Inkjet Printing for the Production of Protein Microarrays

McWilliam, Iain; Kwan, Marisa Chong; Hall, Duncan

doi:10.1007/978-1-61779-286-1_23

Cited by 32 publications

(21 citation statements)

References 24 publications

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“…The difficulty here is that we must anchor the swimmers down in a way that does not impede their spinning while still controlling their location. There are many different ways to place beads precisely on a substrate (including a number of methods that quite resemble inkjet printing [7]) However, it is easiest for us to use lithography to etch little wells into our substrate and use a magnetic force to hold down the magnetic microbeads.…”

Section: Anchoring Simmers To a Substratementioning

confidence: 99%

Engineering a Bacterial Flagella Forest for Sensing and Actuation – A Progress Report

Liu

Oti

et al. 2019

JoUR

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Flagella can be used to make magnetically-controlled microfluidic and nanoscale devices for biomedical applications in both vitro and vivo environments. They are capable of operating with high precision on the cellular and subcellular level. So far, scientists and engineers have successfully used monolithic inorganic materials or photoactive polymers [1] to mimic the helical bacterial flagella whose rotary-propulsion mechanism effectively overcomes the dominant viscous forces that prevail in a low Reynolds-number environment. Here, we focus on bacterial flagella and their rotary motion. The bacterial flagellum is an ideal biomaterial for constructing self-propelling nanoswimmers because it can reversibly change its geometry in response to different environmental stimuli such as pH, the local concentration of certain organic solvents, and mechanical force on the flagella. The bacterial flagellum is very easy to manipulate because it is composed of flagellin which can be mechanically isolated through vortexing and centrifugation, which enables flagella to be used as nanoscale sensors and mechanical transducers. Our project focuses on fabricating a bacterial flagella forest which consists of an ordered array of flagella on a glass substrate. Flagella are attached to magnetic nanobeads via biotin-avidin bonding for actuation by oscillating magnetic field.

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Section: Anchoring Simmers To a Substratementioning

confidence: 99%

Engineering a Bacterial Flagella Forest for Sensing and Actuation – A Progress Report

Liu

Oti

et al. 2019

JoUR

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“…This is achieved through monitoring their interaction with a library of well-defined molecular probes. A good history of the development of tools in this field is provided by McWilliam et al 2011. This details the history of progression from test tube scale to inkjet printing microarrays for a range of applications, such as the addition of nucleotides for in situ synthesis of nucleic acids (Schena et al 1998, Hughes et al 2001, Blanchard et al 1996, Goldmann et al 2000, Saaem et al 2010, with an example of an array created by inkjet printing shown in Figure 4a (Schena et al 1998).…”

Section: High Throughput 'System Discovery' Techniquesmentioning

confidence: 99%

Inkjet printing for pharmaceutics – A review of research and manufacturing

Daly

Harrington

Martin

et al. 2015

International Journal of Pharmaceutics

274

185

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Abstract:Global regulatory, manufacturing and consumer trends are driving a need for change in current pharmaceutical sector business models, with a specific focus on the inherently expensive research costs, high-risk capital-intensive scale-up and the traditional centralised batch manufacturing paradigm. New technologies, such as inkjet printing, are being explored to radically transform pharmaceutical production processing and the end-to-end supply chain. This review provides a brief summary of inkjet printing technologies and their current applications in manufacturing before examining the business context driving the exploration of inkjet printing in the pharmaceutical sector. We then examine the trends reported in the literature for pharmaceutical printing, followed by the scientific considerations and challenges facing the adoption of this technology. We demonstrate that research activities are highly diverse, targeting a broad range of pharmaceutical types and printing systems. To mitigate this complexity we show that by categorising findings in terms of targeted business models and Active Pharmaceutical Ingredient (API) chemistry we have a more coherent approach to comparing research findings and can drive efficient translation of a chosen drug to inkjet manufacturing.

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“…Inkjet printing is based on the ejection of drops from a nozzle, shot onto a surface. The generation of the drop is accomplished by piezoelectric micropumps, a continuous stream controlled by valves, or thermal inkjet technology, being the first one the most common jetting technique [ 71 ]. Non-contact printing can be applied to practically all substrates but is especially apt for damageable surfaces.…”

Section: Up-to-date Patterning Of Bresmentioning

confidence: 99%

Analytical Protein Microarrays: Advancements Towards Clinical Applications

Sauer

2017

Sensors

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Protein microarrays represent a powerful technology with the potential to serve as tools for the detection of a broad range of analytes in numerous applications such as diagnostics, drug development, food safety, and environmental monitoring. Key features of analytical protein microarrays include high throughput and relatively low costs due to minimal reagent consumption, multiplexing, fast kinetics and hence measurements, and the possibility of functional integration. So far, especially fundamental studies in molecular and cell biology have been conducted using protein microarrays, while the potential for clinical, notably point-of-care applications is not yet fully utilized. The question arises what features have to be implemented and what improvements have to be made in order to fully exploit the technology. In the past we have identified various obstacles that have to be overcome in order to promote protein microarray technology in the diagnostic field. Issues that need significant improvement to make the technology more attractive for the diagnostic market are for instance: too low sensitivity and deficiency in reproducibility, inadequate analysis time, lack of high-quality antibodies and validated reagents, lack of automation and portable instruments, and cost of instruments necessary for chip production and read-out. The scope of the paper at hand is to review approaches to solve these problems.

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Inkjet Printing for the Production of Protein Microarrays

Cited by 32 publications

References 24 publications

Engineering a Bacterial Flagella Forest for Sensing and Actuation – A Progress Report

Engineering a Bacterial Flagella Forest for Sensing and Actuation – A Progress Report

Inkjet printing for pharmaceutics – A review of research and manufacturing

Analytical Protein Microarrays: Advancements Towards Clinical Applications

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