medial umbilical roots regress and remodel into the CaMA, while the lateral umbilical roots take over the blood supply to the placenta. On the basis of our results, we conclude that the CaMA arises from the medial umbilical roots and not from the remodeling of the vitelline system of arteries, as previously assumed. Anat Rec Part A 271A:192-201, 2003.

show abstract

Melanoma xenotransplant on the chicken chorioallantoic membrane: a complex biological model for the study of cancer cell behaviour

Strnadová

Španko

Dvořánková

et al. 2020

Histochem Cell Biol

View full text Add to dashboard Cite

Injection technique and scanning electron microscopic study of the arterial pattern of the 20 gestation days (G20) rat fetus

Cited by 4 publications

References 20 publications

Mophometric study of the aortic arch and its major branches in rat fetuses on the 21st day of gestation

Mophometric study of the aortic arch and its major branches in rat fetuses on the 21st day of gestation

Embryonic origin of the caudal mesenteric artery in the mouse

Melanoma xenotransplant on the chicken chorioallantoic membrane: a complex biological model for the study of cancer cell behaviour

Contact Info

Product

Resources

About