Inmunodeficiencia común variable: caracterización clínica e inmunológica de pacientes y definición de subgrupos homogéneos con base en la tipificación de subpoblaciones de linfocitos B

Vélez, Augusto Quevedo; Castaño, Diana; Posada-Gómez, Rubén; Orrego, Julio César; Moncada, Marcela; Franco, José Luis

doi:10.7705/biomedica.v35i1.2401

Cited by 3 publications

(5 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Alteration in B cell subsets has been widely recognized to be present in PAD patients, although CVID patients are the more widely studied [ 9 , 14 , 23 , 24 ]. We found that the most frequently altered B cell subsets in PAD participants were the B memory compartment, especially switched memory B cells, and plasmablasts, even in non-CVID PAD patients, when compared to HD group ( Figure 3 ) indicating that these subsets are the most frequently altered, as previously reported in Colombian CVID patients [ 14 ]; however, no participant showed alterations neither in the frequency of Naïve CD5 + that had been associated with autoimmunity [ 20 ] nor the naïve CD5 − B cells, as reported in a high percentage of the Colombian CVID patients mentioned in the study above, and 4/9 patients showed less than 1% B cells, suggesting different alterations in B cell subsets between these two geographical areas of Colombia, although this comparison has to be taken with caution as different markers and gating strategies were used for the identification of B cell subsets. Moreover, alterations were found in other subsets such as transitional and unswitched memory B cells, especially when compared to the age-associated distribution of normal B cells ( Table 2 ), highlighting the importance of the comparison to healthy donors with a similar age to the patients.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, published data about immunological assessment in subjects diagnosed with PAD are limited in Colombia. The few studies available were published by the primary immunodeficiency group from Universidad de Antioquia [ 14 ]. For this reason, the aim of this study was to evaluate and describe the lymphocyte populations and B cell subsets frequency in patients seen in Cali, who came from different locations from the Colombian Southwest region.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

B Cell Subsets in Colombian Adults with Predominantly Antibody Deficiencies, Bronchiectasis or Recurrent Pneumonia

Giraldo-Ocampo

Bonelo

Zea-Vera

2022

ARM

View full text Add to dashboard Cite

Aim: To evaluate and describe lymphocyte populations’ and B cell subsets’ frequencies in patients presenting with Predominantly antibody deficiencies (PAD) and diagnosed with bronchiectasis or recurrent pneumonia seen in Cali (Colombian Southwest region). Materials and Methods: 16 subjects with PAD, 20 subjects with pulmonary complications (bronchiectasis or recurrent pneumonia) and 20 healthy donors (HD). Controls and probands between 14 and 64 years old, regardless of gender were included. Lymphocyte populations (T, B and NK cells) and B cell subsets were evaluated in peripheral blood mononuclear cells using flow cytometry, T/B/NK reagent and the pre-germinal center antibody panel proposed by the EUROflow consortium were used. EUROclass and the classification proposed by Driessen et al. were implemented. Results: CVID patients exhibited increase absolute numbers of CD8+ T cells and reduce NK cells as compare with HD, other PAD cases or pulmonary complications. PAD B cell subsets were disturbed when compared to the age range-matched healthy donors. Among B cell subsets, the memory B cell compartment was the most affected, especially switched memory B cells. Four participants were classified as B- and two CVID as smB-Trnorm and smB-21low groups according to EUROclass classification. The most frequent patterns proposed by Driessen et al. were B cell production and germinal center defect. Conclusions: B cell subsets, especially memory B cells, are disturbed in PAD patients from Southwestern Colombia. To the best of our knowledge this is the most comprehensive study of B cell subsets in Colombian adults.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

B Cell Subsets in Colombian Adults with Predominantly Antibody Deficiencies, Bronchiectasis or Recurrent Pneumonia

Giraldo-Ocampo

Bonelo

Zea-Vera

2022

ARM

View full text Add to dashboard Cite

show abstract

“…Alteration in B cell subsets has been recognized to be present in PAD patients, although CVID patients are the more widely studied [9,14,20,21]. It was found that the most frequently altered B cell subsets in PAD participants were the B memory compartment, especially Switched memory B cells, and plasmablasts, even in non-CVID PAD patients, when compared to the median of healthy donors ( gure 3) and to the age range-matched healthy donors (Table 2), indicating that this subsets are the most frequently altered as previously reported in Colombian CVID patients [14], however, no participant showed alterations neither in the frequency of Naïve CD5+ (also known as pre-naïve B cells) that had been associated with autoimmunity [22] nor the naïve CD5-B cells, as reported in a high percentage in the Colombian CVID patients mentioned above, and 4/9 patients showed less than 1% B cells, suggesting a different alterations in B cell subsets between these two geographical areas of Colombia, although the comparison has to be taken with precautious as different markers and gating strategy were used for the identi cation of B cell subsets. Furthermore, one CVD patient had no reduction in switched or unswitched memory B cells, which implies a different alteration pattern.…”

