Mulch is widely used in blueberry cultivation for weed control; however, there is still uncertainty as to how the use of different types of mulch alters leaf photosynthetic behavior and the quality and productivity of blueberry fruit. The objective of our research was to evaluate the effect of different types of mulch on the physiological, quality and yield characteristics of blueberries. Three treatments were established: T1 (control), T2 (pine bark) and T3 (geotextile) in two cultivars: Ochlockonee and Legacy. The parameters measured were: the photochemical quantum yield of photosystem II (YII), the maximum photochemical efficiency of photosystem II (Fv/Fm), electron transport rate (ETR), fruit quality and yield parameters. The results show lower soil temperature in T1 during the morning (p < 0.05) compared to the two mulch treatments, which was the opposite during the afternoon, the temperatures were more stable and closer to the optimum (21 °C) in T2 and T3, with mulch favoring root and foliar development. On the other hand, the treatments with mulch favored a higher photosynthetic efficiency of photosystem II (YII) at the end of afternoon and were associated with an increased firmness of the fruit; the firmness of all fruits was higher than that in the control treatment (p < 0.05) in the Legacy cultivar, but without differences between them, with values of 73 and 75 gf mm−1 for T2 and T3, respectively, and 67 gf mm−1 for the Control. In addition, it was observed that the use of mulch only increased the fruit yield in the Legacy cultivar, both in T2 and T3, with both being superior to T1 (p < 0.05). It can be concluded that the use of mulch decreases soil temperature in the midday and late afternoon, improving the edaphoclimatic conditions during the development of the blueberry. In addition, plants with mulch have lower stomatal conductance, which promotes greater photosynthetic efficiency during the day, increasing both firmness and fruit yield.