Innate Immune Functions in hen Reproductive Organs

Yoshimura, Y.; Mageed, A. M. Abdel; Nii, Takahiro; Ariyadi, Bambang; Isobe, Naoki

doi:10.3184/175815514x13902927945697

Cited by 7 publications

(3 citation statements)

References 85 publications

(105 reference statements)

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“…Besides, an innate and adaptive immune were triggered during the SE challenge. We found that the TLR4 , NOD1 , AvβD7 , and IL‐1β mRNA were upregulated significantly in the ovary, which suggested the ovary, as immune niche, had a local immune environment to defend the birds against SE invasion (Yoshimura, Mageed, Nii, Ariyadi, & Isobe, ).…”

Section: Discussionmentioning

confidence: 96%

Comparative transcriptome analysis reveals PERP upregulated duringSalmonellaEnteritidis challenge in laying ducks

Zhang

Yang

et al. 2018

Journal Cellular Physiology

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Salmonella Enteritidis (SE) can be transmitted to eggs through cecum or the ovary from infected layers and causes food poisoning in humans. The mechanism of cecal transmission has been extensively studied. However, the mechanism and route of transovarian transmission of SE remain unclear. In this study, the ducks were orally inoculated with SE, and the ovarian follicles and stroma were collected to detect SE infection. The immune responses were triggered and the innate and adaptive immune genes (TLR4, NOD1, AvβD7, and IL‐1β) were upregulated significantly during the SE challenge. Moreover, the ovary tissues (small follicle and stroma) of susceptible and resistant–laying ducks were performed by RNA sequencing. We obtained and identified 23 differentially expressed genes (DEGs) between susceptible and resistant–laying ducks in both small follicle and stroma tissues ( p < 0.05). The DEGs were predominately identified in the p53 signaling pathway. The expression of key genes (p53, MDM2, PERP, caspase‐3, and Bcl‐2) involved in the signaling pathway was significantly higher in granulosa cells (dGCs) from SE‐infected ducks than those from uninfected ducks. Moreover, the overexpression of PERP resulted in further induction of p53, MDM2, caspase‐3, and Bcl‐2 during SE infection in dGCs. Whereas, an opposite trend was observed with the knockdown of PERP. Besides, it is further revealed that the PERP could enhance cell apoptosis, SE adhesion, and SE invasion in SE‐infected dGCs overexpression. Altogether, our results demonstrate the duck PERP involved in the ovarian local immune niche through p53 signaling pathway in dGCs challenged with SE.

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Section: Discussionmentioning

confidence: 96%

Comparative transcriptome analysis reveals PERP upregulated duringSalmonellaEnteritidis challenge in laying ducks

Zhang

Yang

et al. 2018

Journal Cellular Physiology

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“…The ability of reproductive tissues to synthesise AvBDs and their regulation in response to pathogens may play an essential role in innate immunity in the ovary and oviduct, establishing localised defenses within the host. Synthesised AvBDs notably fortify the defense mechanism of reproductive organs (Yoshimura et al 2014) and offer protection against infection (Yoshimura 2015).…”

Section: Introductionmentioning

confidence: 99%

Microbial Count and <i>AvBD10</i> Expressions in Ovaries and Oviducts of Kampung Unggul Balitbangtan (KUB)-1 Chickens Following Intravaginally CpG-ODN and <i>S</i>. Enteritidis

