Inner component of respiratory syncytial virus

Nucleic acids of respiratory syncytial virus

“…0.5 ,ug of actinomycin D (Sigma) was added for 1 h at 360C, after which the medium was replaced with drugfree MEM containing 3% fetal bovine serum. L_3Hamino acids (10 PCi/ml of MEM containing 10% normal complement of L-amino acids) or [5-3H]uridine (10 ltCi/mil) was added at this time for the production of labeled virus. Under these conditions, syncytia were usually visible after 24 h although the infection was usually allowed to proceed for 48 to 72 h when syncytium formation was complete.…”

“…The virion resembles the paramyxoviruses in gross physical appearance as demonstrated by electron microscopy (1,3,15), but it differs from this group of viruses in several important aspects. RS virus lacks a demonstrable hemagglutinating or neuraminidase activity (6,22), and its nucleocapsid differs from that of the myxoviruses and paramyxoviruses in diameter and in helical pitch (10,26). The proper classification of this virus is still uncertain.…”

Morphogenesis and Ultrastructure of Respiratory Syncytial Virus

“…Studies on the morphogenesis of these viruses have revealed round or filamentous particles which mature by a budding process at the surface of infected cells. Attempts to discern structural features of RS virus and its components by the negativestaining technique have been handicapped by the extreme fragility of the virion (7,11). The experiments reported here represent an effort at further characterization of RS virus with immunoferritin techniques and a comparison of its appearance in negatively stained, thin-sectioned, and freeze-etched preparations.…”

Isolation, characterization, and pathogenicity studies of a bovine respiratory syncytial virus

“…Early studies of the ultrastructure of the virus produced both agreeing and conflicting results (1,6,21,38). Host investigators agree that the morphogenesis of RS virus resembles that of other myxo vi ruses.…”

“…Since the diameter of the nucleocapsid seems to be a critical factor in classifying the RS virus, measurements have been made from many preparations with a conflicting range of 12-18 nm diameter having been reported (6,7,21,38). It is felt that the extreme fragility of the virus plus different methods of fixation are the major causes for this discrepancy, it appears that with meticulous efforts taken in preparation of specimens for examination, a quite constant average diameter is 13 nm (54).…”