Innate Nutritional Immunity

Núñez, Gabriel; Sakamoto, Kei; Soares, Miguel P.

doi:10.4049/jimmunol.1800325

Cited by 92 publications

(70 citation statements)

References 118 publications

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“…Such competition for iron also exists in vertebrate microbiota between beneficial, commensal, and pathogenic bacteria. In addition, competition for iron occurs as well between pathogens and hosts during infection: vertebrate immune systems have developed nutrient immunity defense mechanisms to protect them against invading pathogens by sequestering iron and consequently starving pathogens (1,2).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms

Perraud

Cantero

Roche

et al. 2020

Molecular & Cellular Proteomics

158

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Bacteria secrete siderophores to access iron, a key nutrient poorly bioavailable and the source of strong competition between microorganisms in most biotopes. Many bacteria also use siderophores produced by other microorganisms (exosiderophores) in a piracy strategy. Pseudomonas aeruginosa, an opportunistic pathogen, produces two siderophores, pyoverdine and pyochelin, and is also able to use a panel of exosiderophores. We first investigated expression of the various iron-uptake pathways of P. aeruginosa in three different growth media using proteomic and RT-qPCR approaches and observed three different phenotypic patterns, indicating complex phenotypic plasticity in the expression of the various iron-uptake pathways. We then investigated the phenotypic plasticity of iron-uptake pathway expression in the presence of various exosiderophores (present individually or as a mixture) under planktonic growth conditions, as well as in an epithelial cell infection assay. In all growth conditions tested, catechol-type exosiderophores were clearly more efficient in inducing the expression of their corresponding transporters than the others, showing that bacteria opt for the use of catechol siderophores to access iron when they are present in the environment. In parallel, expression of the proteins of the pyochelin pathway was significantly repressed under most conditions tested, as well as that of proteins of the pyoverdine pathway, but to a lesser extent. There was no effect on the expression of the heme and ferrous uptake pathways. Overall, these data provide precise insights on how P. aeruginosa adjusts the expression of its various iron-uptake pathways (phenotypic plasticity and switching) to match varying levels of iron and competition.

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Section: Introductionmentioning

confidence: 99%

Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms

Perraud

Cantero

Roche

et al. 2020

Molecular & Cellular Proteomics

158

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“…Two candidates were potentially involved in metal binding, NGO1215 (AccA [86]), and NGO1701 (hypothetical). Metal transport membrane proteins are important for bacterial metabolism, growth and pathogenesis by providing host nutritional immunity escape strategies [98]. Iron requirements and the proteins associated with iron transport and metabolism in Neisseriae are well studied [99,100]; zinc, copper and other metals are also critical [101] along with proteins that modulate their functions, such as ZnuD, TdTs and CopA [102][103][104][105] to mention a few.…”

Section: Discussionmentioning

confidence: 99%

Integrated Bioinformatic Analyses and Immune Characterization of New Neisseria gonorrhoeae Vaccine Antigens Expressed during Natural Mucosal Infection

et al. 2019

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There is an increasingly severe trend of antibiotic-resistant Neisseria gonorrhoeae strains worldwide and new therapeutic strategies are needed against this sexually-transmitted pathogen. Despite the urgency, progress towards a gonococcal vaccine has been slowed by a scarcity of suitable antigens, lack of correlates of protection in humans and limited animal models of infection. N. gonorrhoeae gene expression levels in the natural human host does not reflect expression in vitro, further complicating in vitro-basedvaccine analysis platforms. We designed a novel candidate antigen selection strategy (CASS), based on a reverse vaccinology-like approach coupled with bioinformatics. We utilized the CASS to mine gonococcal proteins expressed during human mucosal infection, reported in our previous studies, and focused on a large pool of hypothetical proteins as an untapped source of potential new antigens. Via two discovery and analysis phases (DAP), we identified 36 targets predicted to be immunogenic, membrane-associated proteins conserved in N. gonorrhoeae and suitable for recombinant expression. Six initial candidates were produced and used to immunize mice. Characterization of the immune responses indicated cross-reactive antibodies and serum bactericidal activity against different N. gonorrhoeae strains. These results support the CASS as a tool for the discovery of new vaccine candidates.

