The article is dedicated to examining the role of startups in creating innovative ecosystems. The purpose of the study is to identify key indicators that reflect the importance of AI startups in the formation of these ecosystems. The research methodology encompasses a diverse array of analytical tools and approaches to provide a comprehensive understanding of the subject. It includes statistical analysis for quantifying and interpreting data related to startup activities and their impacts on innovation ecosystems. Additionally, the study incorporates general scientific approaches, which involve systematic inquiry, and theoretical framework development, to ensure a robust and well-rounded investigation of the role of startups in creating innovative ecosystems. The findings reveal that innovative ecosystems are networks of interdependent actors that drive entrepreneurship within a specific territory. There has been a significant global increase in such ecosystems, with a projection to reach 155 by 2025. Today, about 3% of companies use artificial intelligence technologies, and it is predicted that by 2030, 80% of companies will have adopted artificial intelligence technologies to some extent, and the global impact of AI on GDP will be $16 trillion. Startups are identified as crucial in these ecosystems, often leading their development, especially in countries like the United States, the UK, Israel, Canada, and Sweden. The global startup landscape shows fluctuations with sectors like life sciences and Deep Tech demonstrating resilience and growth, highlighting the essential role of AI startups in fostering innovation and economic development. The research concludes that startups are moving innovation ecosystems away from traditional corporate-driven models to more dynamic, agile, and open forms of innovation. They bring rapid, disruptive changes to markets, diversify funding sources, and create job opportunities, with big companies increasingly collaborating with startups for new ideas and technologies. The practical significance of this study lies in providing insights for policymakers, entrepreneurs, and investors on fostering and leveraging startups for economic growth and innovation. It highlights the need for supportive environments that encourage startup activities, particularly in emerging economies.