“…Kv2.1 immunohistochemistry was performed using mouse anti-Kv2.1 clone D4/11 at 1:1000 dilution that was developed and/or obtained from the UC Davis/NINDS/NIMH Neuromab facility, supported by NIH grant U24NS0506060 and maintained by the Department of Pharmacology, School of Medicine, University of California (Davis, CA, USA). All primary antibodies were diluted in PBS-T 0.1%, pH 7.4, and incubated overnight at 4 • C. The sensitivity and specificity of the primary antibodies against the various synaptic proteins and neuronal markers have been previously described by researchers in our laboratory (Alvarez et al 1997(Alvarez et al , 1999Deng & Fyffe, 2004;Muennich & Fyffe, 2004). Immunoreactivity was detected with species-specific secondary antibodies conjugated to fluorescein isothiocyanate, Cy3 or DyLight 649 (Jackson J Physiol 591.4 Immunoresearch, West Grove, PA, USA) diluted 1:50 in PBS-T 0.1%, pH 7.4, and incubated at room temperature for 2-4 h. Given that the antibodies against SK3 and SK2 were raised in the same host species (rabbit), the coexpression studies employed a sequential immunostaining process.…”