Innovation in Newcastle Disease Virus Vectored Avian Influenza Vaccines

Kim, Shin-Hee; Samal, Siba K.

doi:10.3390/v11030300

Cited by 33 publications

(34 citation statements)

References 66 publications

(77 reference statements)

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“…Thus, it is necessary to develop a novel bivalent vaccine protect against NDV and IBV. In recent decades, attempts have been made to develop recombinant vaccines using NDV [48][49][50], adenovirus [51], duck enteritis virus [52], and avian metapneumovirus [53] viral backbones expressing the S1 or S2 portion of the IBV spike gene with differing levels of protection from IBV challenge. Among them, the NDV vector shows great promise due to its excellent characteristics, such as rapid and long-lasting immune response, low production costs, convenience of administration, and genetic stability [45].…”

Section: Discussionmentioning

confidence: 99%

“…Among them, the NDV vector shows great promise due to its excellent characteristics, such as rapid and long-lasting immune response, low production costs, convenience of administration, and genetic stability [45]. The avirulent NDV strain LaSota is an ideal vector that has been approved for use as a vaccine and is widely used worldwide [49]. It should be noted that the maternally derived antibodies of one-day-old chicks probably interfere with LaSota vaccine immunity.…”

Section: Discussionmentioning

confidence: 99%

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A Recombinant La Sota Vaccine Strain Expressing Multiple Epitopes of Infectious Bronchitis Virus (IBV) Protects Specific Pathogen-Free (SPF) Chickens against IBV and NDV Challenges

et al. 2019

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Infectious bronchitis (IB) and Newcastle disease (ND) are two major infectious diseases that are a threat to the domestic poultry industry. In this study, we successfully generated a recombinant LaSota candidate vaccine strain, rNDV-IBV-T/B, which expresses a short, synthetic, previously identified IBV S1 multi-epitope cassette using the reverse genetic system. The recombinant virus was propagated in nine-day-old embryonated chicken eggs for 20 passages and genetic stability was confirmed by whole genome DNA sequencing. The recombinant virus had a hemagglutination (HA) titer of 2 10 , mean death time (MDT) of 118 hours, and intracerebral pathogenicity index (ICPI) of 0.05. None of these were significantly different from the parental Newcastle disease virus (NDV) LaSota strain (p > 0.05). Vaccination of white leghorn chickens at one day of age with 10 6 EID 50 rNDV-IBV-T/B provided 90% protection against virulent IBV M41 challenge at three weeks of age, which was significantly higher than the protection of the control group vaccinated with phosphate-buffered saline (PBS) (p < 0.05). The ciliostasis scores of rNDV-IBV-T/B-vaccinated and LaSota-vaccinated groups were 4.2 and 37.6, respectively, which indicated that rNDV-IBV-T/B vaccination reduced the pathogenicity of IBV toward the trachea. Furthermore, real-time RT-PCR assay showed that the rNDV-IBV-T/B vaccination resulted in low levels of viral load (647.80 ± 49.65 RNA copies) in the trachea four days post-challenge, which is significantly lower than groups vaccinated with PBS (8591.25 ± 311.10 RNA copies) or LaSota (7742.60 ± 298.50 RNA copies) (p < 0.05). Meanwhile, the same dose of rNDV-IBV-T/B vaccination provided complete protection against velogenic NDV F48E9 challenge. These results demonstrate that the rNDV-IBV-T/B strain is a promising vaccine candidate to control both IB and ND simultaneously. Furthermore, epitope-based live vector vaccines provide an alternative strategy for the development of cost-effective and, broadly, cross-protective vaccines.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Recombinant La Sota Vaccine Strain Expressing Multiple Epitopes of Infectious Bronchitis Virus (IBV) Protects Specific Pathogen-Free (SPF) Chickens against IBV and NDV Challenges

et al. 2019

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“…Hence, a stronger immune response to HA is achieved when rAPMV-3/HA is used for prime immunization followed by boosting immunization at 3-week of age with rNDV expressing HA. Overcome of pre-existing antibodies has also been reported for other paramyxovirus vectors [37][38][39] . Furthermore, these results indicate that the rAPMV-3/HA has high efficiency replication in a greater range of chick organs.…”

