Introduction: Dental Health Care Providers (DCPs) traditionally use finger retraction or mirror retraction when delivering local anesthesia. Needlestick injuries (NSIs) occur as hand retracting mucosa is likely to experience injury during dental anesthesia delivery. The aim of this paper is to examine a clinical split mouth study comparing two retraction methods and DCP's retraction preference during delivery of dental anesthesia. The clinical implications from this study impact the practitioner's risk of experiencing an NSI. Methodology: The IRB approved study (clinical trials ID: NCT02414620) compared the comfort and ease of the retraction method used while delivering an anterior superior alveolar (ASA) injection bilaterally. Surveys given to participants asked about comfort and ease of use of retraction methods, as well as preferred method of retraction during anesthesia delivery. Chisquare tests of goodness-of-fit were conducted to investigate whether there was a significant difference in the proportion of respondents that chose the various categories within one criterion. Results: 62 DCPs participated in the study and no reported NSIs. Data from the DCPs report significance (p value < 0.001) in comfort comparing retraction methods when providing anesthesia. Regarding preference of retraction, 22 prefer mirror, 29 preferred device, and 3 preferred their finger. Regarding ease of retraction used, 30 chose mirror, 18 device, and 10 chose finger. Conclusion: Our hypothesis and clinical implication were confirmed. More studies need to be conducted regarding the benefits of using a fingerless retraction method and its effectiveness in dental anesthesia.