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“…Iron is essential for most organisms because it serves as an electron donor and acceptor in various metabolic processes. Therefore, iron holds a central position in host-pathogen interactions [12]. While hosts evolved mechanisms to deprive pathogens of iron, pathogens coevolved mechanisms to acquire the host's iron.…”

Section: Nutritional Immunity and Iron Metabolism In Ticksmentioning

confidence: 99%

“…While hosts evolved mechanisms to deprive pathogens of iron, pathogens coevolved mechanisms to acquire the host's iron. Host mechanisms involved in limiting the access of pathogens to iron were termed 'nutritional immunity' [12]. During feeding, ticks are exposed to the large amounts of iron present in the blood meal.…”

Section: Nutritional Immunity and Iron Metabolism In Ticksmentioning

confidence: 99%

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Tick–Pathogen Interactions: The Metabolic Perspective

Cabezas-Cruz

Espinosa

Alberdi

et al. 2019

Trends in Parasitology

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The first tick genome published in 2016 provided an invaluable tool for studying the molecular basis of tick-pathogen interactions. Metabolism is a key element in host-pathogen interactions. However, our knowledge of tick-pathogen metabolic interactions is very limited. Recently, a systems biology approach, using omics datasets, has revealed that tick-borne pathogen infection induces transcriptional reprograming affecting several metabolic pathways in ticks, facilitating infection, multiplication, and transmission. Results suggest that the response of tick cells to tick-borne pathogens is associated with tolerance to infection. Here we review our current understanding of the modulation of tick metabolism by tick-borne pathogens, with a focus on the model intracellular bacterium Anaplasma phagocytophilum. Tick-Pathogen Metabolic Interactions at the Center of the Infectious Storm Metabolism is key to cellular function [1,2]. In consequence, all major molecular pathways in living cells are interconnected to, and regulated by, the availability and levels of metabolites [1,2]. Recent studies in mammalian cells showed that interactions between intracellular bacterial pathogens and the host cells can lead to physiological changes in both interacting members [3]. The metabolic adaptations in mammalian cell-pathogen systems promote proliferation or elimination of the pathogen within the host cells [3]. Tick-pathogen interactions, however, involve a more complex array of outcomes which include conflict and cooperation that ultimately benefit both ticks and pathogens [4]. This is not surprising considering that, in vector-borne pathogen systems, the survival of the vector is essential for the completion of the life cycle of the pathogen. In addition, tick-borne pathogen infection increases tick performance in challenging environmental conditions [5,6]. Therefore, in some cases, vector tolerance to pathogen infection is an advantageous life trait in arthropod vectors [7]. Modulation of tick metabolism by tick-borne pathogens is a result of coevolution and adaption to a considerable number of tick and reservoir host species [4,8,9]. The Ixodes scapularis genome, the first for a medically important chelicerate, is the only tick genome available so far [10]. The sequencing of the I. scapularis genome was an essential step towards the understanding of the molecular processes that support the parasitic lifestyle of the tick and its success as a vector of multiple pathogens, including bacteria, viruses, protozoa, and helminths, which constitute a growing burden for human and animal health worldwide [11]. This review focuses on recent research that provided insights on the finely tuned metabolic changes that the model pathogen A. phagocytophilum induces in its tick vector I. scapularis. For comparative purposes, tick metabolic changes induced by other pathogens, including mainly Borrelia spp., flaviviruses, and fungi, will be also included (Table 1). Results suggest that tickborne pathogens use tick metabolism as a hub to modulate ...

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Innate Nutritional Immunity

Cited by 92 publications

References 118 publications

Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms

Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms

Integrated Bioinformatic Analyses and Immune Characterization of New Neisseria gonorrhoeae Vaccine Antigens Expressed during Natural Mucosal Infection

Tick–Pathogen Interactions: The Metabolic Perspective

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