Section: Figurementioning

confidence: 79%

A recombinant avian paramyxovirus serotype 3 expressing the hemagglutinin protein protects chickens against H5N1 highly pathogenic avian influenza virus challenge

Shirvani

Varghese

Paldurai

et al. 2020

Sci Rep

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Highly pathogenic avian influenza (HPAI) is a devastating disease of poultry and a serious threat to public health. Vaccination with inactivated virus vaccines has been applied for several years as one of the major policies to control highly pathogenic avian influenza virus (HPAIV) infections in chickens. Viral-vectored HA protein vaccines are a desirable alternative for inactivated vaccines. However, each viral vector possesses its own advantages and disadvantages for the development of a HA-based vaccine against HPAIV. Recombinant Newcastle disease virus (rNDV) strain LaSota expressing HA protein vaccine has shown promising results against HpAiV; however, its replication is restricted only to the respiratory tract. Therefore, we thought to evaluate avian paramyxovirus serotype 3 (APMV-3) strain Netherlands as a safe vaccine vector against HPAIV, which has high efficiency replication in a greater range of host organs. In this study, we generated rAPMV-3 expressing the HA protein of H5N1 HPAIV using reverse genetics and evaluated the induction of neutralizing antibodies and protection by rAPMV3 and rNDV expressing the HA protein against HPAIV challenge in chickens. Our results showed that immunization of chickens with rAPMV-3 or rNDV expressing HA protein provided complete protection against HPAIV challenge. However, immunization of chickens with rAPMV-3 expressing HA protein induced higher level of neutralizing antibodies compared to that of rNDV expressing HA protein. These results suggest that a rAPMV-3 expressing HA protein might be a better vaccine for mass-vaccination of commercial chickens in field conditions. Avian influenza viruses (AIVs) are members of the genus Alphainfluenzavirus in the family Orthomyxoviridae 1 . They can cause a wide range of clinical disease in chickens. Alphainfluenzaviruses are classified into combinations of 18 H (H1-H18) and 11 N (N1-N11) subtypes, based on antigenic differences of their hemagglutinin (HA) and neuraminidase (NA) surface glycoproteins. H1-H16 and N1-N9 subtypes have been found in avian species 2 . AIVs are divided into low pathogenic avian influenza viruses (LPAIVs) that cause restricted respiratory and/or intestinal disease without mortality in specific pathogen free (SPF) chickens, and highly pathogenic avian influenza viruses (HPAIVs) that cause high mortality following an acute systemic infection in SPF chickens 3,4 . Avian influenza is a devastating disease of poultry and a serious threat to public health, which is caused by H5 or H7 subtype of HPAIV 5,6 .Nationwide vaccination with inactivated virus vaccines and/or viral vectored HA-based vaccines is one of the major policies that is currently implemented against HPAIV in chickens in many countries 7 . Although the greater part of currently used vaccines are inactivated vaccines, this type of vaccine is not the best choice to combat HPAIV infection in poultry. Because not only the effectiveness of these vaccines is suboptimal, but also the processes of production and administration of these vaccines are ex...

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“…3 Newcastle disease (ND) and avian influenza (AI) of the H5N1 subtype have been recognized as the most important respiratory tract infection pathogens that have significantly threatened the poultry sector in Egypt. 4,5 In recent years, poultry industry faced severe outbreaks due to mixed infections of these two viruses which were causing high mortality and great economic losses. 4,5 Both viruses are contagious and widespread avian disease affecting most species of birds.…”

