Inkjet 3D printed chip for capillary gel electrophoresis

Walczak, Rafał; Adamski, Krzysztof; Kubicki, Wojciech

doi:10.1016/j.snb.2018.01.174

Cited by 30 publications

(17 citation statements)

References 33 publications

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“…Although these devices show promise in working with multiple materials and integrating steps in one chip, the detection limit (2 ppm) is too high for many bioanalyses, and the ability to multiplex medically relevant analytes has yet to be demonstrated. Walczak et al 31 3D printed ∼400 μm diameter microchannels, which they filled with gel to electrophoretically analyze a fluorescently labeled DNA ladder. However, the channel fabrication suffered from multihour clearing of support materials.…”

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confidence: 99%

3D Printed Microfluidic Devices for Microchip Electrophoresis of Preterm Birth Biomarkers

et al. 2019

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This work demonstrates for the first time the creation of microchip electrophoresis devices with ~50 µm cross sectional dimensions by stereolithographic 3D printing and their application in the analysis of medically significant biomarkers related to risk for preterm birth (PTB). We determined that device current was linear with applied potential up to 800 V (620 V/cm). We optimized device and separation conditions using fluorescently labeled amino acids as a model system and compared the performance in our 3D printed microfluidic devices to that in other device materials commonly used for microchip electrophoresis analysis. We demonstrated for the first time microchip electrophoresis in a 3D printed device of three PTB biomarkers, including peptides and a protein, with suitable separation characteristics. Limits of detection for microchip electrophoresis in 3D printed microfluidic devices were also determined for PTB biomarkers to be in the high picomolar to low nanomolar range.

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confidence: 99%

3D Printed Microfluidic Devices for Microchip Electrophoresis of Preterm Birth Biomarkers

et al. 2019

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“…Walczak et al introduced 3D inkjet printing to integrate capillary gel electrophoresis into their sensor systems. [93] In these studies, T-shaped gel electrophoresis assays fabricated from VisiJet resin and 3D inkjet printing were employed (Figure 7c). This resin is a frequently used material for the fabrication of microfluidic devices because of its transparent properties.…”

Section: Printed Microfluidicsmentioning

confidence: 99%

Inkjet Printing and 3D Printing Strategies for Biosensing, Analytical, and Diagnostic Applications

2022

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Inkjet printing and 3D inkjet printing have found many applications in the fabrication of a great variety of devices, which have been developed with the aim to improve and simplify the design, fabrication, and performance of sensors and analytical platforms. Here, developments of these printing technologies reported during the last 10 years are reviewed and their versatile applicability for the fabrication of improved sensing platforms and analytical and diagnostic sensor systems is demonstrated. Illustrative examples are reviewed in the context of particular advantages provided by inkjet printing technologies. Next to aspects of device printing and fabrication strategies, the utilization of inkjet dispensing, which can be implemented into common analytical tools utilizing customized inkjet printing equipment as well as state‐of‐the‐art consumer inkjet printing devices, is highlighted. This review aims to providing a comprehensive overview of examples integrating inkjet and 3D inkjet printing technologies into device layout fabrication, dosing, and analytical applications to demonstrate the versatile applicability of these technologies, and furthermore, to inspire the utilization of inkjet printing for future developments.

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“…PJ has recently been utilized to 3D print devices for biochemical applications, including gel electrophoresis. Walczak et al 168 used a VisiJet resin to make T-shaped devices for gel electrophoresis assays. Channels had better resolution when printed in high-compared to standard-definition mode, but the smallest channels still had several hundred micrometer widths.…”

Section: ■ Three-dimensional Printingmentioning

confidence: 99%

Microfluidics: Innovations in Materials and Their Fabrication and Functionalization

et al. 2019

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Inkjet 3D printed chip for capillary gel electrophoresis

Cited by 30 publications

References 33 publications

3D Printed Microfluidic Devices for Microchip Electrophoresis of Preterm Birth Biomarkers

3D Printed Microfluidic Devices for Microchip Electrophoresis of Preterm Birth Biomarkers

Inkjet Printing and 3D Printing Strategies for Biosensing, Analytical, and Diagnostic Applications

Microfluidics: Innovations in Materials and Their Fabrication and Functionalization

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