Innovative 3D Collagen Microsphere Scaffold (MSS) Promotes Robust Cellular Invasion

Asanbe, Ope; Hooper, Rachel C.; Elshazly, Tarek; Osoria, Hector L.; Jacoby, Adam; Joyce, Jeremiah; Weinreb, Ross H.; Stroock, Abraham D.; Spector, Jason A.; Walters, Ryan; Bernstein, Jaime L.; Vishnevsky, Bella; Morgan, John P.

doi:10.1097/01.prs.0000455350.91620.d3

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“…It is noteworthy that the protocol we optimized for the 3D scaffold production can be modified to obtain scaffolds of the desired shape and size, depending on the potential application (different from the CBSS). Indeed, the 3D scaffolds only (without 2D membranes) could be potentially employed for nerve regeneration in tubular conformation, as suggested by [51], for guided bone regeneration, if implemented with hydroxyapatite, as described by [52], to study if cell contraction and migration are influenced by scaffold conformation [50,51], to evaluate cell-ECM interactions [53], growth factors action on cell proliferation or silver nanoparticle antibiotic effects [54].…”

Section: Discussionmentioning

confidence: 99%

From Food Waste to Innovative Biomaterial: Sea Urchin-Derived Collagen for Applications in Skin Regenerative Medicine

et al. 2020

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Collagen-based skin-like scaffolds (CBSS) are promising alternatives to skin grafts to repair wounds and injuries. In this work, we propose that the common marine invertebrate sea urchin represents a promising and eco-friendly source of native collagen to develop innovative CBSS for skin injury treatment. Sea urchin food waste after gonad removal was here used to extract fibrillar glycosaminoglycan (GAG)-rich collagen to produce bilayer (2D + 3D) CBSS. Microstructure, mechanical stability, permeability to water and proteins, ability to exclude bacteria and act as scaffolding for fibroblasts were evaluated. Our data show that the thin and dense 2D collagen membrane strongly reduces water evaporation (less than 5% of water passes through the membrane after 7 days) and protein diffusion (less than 2% of BSA passes after 7 days), and acts as a barrier against bacterial infiltration (more than 99% of the different tested bacterial species is retained by the 2D collagen membrane up to 48 h), thus functionally mimicking the epidermal layer. The thick sponge-like 3D collagen scaffold, structurally and functionally resembling the dermal layer, is mechanically stable in wet conditions, biocompatible in vitro (seeded fibroblasts are viable and proliferate), and efficiently acts as a scaffold for fibroblast infiltration. Thus, thanks to their chemical and biological properties, CBSS derived from sea urchins might represent a promising, eco-friendly, and economically sustainable biomaterial for tissue regenerative medicine.

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