Injections of recombinant human erythropoietin increases lactate influx into erythrocytes

Connes, Philippe; Caillaud, Corinne; Mercier, Jacques; Bouix, D; Casties, Jean-François

doi:10.1152/japplphysiol.00715.2003

Cited by 19 publications

(25 citation statements)

References 42 publications

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“…The values found for RBC lactate transport activity at high external [La] and for V max were lower than the values reported by previous studies in humans (6,7,21,29). In horses, some studies reported that RBC lactate transport activity might be dependent on the breed (32,33).…”

Section: Discussioncontrasting

confidence: 67%

“…Ethnic differences rather than the accuracy of the measures may thus be at the origin of the lower values found in the present study for V max and RBC lactate transport activity at high [La]. We used exactly the same methodology as Skelton et al (28,29) and Connes et al (6,7). Moreover, the fractional contribution of MCT-1 to total lactate transport activity decreased with the rise in external [La], reflecting the saturation properties of the MCT-1 pathway, as already reported by previous studies (6,7,9,21,28,29).…”

Section: Discussionmentioning

confidence: 99%

“…However, it is notable that the main difference between the two studies is the level of physical fitness in the groups. If training status and aerobic physical fitness influence the activity of RBC lactate transport (7,28,29), one assumes that final conclusions could not be drawn from Pattillo and Gladden's study (21) regarding the role of exercise on RBC lactate transport activity in SCT carriers.…”

Section: Discussionmentioning

confidence: 99%

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Faster lactate transport across red blood cell membrane in sickle cell trait carriers

Farhang¹,

Connes²,

Hue³

et al. 2006

Journal of Applied Physiology

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Section: Discussioncontrasting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Faster lactate transport across red blood cell membrane in sickle cell trait carriers

Farhang¹,

Connes²,

Hue³

et al. 2006

Journal of Applied Physiology

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“…The underlying physiological mechanism is still unknown but may be related to erythropoietin (Epo) secretion. In a recent study (7), our laboratory showed that 4 wk of treatment with recombinant human Epo led to a significant increase in RBC lactate influx in human subjects. A potential role for Epo in MCT-1 expression might also explain why Juel et al (18) found an increase in MCT-1 expression on RBC membrane but did not find such a change in muscle samples.…”

Section: Lactate Influxmentioning

confidence: 98%

Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes?

Connes¹,

Bouix²,

Py³

et al. 2004

Journal of Applied Physiology

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. Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes? J Appl Physiol 97: 1053-1058. First published April 30, 2004 10.1152/japplphysiol.00993.2003.-This study investigated 1) red blood cells (RBC) rigidity and 2) lactate influxes into RBCs in endurance-trained athletes with and without exercise-induced hypoxemia (EIH). Nine EIH and six non-EIH subjects performed a submaximal steady-state exercise on a cycloergometer at 60% of maximal aerobic power for 10 min, followed by 15 min at 85% of maximal aerobic power. At rest and at the end of exercise, arterialized blood was sampled for analysis of arterialized pressure in oxygen, and venous blood was drawn for analysis of plasma lactate concentrations and hemorheological parameters. Lactate influxes into RBCs were measured at three labeled [U-14 C]lactate concentrations (1.6, 8.1, and 41 mM) on venous blood sampled at rest. The EIH subjects had higher maximal oxygen uptake than non-EIH (P Ͻ 0.05). Total lactate influx was significantly higher in RBCs from EIH compared with non-EIH subjects at 8.1 mM (1,498.1 Ϯ 87.8 vs. 1,035.9 Ϯ 114.8 nmol ⅐ ml Ϫ1 ⅐ min Ϫ1 ; P Ͻ 0.05) and 41 mM (2,562.0 Ϯ 145.0 vs. 1,618.1 Ϯ 149.4 nmol ⅐ ml Ϫ1 ⅐ min Ϫ1 ; P Ͻ 0.01). Monocarboxylate transporter-1-mediated lactate influx was also higher in EIH at 8.1 mM (P Ͻ 0.05) and 41 mM (P Ͻ 0.01). The drop in arterial oxygen partial pressure was negatively correlated with total lactate influx measured at 8.1 mM (r ϭ Ϫ0.82, P Ͻ 0.05) and 41 mM (r ϭ Ϫ0.84, P Ͻ 0.05) in the two groups together. Plasma lactate concentrations and hemorheological data were similar in the two groups at rest and at the end of exercise. The results showed higher monocarboxylate transporter-1-mediated lactate influx in the EIH subjects and suggested that EIH could modify lactate influx into erythrocyte. However, higher lactate influx in EIH subjects was not accompanied by an increase in RBC rigidity. monocarboxylate transporter; endurance; lactate metabolism; hypoxemia; hemorheology TRANSPORT OF LACTATE ACROSS the erythrocyte membrane proceeds by three distinct pathways (9): 1) nonionic diffusion of the undissociated acid; 2) an inorganic anion-exchange system, often referred to as the band 3 system; and 3) a monocarboxylate-specific carrier mechanism (23). Juel et al. (18) recently showed that monocarboxylate transporter-1 (MCT-1) and band 3 expressions were increased with chronic hypoxia exposure, suggesting that these proteins may be upregulated by hypoxia.The functional significance of the hypoxia-induced changes is likely an increase of lactate and H ϩ fluxes from plasma to erythrocyte. During sea-level exercise, some endurance-trained athletes experience arterial hypoxemia [exercise-induced hypoxemia (EIH)] that can be defined as a decrease in both oxygen arterial partial pressure (Pa O 2 ) and arterial hemoglobin saturation during exercise (8). Miyachi and Katayama (21) have reported repeated episodes of EIH during training sessions when endurance athlet...

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“…Very little is also known about the regulation of MCT genes. Leptin has been shown to upregulate MCT1 mediated butyrate uptake in colonocytes (Buyse, Sitaraman, Liu, Bado, & Merlin, 2002) and erythropoietin increases the amount of MCT1 protein in erythrocyte membranes (Connes, Caillaud, Mercier, Bouix, & Casties, 2004).…”

Section: Monocarboxylate Transportersmentioning

confidence: 99%

Carbohydrate metabolism in meat animals

2005

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Oxidative energy production is by far dominant in living animal muscles, with the exception the short periods of severe stress, where the aerobic capacity is exceeded, and formation of large amounts of lactic acid will take place. Energy consumption in muscle cells continues post mortem with formation of large amounts of lactate and formation of protons, because the aerobic processes for energy production are not available. Post mortem, the fall in pH is delayed only by buffering capacity of the muscle fibres. In living animals, in addition to buffering capacity, both respiration and transport of lactate and protons out of the muscle fibres by monocarboxylate transporters participate in the regulation of muscle fibre pH which never falls as low as the ultimate pH of the meat. Understanding the regulation of pH in muscle is important both for the welfare of living animals and from the technological point of view as a factor influencing meat quality.

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Injections of recombinant human erythropoietin increases lactate influx into erythrocytes

Cited by 19 publications

References 42 publications

Faster lactate transport across red blood cell membrane in sickle cell trait carriers

Faster lactate transport across red blood cell membrane in sickle cell trait carriers

Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes?

Carbohydrate metabolism in meat animals

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