Inkjet fabrication of hydrogel microarrays using in situ nanolitre-scale polymerisation

Zhang, Rong; Liberski, Albert Ryszard; Khan, Ferdous; Díaz‐Mochón, Juan José; Bradley, Mark

doi:10.1039/b717932d

Cited by 63 publications

(56 citation statements)

References 29 publications

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“…The concept of inkjet fabrication of hydrogel microarrays has been described previously [26], but to fulfil the current purpose, was extensively modified. Microscope glass slides (76 Â 26 mm) (Menzel GmbH Co. KG) were cleaned with an O 2 plasma (Europlasma) for 10 min with an oxygen pressure of 1.5 bar.…”

Section: Preparation Of Polymer Hydrogel Microarraysmentioning

confidence: 99%

Microarrays of over 2000 hydrogels – Identification of substrates for cellular trapping and thermally triggered release

et al. 2009

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Section: Preparation Of Polymer Hydrogel Microarraysmentioning

confidence: 99%

Microarrays of over 2000 hydrogels – Identification of substrates for cellular trapping and thermally triggered release

et al. 2009

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“…Ink-jet printing was first used to prepare a polymer array from individually deposited monomer for water soluble acrylamide monomers to form hydrogels [58]. Three monomers were deposited sequentially onto the same position, with a solution containing a catalyst to initiate the reaction being printed subsequently.…”

Section: Microarray Formationmentioning

confidence: 99%

High throughput methods applied in biomaterial development and discovery

et al. 2010

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The high throughput discovery of new materials can be achieved by rapidly screening many different materials synthesised by a combinatorial approach to identify the optimal material that fulfils a particular biomedical application. Here we review the literature in this area and conclude that for polymers, this process is best achieved in a microarray format, which enable thousands of cell-material interactions to be monitored on a single chip. Polymer microarrays can be formed by printing pre-synthesised polymers or by printing monomers onto the chip where on-slide polymerisation is initiated.The surface properties of the material can be analysed and correlated to the biological performance using high throughput surface analysis, including time-of-flight secondary ion mass spectrometry (ToF-SIMS), Xray photoelectron spectroscopy (XPS) and water contact angle (WCA) measurements. This approach enables the surface properties responsible for the success of a material to be understood, which in turn provides the foundations of future material design. The high throughput discovery of materials using polymer microarrays has been explored for many cell-based applications including the isolation of specific 2 cells from heterogeneous populations, the attachment and differentiation of stem cells and the controlled transfection of cells.Further development of polymerisation techniques and high throughput biological assays amenable to the polymer microarray format will broaden the combinatorial space and biological phenomenon that polymer microarrays can explore, and increase their efficacy. This will, in turn, result in the discovery of optimised polymeric materials for many biomaterial applications.

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“…35 In addition, it has been used in the preparation of 1800 polymers on a glass slide by in situ nanoliter polymerization. 36,37 Because the already published approaches toward in situ polymerizations were designed for generating large numbers of different polymers in a format suitable for HT cellular screening (microarrays and grids), the recent research has been focused on using in situ polymerization for cellular patterning and enabling an increase in the library of synthetic materials suitable for cell patterning.…”

Section: Resultsmentioning

confidence: 99%

In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning

Liberski

Zhang

Bradley

2009

JALA: Journal of the Association for Laboratory Automation

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Drug-testing technologies, biosensor fabrication, tissue engineering, and basic biological research depend strongly on the patterning of live animal cells. Current techniques for controlling cellular adhesion are restricted with two primary limitations. Firstly, the complexity of the available patterns is very limited and, secondly, the pallet of materials that induce cellular patterning is exhaustible. Here, we demonstrate a method for computer-aided control of cell patterning using a scientific inkjet printer that yields a highly complex cellular pattern suitable for applications in regenerative medicine and rapid prototyping, and a strategy for using in situ polymerization for fabrication polymeric patterns directly on-chip.

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Inkjet fabrication of hydrogel microarrays using in situ nanolitre-scale polymerisation

Abstract: Polymer hydrogel microarrays were fabricated by inkjet printing of monomers and initiator, allowing up to 1800 individual polymer features to be printed on a single glass slide.

Cited by 63 publications

References 29 publications

Microarrays of over 2000 hydrogels – Identification of substrates for cellular trapping and thermally triggered release

Microarrays of over 2000 hydrogels – Identification of substrates for cellular trapping and thermally triggered release

High throughput methods applied in biomaterial development and discovery

In Situ Nanoliter-Scale Polymer Fabrication for Flexible Cell Patterning

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