Innovated formulation of TCM pangolin scales to develop a nova therapy of rheumatoid arthritis

Lv, Huiqing; Xie, Zhihua; Hu, Xuanming; Li, Haichang; Sun, Jing; Chen, Xuezhi; Wen, Chengping

doi:10.1016/j.biopha.2020.109872

Cited by 15 publications

(8 citation statements)

References 38 publications

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“…Emerging evidence has indicated that traditional Chinese medicine has a positive effect on RA. 24 25 26 In this study, we investigated the effect of CAT from traditional Chinese medicine on Th17 cells differentiation. We first investigated the effect of CAT on murine CIA in CIA mice and found that Th17 cells and levels of the inflammatory cytokines IL-2, IL-6, IL-21, and IL-23 were markedly decreased after CAT treatment, suggesting that CAT might negatively regulate Th17 cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Zhang

Chen

et al. 2022

Yonsei Med J

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Purpose Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease, and Th17 cells are key factors in the pathogenesis of human inflammatory conditions, such as RA. Catalpol (CAT), a component in Rehmanniae Radix (RR), has been found to regulate human immunity. However, the effects of CAT on Th17 cell differentiation and improvement of RA are not clear. Materials and Methods Collagen-induced arthritis (CIA) mice were constructed to detect the effects of CAT on arthritis and Th17 cells. The effect of CAT on Th17 differentiation was evaluated with let-7g-5p transfection experiments. Flow cytometry was used to detect the proportion of Th17 cells after CAT treatment. Levels of interleukin-17 and RORγt were assessed by qRT-PCR and enzyme-linked immunosorbent assay. The expression of signal transducer and activator of transcription 3 (STAT3) was determined by qRT-PCR and Western blot. Results We found that the proportion of Th17 cells was negatively associated with let-7g-5p expression in CIA mice. In in vitro experiments, CAT suppressed traditional differentiation of Th17 cells. Simultaneously, CAT significantly decreased Tregs-to-Th17 cells transdifferentiation. Our results demonstrated that CAT inhibited Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p and that the suppressive effect of CAT on traditional differentiation of Th17 cells is not related with let-7-5p. Conclusion Our data indicate that CAT may be a potential modulator of Tregs-to-Th17 cells transdifferentiation by up-regulating let-7g-5p to reduce the expression of STAT3. These results provide new directions for research into RA treatment.

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Section: Discussionmentioning

confidence: 99%

Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Zhang

Chen

et al. 2022

Yonsei Med J

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“…The major factor influencing pangolin demand is a culture steeped in traditional ethnomedicine use; high return for profit, high global demand for TCM, and decreases of Chinese pangolin populations (Anonymous 1938;Wu and Ma 2007;van Uhm 2016;Lv et al 2020). The limited stocks and quota set by the authority which fall below the required stock to meet the TCM manufacturer and local demands has resulted in the proliferation of the illegal pangolin trade (Yin et al 2015;van Uhm 2016;Wong 2019;van Uhm and Wong 2019).…”

Section: Pangolin Smuggling Routes and Hotspotsmentioning

confidence: 99%

Dissecting the illegal pangolin trade in China: An insight from seizures data reports

Omifolaji¹,

Hughes²,

Ibrahim³

et al. 2022

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Wildlife trafficking poses a major threat to global biodiversity. Species such as pangolins are particularly vulnerable and trade continues almost unabated despite numerous interventions aimed at eradicating illegal wildlife trade. Despite restrictions on the pangolin trade, thousands of pangolins continue to be intercepted annually. We focused on China because of the recent delisting of pangolins from the Chinese pharmacopeia, and their removal from healthcare insurance, despite deeply ingrained traditions of having pangolins for ethno-medicinal use. We collated pangolin interception data from public online media seizure reports to characterize the pangolin trade within China, and found that a total of 326 independent seizures equivalent to 143,130 pangolins (31,676 individuals and 222,908 kg of scale) were reported in 26 provinces. Pangolin domestic seizures are greatest in the southern cities of Dehong, Fangchenggang, and Guangzhou. Also, we found 17 countries within the global pangolins range which were the major source of the pangolin shipments to China. The number of arrests and convictions was much lower than the number of pangolin incidents reported. Our results show a significant increase in the volume of scales and number of live pangolin seizures after amended endangered species law came into effect in 2018, and recorded the highest number of individual pangolin interceptions. China has shown increasing wildlife seizures over time, owing partly to emergent trends in the international wildlife trade as well as increasing global demand for ethnomedicine. The future eradication of illegal wildlife trade in China is dependent not only on stringent border control and offender prosecution but also the; removal of other threatened species from the pharmacopeia and healthcare insurance which includes wildlife derivatives. Furthermore, our work highlights importance of current policy intervention to combat the pangolin trade within China, and the need for further interventions both within China and in export countries.

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“…Therefore, investigating more practical treatments for RA is highly necessary. For this reason, an increasing number of researchers have turned their attention to traditional Chinese medicine, such as medicine formulations 9,10 . Astragalus membranaceus (Fisch.)…”

Section: Introductionmentioning

confidence: 99%

Astragalosides inhibit proliferation of fibroblast‐like synoviocytes in experimental arthritis by modulating LncRNA S56464.1/miR‐152‐3p/Wnt1 signaling axis

et al. 2023

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Aim Astragalus membranaceus (Fisch.) Bunge., the dried root of the plant A. membranaceus, is widely used in the treatment of rheumatoid arthritis (RA) in many Chinese herbal remedies. Astragalosides (AST) is the primary medicinal ingredient of A. membranaceus and has a therapeutic effect on RA, but the specific mechanism of this effect has yet to be elucidated. Methods In this study, MTT and flow cytometry were used to determine the effects of AST on fibroblast‐like synoviocyte (FLS) proliferation and cell cycle progression. Additionally, real‐time quantitative polymerase chain reaction and Western blotting were used to determine the effects of AST on the LncRNA S56464.1/miR‐152‐3p/Wnt1 signaling axis and on critical genes that are essential to the Wnt pathway. Results The data showed that after the administration of AST, FLS proliferation and LncRNA S56464.1, β‐catenin, C‐myc, Cyclin D1, and p‐GSK‐3β(Ser9)/GSK‐3β expression were significantly reduced, and miR‐152 and SFRP4 expression was notably increased. Conclusion These results suggest that AST can inhibit FLS proliferation by modulating the LncRNA S56464.1/miR‐152‐3p/Wnt1 signaling axis and that AST may be a potential therapeutic drug for RA.

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Innovated formulation of TCM pangolin scales to develop a nova therapy of rheumatoid arthritis

Cited by 15 publications

References 38 publications

Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Dissecting the illegal pangolin trade in China: An insight from seizures data reports

Astragalosides inhibit proliferation of fibroblast‐like synoviocytes in experimental arthritis by modulating LncRNA S56464.1/miR‐152‐3p/Wnt1 signaling axis

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Innovated formulation of TCM pangolin scales to develop a nova therapy of rheumatoid arthritis

Cited by 15 publications

References 38 publications

Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

Catalpol Inhibits Tregs-to-Th17 Cell Transdifferentiation by Up-Regulating Let-7g-5p to Reduce STAT3 Protein Levels

﻿Dissecting the illegal pangolin trade in China: An insight from seizures data reports

Astragalosides inhibit proliferation of fibroblast‐like synoviocytes in experimental arthritis by modulating LncRNA S56464.1/miR‐152‐3p/Wnt1 signaling axis

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Dissecting the illegal pangolin trade in China: An insight from seizures data reports