Section: Introductionmentioning

confidence: 99%

“…4,5 In recent years, poultry industry faced severe outbreaks due to mixed infections of these two viruses which were causing high mortality and great economic losses. 4,5 Both viruses are contagious and widespread avian disease affecting most species of birds. The ND affected birds exhibit the symptoms of irregularities of respiratory, nervous and digestive system.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Immune Boosting Properties and Combating of Multiple Respiratory Viral Infections by fifteen Euphorbiaceae Plant Extracts

Abd‐Alla¹,

Sweelam²,

El‐Kashak³

et al. 2019

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Objective: Fifteen Euphorbiaceae plant extracts were evaluated for their antiviral and immune boosting properties. Methods: The maximum non-toxic concentration of each extract in specific pathogen-free embryonated chicken eggs was estimated and their antiviral efficacy was determined in terms of reduction in viral titers, measured by the hemagglutination inhibition (HI) assay. The antiviral efficacy was compared with ribavirin. The active extract was further subjected to estimation of total polyphenolic and flavonoid contents. Also, GC/ MS analysis was used to analyze its unsaponifiable and saponifiable matters. Results: The cytotoxic concentration fifty (CC 50 ) of Breynia distacha recorded the value of >200 and >300 for Newcastle disease virus (NDV) and avian influenza virus (AIV) H5N1, respectively. This extract showed antiviral inhibition concentration (IC 50 ) values of ≤ 2 and ≤ 3, respectively and antiviral therapeutic index of 100% compared with ribavirin as standard. The serum of the vaccinated chicks treated with B. distacha has recorded the highest HI titers (7.1 and 8.3 log2) at the day of 28 post vaccination with high protection percentage (100 and 90%) post NDV and AIV H5N1 challenge, respectively. Also, a high HI titer (6.9 log2) was recorded in serum of the vaccinated chicks treated with Aleurites moluccana, Jatropha curcas, Ricinus communis, Putranjiva roxburghii with protection percentage of 95-100% post-NDV challenge. GC/MS analysis allowed identification of 91.10% of the total unsaponifiable matter, (1-heptatriacotanol represented the major component, 24.61%) and 82.51% of the fatty acid methyl esters content (methyl palmitate represented the major component, 26.13 %). Conclusion: This study highlighted the potential of many Euphorbiaceae plants especially B. distacha, to be new candidate of plant-based prototypes that carry antiviral and immunogenic features against respiratory viral infections.has the potential of being more successful in its steps than screening of pure compounds isolated from the products. 8 The spurge family, Euphorbiaceae occupied a special place in the realm of plant families and is scientifically reported for its antiviral, antimicrobial, anticancer, cytotoxic, and antitumor activities. 9 In this respect, the present work aims to search for the use of plants of the Euphorbiaceae family to combat emerging infectious poultry diseases. Fifteen species belong to this family, are widely distributed and easily available throughout various geographical locations in Egypt, 10 were selected and subjected to evaluate their potential anti-viral activities against respiratory tract infection pathogens in specific pathogen-free (SPF) chicken embryos and evaluation of immune boosting properties of these substances in SPF chicks. These plant species are well known to have their traditional claims for cure and/or reduce symptoms of various disorders including infectious diseases of animal and human among folk cultures.

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Innovation in Newcastle Disease Virus Vectored Avian Influenza Vaccines

Cited by 33 publications

References 66 publications

A Recombinant La Sota Vaccine Strain Expressing Multiple Epitopes of Infectious Bronchitis Virus (IBV) Protects Specific Pathogen-Free (SPF) Chickens against IBV and NDV Challenges

A Recombinant La Sota Vaccine Strain Expressing Multiple Epitopes of Infectious Bronchitis Virus (IBV) Protects Specific Pathogen-Free (SPF) Chickens against IBV and NDV Challenges

A recombinant avian paramyxovirus serotype 3 expressing the hemagglutinin protein protects chickens against H5N1 highly pathogenic avian influenza virus challenge

Evaluation of Immune Boosting Properties and Combating of Multiple Respiratory Viral Infections by fifteen Euphorbiaceae Plant Extracts

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