Inoculation of coffee plants with the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales)

Posada, Francisco; Aime, M. Catherine; Peterson, Stephen W.; Rehner, Stephen A.; Vega, Fernando E.

doi:10.1016/j.mycres.2007.03.006

Cited by 231 publications

(206 citation statements)

References 48 publications

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“…Beauveria bassiana (Balsamo) Vullemin (Ascomycota: Hypocreales) is the best-studied endophytic fungal entomopathogen [5][6][7][8][9][11][12][13][14][15][16][17][18][19] , and it is available as a commercial mycopesticide. Inoculation methods tested to establish B. bassiana as an endophyte include soil drenches 14,17 , seed coatings 18 and immersions 14 , radicle dressings 13,15 , root and rhizome immersions 11,16,18 , .…”

Section: Introductionmentioning

confidence: 99%

“…Inoculation methods tested to establish B. bassiana as an endophyte include soil drenches 14,17 , seed coatings 18 and immersions 14 , radicle dressings 13,15 , root and rhizome immersions 11,16,18 , . Recent evidence suggests that endophytic B. bassiana has the potential to protect plants not only from arthropod pests [5][6][7][22][23][24][25][26][27] , but also from some plant pathogens 9 .…”

Section: Introductionmentioning

confidence: 99%

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Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Parsa

Ortiz

Vega

2013

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Beauveria bassiana is a fungal entomopathogen with the ability to colonize plants endophytically. As an endophyte, B. bassiana may play a role in protecting plants from herbivory and disease. This protocol demonstrates two inoculation methods to establish B. bassiana endophytically in the common bean (Phaseolus vulgaris), in preparation for subsequent evaluations of endophytic biological control. Plants are grown from surface-sterilized seeds for two weeks before receiving a B. bassiana treatment of 10 8 conidia/ml (or water) applied either as a foliar spray or a soil drench. Two weeks later, the plants are harvested and their leaves, stems and roots are sampled to evaluate endophytic fungal colonization. For this, samples are individually surface sterilized, cut into multiple sections, and incubated in potato dextrose agar media for 20 days. The media is inspected every 2-3 days to observe fungal growth associated with plant sections and record the occurrence of B. bassiana to estimate the extent of its endophytic colonization. Analyses of inoculation success compare the occurrence of B. bassiana within a given plant part (i.e. leaves, stems or roots) across treatments and controls. In addition to the inoculation method, the specific outcome of the experiment may depend on the target crop species or variety, the fungal entomopathogen species strain or isolate used, and the plant's growing conditions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Parsa

Ortiz

Vega

2013

JoVE

Self Cite

124

123

View full text Add to dashboard Cite

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“…It is important to determine the appropriate concentration of the fungal pathogen cell suspension culture used for inoculation [8][9][10][11]22 . Inoculation with high concentrations of the pathogen cell suspension culture will cause the death of both resistant and susceptible plants, while low concentrations will not show a phenotype on either plant type.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, development and use of an efficient inoculation method would allow many plants to be inoculated and screened for resistance. Various inoculation methods have been used including dip inoculation, pipetting the pathogen cell suspension culture into the whirl of the plant, and needle injection inoculation [8][9][10][11] . With each method, the pathogen must reliably be introduced in between the plant leaves where the pathogen enters the plant through the formation of appresoria to ensure pathogen development and plant infection 12,13 .…”

Section: Introductionmentioning

confidence: 99%

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A Rapid and Efficient Method for Assessing Pathogenicity of <em>Ustilago maydis</em> on Maize and Teosinte Lines

Chavan

Smith

2014

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Maize is a major cereal crop worldwide. However, susceptibility to biotrophic pathogens is the primary constraint to increasing productivity. U. maydis is a biotrophic fungal pathogen and the causal agent of corn smut on maize. This disease is responsible for significant yield losses of approximately $1.0 billion annually in the U.S.1 Several methods including crop rotation, fungicide application and seed treatments are currently used to control corn smut 2 . However, host resistance is the only practical method for managing corn smut. Identification of crop plants including maize, wheat, and rice that are resistant to various biotrophic pathogens has significantly decreased yield losses annually [3][4][5] . Therefore, the use of a pathogen inoculation method that efficiently and reproducibly delivers the pathogen in between the plant leaves, would facilitate the rapid identification of maize lines that are resistant to U. maydis. As, a first step toward indentifying maize lines that are resistant to U. maydis, a needle injection inoculation method and a resistance reaction screening method was utilized to inoculate maize, teosinte, and maize x teosinte introgression lines with a U. maydis strain and to select resistant plants.Maize, teosinte and maize x teosinte introgression lines, consisting of about 700 plants, were planted, inoculated with a strain of U. maydis, and screened for resistance. The inoculation and screening methods successfully identified three teosinte lines resistant to U. maydis. Here a detailed needle injection inoculation and resistance reaction screening protocol for maize, teosinte, and maize x teosinte introgression lines is presented. This study demonstrates that needle injection inoculation is an invaluable tool in agriculture that can efficiently deliver U. maydis in between the plant leaves and has provided plant lines that are resistant to U. maydis that can now be combined and tested in breeding programs for improved disease resistance. Video LinkThe video component of this article can be found at

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Entophytic Microbes and Biocontrol of Plant Diseases

Srivastava

Tripathi

Pandey³

2014

Biological Controls for Preventing Food Deterioration

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Inoculation of coffee plants with the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales)

Cited by 231 publications

References 48 publications

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control

A Rapid and Efficient Method for Assessing Pathogenicity of <em>Ustilago maydis</em> on Maize and Teosinte Lines

Entophytic Microbes and Biocontrol of Plant Diseases

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