Innovative Electrochemical Approach for an Early Detection of microRNAs

Lusi, E. A.; Passamano, Myriam; Guarascio, P.; Scarpa, Aldo; Schiavo, Luigi

doi:10.1021/ac8026788

Cited by 123 publications

(75 citation statements)

References 13 publications

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“…New label-free strategies have been sought not only for recognition of specific DNA sequences or DNA damage, but also for detection and analysis of a new class of short RNA molecules, the so-called microRNAs, important in gene regulation. Oxidation peak of guanine at carbon electrode was recently used for label-free detection of microRNA in cancer [57]. Our results give promise that application of mercury electrodes such as SAE [50][51][52] can represent an alternative to carbon electrodes.…”

Section: Discussionmentioning

confidence: 73%

Square Wave Stripping Voltammetry of Unlabeled Single‐ and Double‐Stranded DNAs

Bartošík

Paleček

2011

Electroanalysis

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We show that, in difference to previously applied electrochemical methods working with stationary electrodes, square wave voltammetry produces well-developed peaks II SW (specific for dsDNA) and III SW yielded by ssDNA at hanging mercury drop electrode (HMDE) and solid amalgam electrodes (SAEs). Using these peaks various kinds of DNA structural transitions can be studied, including unwinding of dsDNA at negatively charged electrode surfaces. The sensitivity of the DNA analysis is much better than that obtained with guanine oxidation signals at carbon electrodes. Both carbon electrodes and SAEs appear attractive as transducers in label-free RNA and DNA sensors.

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Section: Discussionmentioning

confidence: 73%

Square Wave Stripping Voltammetry of Unlabeled Single‐ and Double‐Stranded DNAs

Bartošík

Paleček

2011

Electroanalysis

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“…Finally, methods for detecting miRNAs were diverse, among which RT-PCR was one of the most widely used approaches. Nevertheless many new methodologies emerged, such as the next-generation sequencing approach [101] and the electrochemical approach [102]. In short, a proper method for clinical application should be less expensive, reproducible, stable and with high sensitivity and specificity.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Value of miR-21 in Various Cancers: An Updating Meta-Analysis

et al. 2014

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BackgroundRecently, more and more studies investigated the value of microRNA (miRNA) as a diagnostic or prognostic biomarker in various cancers. MiR-21 was found dysregulated in almost all types of cancers. While the prognostic role of miR-21 in many cancers has been studied, the results were not consistent.MethodsWe performed a meta-analysis to investigate the correlation between miR-21 and survival of general cancers by calculating pooled hazard ratios (HR) and 95% confidence intervals (CI).ResultsThe pooled results of 63 published studies showed that elevated miR-21 was a predictor for poor survival of general carcinomas, with pooled HR of 1.91 (95%CI: 1.66–2.19) for OS, 1.42 (95% CI: 1.16–1.74) for DFS and 2.2 (95% CI: 1.64–2.96) for RFS/CSS. MiR-21 was also a prognostic biomarker in the patients who received adjuvant therapy, with pooled HR of 2.4 (95%CI: 1.18–4.9) for OS.ConclusionsOur results showed that miR-21 could act as a significant biomarker in the prognosis of various cancers. Further studies are warranted before the application of the useful biomarker in the clinical.

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“…In 2009, Lusi and his colleagues reported a miRNA biosensor based on the guanine oxidation after hybridization between the inosine-modified capture probes and the target miRNA (Lusi et al, 2009). The developed biosensor allowed a linear quantification of miRNA with a detection limit of 0.1 pM.…”

Section: Amperometric and Voltammetric Biosensorsmentioning

confidence: 99%