Innovative techniques for collection of saturated and unsaturated subsurface basalts and sediments for microbiological characterization

Colwell, F. S.; Stormberg, G.J; Phelps, Tommy J.; Birnbaum, S. J.; McKinley, J. P.; Rawson, S.A.; Veverka, C.; Goodwin, Shannon M.; Long, Philip E.; Russell, B.F; Garland, T.; Thompson, D.L.; Skinner, P; Grover, Samir C.

doi:10.1016/0167-7012(92)90047-8

Cited by 94 publications

(68 citation statements)

References 10 publications

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“…Subsurface samples were obtained as part of an cxpcriment to determine whether viable bacteria exist in geological strata that probably have been isolated hydrologically from the surface for more than 10' years. Samples were taken laterally from the sidewall of a well (10). The samples, which were obtained from 2.7 km below the land surface in the Taylorsville Triassic Basin in Virginia, consisted of fine-grained, laminated siltstone (2, 5 , 6).…”

Section: Methodsmentioning

confidence: 99%

“…The samples were inoculated into enrichment cultures for a variety of bacterial physiological groups, and predominantly anaerobic thermophilic bacteria, including fermentative, iron-reducing, sulfate-reducing, and denitrifying bacteria, grew (5,6). The possibility that the organisms might be the result of contamination during the drilling and sampling process was eliminated by several lines of evidence (2,5,10). Two of the most significant lines of evidence were the finding that some bacteria occurred in very large numbers in the drilling mud yet were not present in detectable numbers in the samples and the finding that when large numbers of microscopic latex beads were added to the drilling mud at the site of sample collection, beads were absent from the pared rock samples.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Description of Two New Thermophilic Desulfotomaculum spp., Desulfotomaculum putei sp. nov., from a Deep Terrestrial Subsurface, and Desulfotomaculum luciae sp. nov., from a Hot Spring

Liu

Karnauchow

Jarrell

et al. 1997

International Journal of Systematic and Evolutionary Microbiology

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Description of Two New Thermophilic Desulfotomaculum spp., Desulfotomaculum putei sp. nov., from a Deep Terrestrial Subsurface, and Desulfotomaculum luciae sp. nov., from a Hot Spring

Liu

Karnauchow

Jarrell

et al. 1997

International Journal of Systematic and Evolutionary Microbiology

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“…Microorganismsin the solid phase of hard rock aquifers can best be analysed in rock cores from drillings (Colwell et al 1992). As microbial contamination is likely to occur during the drilling process, laboratory analyses should be done in samples taken from the interior of the cores (Krumholz et al 1997).…”

Section: Sampling Techniquesmentioning

confidence: 99%

Review: Microbial biocenoses in pristine aquifers and an assessment of investigative methods

2006

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The current knowledge of microbial biocenoses (communities) in pristine aquifers is presented in a review, which also discusses their relevance for questions of groundwater protection. Aquifers are heterogeneous on all scales and structured in a variety of habitats. The void spaces in many aquifers are small. The biocenoses are thus predominantly composed of microorganisms and, often, microinvertebrates. Larger voids and macroorganisms occur in karst cavities. Due to the absence of light, the biocenoses depend on chemical energy resources, which are, however, scarce in non-contaminated groundwater. The microorganisms thus show small cell sizes, low population densities and reduced activity; they developed specific strategies to survive oligotrophic conditions. The review also discusses the impact of contamination on the biocenoses, and the potential use of the biocenoses or specific organisms as indicators for groundwater quality, and the limits of this approach. Bacteria are either planktonic or attached to aquifer material, which requires both fluid and solid phase sampling. Most groundwater bacteria are viable but non-culturable. Consequently, cultivation techniques give an incomplete picture of the biocenoses, while methods from molecular microbiology provide genetic fingerprints of the entire community. Different analytical methods are available to count microorganisms, identify species, characterise microbial diversity, and measure activity.Résumé Cette revue expose l'état actuel des connaissances concernant les biocénoses microbiennes présentes dans les aquifères oligotrophes. L'impact d'une contamination sur les biocénoses est discuté, ainsi que le potentiel que Les bactéries sont planctoniques ou attachées aux matériaux de l'aquifère, ce qui demande unéchantillonnagè a la fois de l'eau et du substrat. De nombreuses méthodes sont aujourd'hui disponibles pour le comptage, l'identification et la caractérisation de la diversité, ainsi que la mesure des activités des organismes des aquifères. Comme la grande majorité des bactéries est viable mais non cultivable, les techniques de cultures actuelles ne donnent qu'une image incomplète des communautés, alors que les méthodes moléculaires développées récemment offrent la possibilité d'obtenir un profil de la communauté plus complet.Resumen Se presentauna reseña crítica del conocimiento actual de biocenosis microbiana (comunidades) en acuíferos prístinos la cual también discute su relevancia en términos de protección de aguas subterráneas. Los acuíferos son heterogéneos en todas las escalas y estructurados en una variedad de habitats. Los espacios vacíos en muchos acuíferos son pequeños. La biocenosis está por lo tanto compuesta predominantemente por microorganismos y, frecuentemente, microinvertebrados. Espacios más grandes y macroorganismos ocurren en cavidades kársticas. Debido a la ausencia de luz la biocenosis depende de recursos energéticos químicos los cuales, sin embargo, son escasos en agua subterránea no contaminada. Los microorganismos muestran...

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“…We monitored the intrusion potential of drilling fluid during the coring operations by adding a water-soluble perfluorocarbon tracer (PFT) to the drilling fluid and subsequently measuring the PFT concentration in sediment samples immediately adjacent to dedicated microbiology samples. The addition of PFT to drilling fluid is an effective way to quantify drilling-induced contamination, as it is inert and can be detected with high sensitivity (Colwell et al, 1992). The measured PFT concentration in the sediment can then be used as an indirect measure of drilling fluid intrusion and potential associated entrainment of nonindigenous cells.…”

Section: Introductionmentioning

confidence: 99%

Data report: quantification of potential drilling contamination using perfluorocarbon tracer at IODP Expedition 329 sites

Sauvage¹,

Lewis²,

Graham³

et al. 2017

Proceedings of the IODP

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Innovative techniques for collection of saturated and unsaturated subsurface basalts and sediments for microbiological characterization

Cited by 94 publications

References 10 publications

Description of Two New Thermophilic Desulfotomaculum spp., Desulfotomaculum putei sp. nov., from a Deep Terrestrial Subsurface, and Desulfotomaculum luciae sp. nov., from a Hot Spring

Description of Two New Thermophilic Desulfotomaculum spp., Desulfotomaculum putei sp. nov., from a Deep Terrestrial Subsurface, and Desulfotomaculum luciae sp. nov., from a Hot Spring

Review: Microbial biocenoses in pristine aquifers and an assessment of investigative methods

Data report: quantification of potential drilling contamination using perfluorocarbon tracer at IODP Expedition 329 sites

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