Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (<i>Vicugna Pacos</i>)

Liguori, Giovanna; Paino, Salvatore; Squillacioti, Caterina; Luca, Arianna De; Alì, Sabrina; Langella, Emilia; Mirabella, Nicola

doi:10.4081/ijas.2013.e15

Cited by 6 publications

(7 citation statements)

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“…All subjects were lacking of preputial adhesions which is considered a signal of sexual maturity (Tibary & Vaughan, 2006). Tissue collection was performed as described by Liguori et al (2013). After removal, the testes were finely cut in small samples which were either fixed in Bouin's fluid for immunohistochemistry as described in details elsewhere (Squillacioti, De Luca, Paino, Langella, & Mirabella, 2009) or frozen in liquid nitrogen and stored at −80°C until used for Western blotting analysis.…”

Section: Animalsmentioning

confidence: 99%

Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Liguori

Squillacioti

Assisi

et al. 2017

Reprod Domestic Animals

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Contents The orexins A (OxA) and B (OxB) are two hypothalamic peptides involved in many physiological functions of the mammalian body. They act through the binding of two G‐coupled receptors named receptor 1 (OX1) and receptor 2 (OX2) for orexins. The first receptor is specific for OxA, while the second binds both the substances with equal affinity. The orexins and the relative receptors have been traced by means of different techniques also at the periphery of the body and particularly in the adrenals, and in gastrointestinal and genital organs. Aim of this work was to investigate the presence of OxB and OX2 by means of immunohistochemistry and Western blotting analysis in the testis of the South American camelid alpaca, a species primarily breed in Chile and Ecuador and recently diffused in Europe where the quality of its wool is particularly appreciated. OxB immunoreactivity (IR) was found in the tubular compartment of the testis where spermatogonia (resting), zygotene and pachytene spermatocytes, and spermatids clearly showed differently sized and shaped cytoplasmic positive structures. OX2‐IR was found both in the interstitial and tubular compartments of the testis and particularly in Leydig cells and round and elongated spermatids. Western blotting analysis of testis lysates showed the presence of a protein band whose molecular weight corresponded to that currently assigned to OX2. Such findings easily translate the hypothesis that OxB and its receptor 2 play a functional role both in the interstitial and tubular compartments of the alpaca testis.

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Section: Animalsmentioning

confidence: 99%

Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Liguori

Squillacioti

Assisi

et al. 2017

Reprod Domestic Animals

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“…Similarly, neuronal fibers in the epididymis have been localized within the perimuscular coat, subepithelial regions, and in the coat of vessels within the interstitium. [ 16 17 ] Nerve distribution pattern in the epididymis has been found to be segment-specific with the cauda segment having the highest nerve ramifications owing to its thick muscular wall. [ 18 ] The neurotransmitters in the epididymal nerves have been suggested to be important in mediating epididymal epithelial cell function of electrolyte transport and protein processing.…”

Section: Introductionmentioning

confidence: 99%

Nerve and Glial Cell Expressions in the Testes and Epididymides of Different Age Groups of Cane Rat (Thryonomys swinderianus)

Omirinde

Olukole

Oke

2021

Journal of Microscopy and Ultrastructure

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Purpose: This study was conducted to examine the variations in the expressions of neuronal and glial cell markers in the testes and epididymides of different age groups of cane rat using histochemical and immunohistochemical techniques. Method: Thirty (32) healthy domesticated male cane rats were used for this investigation. The rats were divided into four groups (prepubertal [≤4 months], pubertal (>4 ≤12 months), adult (>12 ≤30 months), and aged (>30 months)] of 8 animals each. Subsequent to anesthesia and intracardiac perfusion of the rats with 10% buffered formalin, testes were harvested and preliminary assessment of nervous and glial structures was determined using the Golgi technique. Specific immunolocalization was done using the anti-neurofilament (NF-20) and anti-glial fibrillary acid protein (GFAP) for the expressions of neuronal and astrocyte-like cells, respectively. Result: Neuronal and astrocyte-like structures as revealed by the Golgi procedure were demonstrated in the tunica albuginea and interstitium of the testes as well as in the periductal muscle coat and epididymal interstitium of the caput down to the caudal segments. Golgi signal intensities of the expressions in both testes and epididymides increased with age advancement. Immunolocalization of the nerve structures and glial cells tallied with the Golgi results. However, NF signal intensity was significantly higher in the adult relative to others. Similarly, GFAP signal intensity increased with age increment. Conclusion: This study has shown that the variation in the expression of neuronal and glial cells in the testis and epididymis of the cane rat could be associated with increased reproductive reproductive activity.

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“…After rehydration, the antigen retrieval method was applied to the sections as previously described by Liguori et al (2017) [ 36 ]. The immunohistochemical method performed for the present research was the avidin–biotin–peroxidase complex (ABC) and the protocol used was that previously described by Liguori et al (2013) and De Luca et al (2014) [ 40 , 41 ]. The sections were covered with mouse monoclonal anti-OxA and rabbit polyclonal anti-OX1R primary antibodies, diluted 1:200, applied on sections overnight at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs

et al. 2018

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BackgroundCryptorchidism is one of the most common birth disorders of the male reproductive system identified in dogs and other mammals. This condition is characterised by the absence of one (unilateral) or both (bilateral) gonads from the scrotum. The peptides orexin A (OxA) and B (OxB) were obtained by post-transcriptional proteolytic cleavage of a precursor molecule, called prepro-orexin. These substances bind two types of G-coupled receptors called receptor 1 (OX1R) and 2 (OX2R) for orexins. OX1R is specific to OxA while OX2R binds the two peptides with equal affinity. Orexins modulate a great variety of body functions, such as the reproductive mechanism. The purpose of the present research was to study the presence of OxA and its receptor 1 and their possible involvement in the canine testis under healthy and pathological conditions.MethodsThis study was performed using adult male normal dogs and male dogs affected by unilateral cryptorchidism. Tissue samples were collected from testes and were divided into three groups: normal, contralateral and cryptic. The samples were used for immunohistochemistry, Western blot and in vitro tests for testosterone evaluation in normal and pathological conditions.ResultsOxA-immunoreactivity (IR) was described in interstitial Leydig cells of the normal gonad, and Leydig, Sertoli cells and gonocytes in the cryptic gonad. In the normal testis, OX1R-IR was described in Leydig cells, in pachytene and second spermatocytes and in immature and mature spermatids throughout the stages of the germ developing cycle of the male gonad. In the cryptic testis OX1R-IR was distributed in Leydig and Sertoli cells. The presence of prepro-orexin and OX1R was demonstrated by Western blot analysis. The incubation of fresh testis slices with OxA caused the stimulation of testosterone synthesis in the normal and cryptic gonad while the steroidogenic OxA-induced effect was cancelled by adding the selective OX1R antagonist SB-408124.ConclusionsThese results led us to hypothesise that OxA binding OX1R might be involved in the modulation of spermatogenesis and steroidogenesis in canine testis in healthy and pathological conditions.

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Innervation and Immunohistochemical Characteristics of Epididymis in Alpaca Camelid (Vicugna Pacos)

Cited by 6 publications

References 60 publications

Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)

Nerve and Glial Cell Expressions in the Testes and Epididymides of Different Age Groups of Cane Rat (Thryonomys swinderianus)

Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs

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