Section: Discussionmentioning

confidence: 99%

“…Currently, published data about immunological assessment in subjects diagnosed with PAD is limited in Colombia. The few studies available were published by the primary immunode ciency group from Universidad de Antioquia [14]. For this reason, the aim of this study was to evaluate the lymphocyte populations and B cell subsets in patients seen in Cali, who came from different locations from the Colombian Southwest region.…”

Section: Introductionmentioning

confidence: 99%

B Cell Subsets in Colombian Adults With Predominantly Antibody Deficiencies, Bronchiectasis or Recurrent Pneumonia

Giraldo-Ocampo

Perdomo

Zea-Vera

2021

Preprint

View full text Add to dashboard Cite

Purpose. To evaluate lymphocyte’s populations and B cell subsets in patients presenting with PAD diagnosed with bronchiectasis or recurrent pneumonia seen in Cali (Colombian Southwest region). Methods. 16 patients with PAD, 20 with bronchiectasis or recurrent pneumonia and 20 healthy controls were included. Lymphocyte populations (T, B & NK cells) and B cell subsets were evaluated in Peripheral Blood mononuclear cells (PBMC) using flow cytometry, using the T/B/NK reagent and the pre-germinal center antibody panel proposed by the EUROflow consortium, respectively. Clinical and sociodemographic data was collected (hospital records and interview). EUROclass and the classification proposed by Driessen et al were implemented. Results. B cell subsets were disturbed when compared to the age range matched healthy donors. Among B cell subsets,memory B cellcompartment was the most affected, especially switched memory B cells. Four participants were classified as B- and two CVID as smB-Trnorm and smB-21lo groups according to EUROclass classification. The most frequent pattern proposed by Driessen et al were B-cell production and germinal center defect. PAD participants also showed alteration in the B cell subsets correlation. Non-PAD participants with pulmonary complication also had alteration in B cell subsets.

show abstract

“…Para separar LB vírgenes y de memoria nosotros usamos el CD27, que en el caso de los LB es inducido después de exposición antigénica específica (Korniluk et al, 2014). El CD27 ha sido ampliamente utilizado para distinguir los LB entre vírgenes y de memoria tanto en trabajos experimentales como en el contexto clínico (Vélez et al, 2015). Sin embargo, Fecteau, Roy, y Néron, (2009) observaron que la expresión de CD27 disminuye en los primeros días luego de la estimulación con CD40L+IL-4 y a partir del día noveno se restaura, sugiriendo así que se modula luego de la activación de los LB.…”

Section: Discussionunclassified

Expresión diferencial de la proteína LRBA en los linfocitos B vírgenes y de memoria humanos

2020

View full text Add to dashboard Cite

La deficiencia de LRBA (Lipopolysaccharide-responsive and beige-like anchor protein), es una inmunodeficiencia primaria causada por una expresión defectuosa de la proteína intracelular LRBA, debido a mutaciones, homocigotas y heterocigotas compuestas, en el gen correspondiente. Los pacientes con esta inmunodeficiencia muestran un amplio espectro de manifestaciones clínicas que incluyen, principalmente, inmunodeficiencia y autoinmunidad. La deficiencia de LRBA se asocia con una disminución del recuento de linfocitos B (LB) de memoria con cambio de isotipo en sangre periférica y plasmablastos, y una respuesta defectuosa de anticuerpos específicos, así como un defecto en la apoptosis y la autofagia. En este estudio se evaluó la expresión diferencial intracelular de la proteína LRBA en los LB vírgenes y de memoria de sangre periférica humana, bajo diferentes condiciones por citometría de flujo. Evidenciamos que los LB de memoria presentan una mayor expresión de LRBA; por otra parte, demostramos que la expresión de esta proteína en los LB aumenta considerablemente en condiciones de activación; sin embargo, la magnitud del incremento en la expresión de LRBA bajo esta condición es mayor en los LB vírgenes. En contraste, evidenciamos que la expresión de esta proteína disminuye en LB sometidos a apoptosis, aunque aún en estas condiciones, es mayor en LB de memoria. Estos hallazgos sugieren que LRBA juega un papel importante en el desarrollo de la memoria inmunológica humoral.

show abstract

Inmunodeficiencia común variable: caracterización clínica e inmunológica de pacientes y definición de subgrupos homogéneos con base en la tipificación de subpoblaciones de linfocitos B

Cited by 3 publications

References 62 publications

B Cell Subsets in Colombian Adults with Predominantly Antibody Deficiencies, Bronchiectasis or Recurrent Pneumonia

B Cell Subsets in Colombian Adults with Predominantly Antibody Deficiencies, Bronchiectasis or Recurrent Pneumonia

B Cell Subsets in Colombian Adults With Predominantly Antibody Deficiencies, Bronchiectasis or Recurrent Pneumonia

Expresión diferencial de la proteína LRBA en los linfocitos B vírgenes y de memoria humanos

Contact Info

Product

Resources

About