Suryohastari,

Sumarsono,

Giri-rachman

et al. 2024

J.Tropical Biodiversity Biotechnology

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Indonesia boasts diverse native chickens (Gallus gallus domesticus) known for more disease resistance in comparison to broiler chicken, and Kampung Unggul Balitbangtan (KUB)-1 is designated as Indonesia's superior breed. Salmonella Enteritidis (SE) is associated with salmonellosis, a foodborne illness that can be transmitted by transovarial, wherein colonisation in the oviduct ascends to the ovaries. However, studies mimicking transovarial salmonellosis via intravaginal treatment of chicken have been limited. Meanwhile, Cytosine-phosphate-guanine oligodeoxynucleotide (CpG-ODN) stimulation has been known to induce avian β-defensins (AvBDs). This in vivo study aimed to determine the effects of intravaginal CpG-ODN treatment and SE challenged on microbial count and AvBD10 expression regarding the potential of intravaginally CpG-ODN to enhance innate immunity as an alternative approach against transovarial Salmonellosis. A total of 39 KUB-1 chickens were divided into four groups: T1 (CpG-ODN treatment), T2 (SE treatment), T3 (CpG-ODN treatment + challenged with SE), and C (Control). Observation was carried out from day 1 to day 4 post-intravaginal (PI). We found a significant increase in ovarian microbial count (p≤0.05). Notably, ovaries and oviducts remained uncontaminated post-SE challenge. Intravaginal CpG-ODN treatment significantly upregulated AvBD10 in both ovaries (p=0.016) and oviducts (p=0.023). Therefore, KUB-1 chickens exhibit SE immunity, and intravaginal CpG-ODN administration holds promise for preventing transovarial Salmonellosis in laying hens.

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“…Toll-like receptors (TLRs) have emerged as a major component of the vertebrate pattern recognition receptor (PRR) repertoire, and can recognize conserved pathogen-associated molecular patterns (PAMPs) of bacteria, fungi, viruses and parasites as well as some self-ligands, such as host DNA in immune complexes and the malarial pigment hemozoin, (hemoglobin digested by malarial parasites), but not commensal microbes [ 1 - 3 ]. Upon activation by an agonist, TLRs induce the expression of a wide range of immunoregulatory and effector molecules [ 4 - 6 ], which take part in the regulation of immune cell maturation and the clearance of pathogenic microorganisms [ 5 , 7 - 9 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and functional characterization of Toll-like receptor 2–1 in geese

et al. 2015

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BackgroundToll-like receptor 2 (TLR2), an important pattern recognition receptor, activates proinflammatory pathways in response to various pathogens. It has been reported in humans and chicken, but not in geese, an important waterfowl species in China. Since some vaccines stimulate robust immune responsesl in chicken but not in geeeses we speculated that their immune systems are different.ResultsIn this study, we cloned the goose TLR2-1 gene using rapid amplification of cDNA ends (RACE)and showed that geese TLR2-1 encoded a 793-amino-acid protein, containing a signal secretion peptide, an extracellular leucine-rich repeat domain, a transmembrane domain and a Toll/interleukin-1 receptor signaling domain deduced from amino acid sequence. TLR2-1 shared 38.4%–93.5% homology with its homologues in other species. Tissue expression of geese TLR2-1 varied markedly, and was higher in kidney, cloacal bursa, skin and brain compared to other organs/tissues. HEK293 cells transfected with plasmids carrying goose TLR2-1 and NF-κB-luciferase responded significantly to stimulation with Mycoplasma fermentans lipopeptide. Furthermore, geese infected with Mycoplasma gallisepticum (MG) and Salmonella enteritidis (SE) showed significant upregulation of TLR2-1 in both in vivo and in vitro.ConclusionGeese TLR2-1 is a functional homologue of TLR2 present in other species and plays an important role in bacterial recognition in geese.

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Innate Immune Functions in hen Reproductive Organs

Cited by 7 publications

References 85 publications

Comparative transcriptome analysis reveals PERP upregulated duringSalmonellaEnteritidis challenge in laying ducks

Comparative transcriptome analysis reveals PERP upregulated duringSalmonellaEnteritidis challenge in laying ducks

Microbial Count and <i>AvBD10</i> Expressions in Ovaries and Oviducts of Kampung Unggul Balitbangtan (KUB)-1 Chickens Following Intravaginally CpG-ODN and <i>S</i>. Enteritidis

Identification and functional characterization of Toll-like receptor 2–1 in geese

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