Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis

Abstract

BACKGROUND In this study, we aimed to evaluate the influence of different extraction procedures [decoction, homogenizer‐assisted extraction (HAE), infusion, maceration, Soxhlet and ultrasound‐assisted extraction (UAE)] on the chemical profiling and biological properties of methanol and water extracts of Pulicaria dysenterica (L.) Bernh. The chemical profiles of the extracts were evaluated by high‐performance liquid chromatography coupled to electrospray ionization and time‐of‐flight mass spectrometry (HPLC‐ESI‐TOF‐MS). The antioxidant properties and enzymes (lipase, α‐amylase, α‐glucosidase, tyrosinase and cholinesterases) inhibitory potential of the extracts were evaluated. RESULTS The chemical profiles were dependent on the type of extraction methods as well as on the type of solvent. The methanolic extracts showed higher levels of total phenolic, flavonoid, and phenolic acid content, while the highest total flavonol content was observed in the HAE–water extract. Forty different compounds were identified from P. dysenterica. In relation to the potential in vitro anti‐diabetic effects, the highest activity against the studied key enzymes was observed for the macerated extract (α‐amylase: 0.58 ± 0.03 and α‐ glucosidase: 1.65 ± 0.03 mmol ACAE g−1). The HAE–methanol extract was the most potent inhibitor of cholisterases, whereas the highest activities against tyrosinase were observed for UAE–methanol extract, followed by macerated and Soxhlet. The inhibitory activity of the studied extracts against lipase were in the order: soxhlet > macerated> HAE–methanol > UAE–methanol. CONCLUSION This study has established scientific baseline data on the therapeutic properties of P. dysentrica, thereby advocating the need for further investigations in an endeavour to develop novel pharmaceuticals from this plant. © 2019 Society of Chemical Industry

Two Medicinal Plants (Alkanna trichophila and Convolvulus galaticus) from Turkey: Chemical Characterization and Biological Perspectives

“…It is observed that apart from extraction methods, the types of solvents equally influenced the chemical composition of the extracts, as only solvents of the same polarity with the solutes (phytochemicals) will eventually dissolve the solutes. Such outcomes are in accordance with several other studies stating HAE using methanol as an efficient extraction technique in extracting high yield of biogenic substances [19–23] …”

The Influence of Different Extraction Techniques on the Chemical Profile and Biological Properties of Oroxylum indicum: Multifunctional Aspects for Potential Pharmaceutical Applications

“…Traditionally, ME and SOXE are widely applied to extract plant components from natural products [ 37 ]. However, they are time-consuming, labor-intensive, require large amounts of extraction solvents and have low extraction yield [ 38 , 39 ]. Therefore, in this case, nonconventional extraction techniques can be applied to extract phytochemical components from natural products [ 40 ].…”

Impact of Water Level on Species Quantity and Composition Grown from the Soil Seed Bank of the Inland Salt Marsh: An Ex-Situ Experiment

“…• Pulicaria dysenterica is to be found usually on wet and mildly saline soils [57] and is known for chemicals contained in its oils [58,59]. In the Czech Republic, it is scattered only across the valleys in southern Moravia and can be rarely seen in the White Carpathians, in Haná region, or the Moravian Gate and Ostrava regions [60].…”