Inoculation of Rhizoglomus irregulare or Trichoderma atroviride differentially modulates metabolite profiling of wheat root exudates

Lucini, Luigi; Colla, G.; Miras-Moreno, Begoña; Bernardo, Letizia; Cardarelli, Mariateresa; Terzi, Valeria; Bonini, Paolo; Rouphael, Youssef

doi:10.1016/j.phytochem.2018.10.033

Cited by 83 publications

(51 citation statements)

References 49 publications

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“…Root exudates were filtered through a 0.22 um cellulose syringe filter and metabolic profiling was carried out by UHPLC-ESI/QTOF mass spectrometry as described by Lucini et al 69 . Chromatographic separation was achieved using water and CH 3 CN as the mobile phases (both LCMS grade, VWR, Milan, Italy).…”

Section: Methodsmentioning

confidence: 99%

“…Raw spectral data were processed using the Profinder B.06 software (Agilent Technologies) and compared to an in-house database specific for plant root exudates 20 , 69 . The find-by-formula algorithm was used to annotate molecular features, following mass and retention time alignment, using monoisotopic accurate mass, isotope spacing and isotope ratio.…”

Section: Methodsmentioning

confidence: 99%

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Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

Marastoni

Lucini

Miras-Moreno

et al. 2020

Sci Rep

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In several cultivation areas, grapevine can suffer from Fe chlorosis due to the calcareous and alkaline nature of soils. This plant species has been described to cope with Fe deficiency by activating Strategy I mechanisms, hence increasing root H+ extrusion and ferric-chelate reductase activity. The degree of tolerance exhibited by the rootstocks has been reported to depend on both reactions, but to date, little emphasis has been given to the role played by root exudate extrusion. We studied the behaviour of two hydroponically-grown, tolerant grapevine rootstocks (Ramsey and 140R) in response to Fe deficiency. Under these experimental conditions, the two varieties displayed differences in their ability to modulate morpho-physiological parameters, root acidification and ferric chelate reductase activity. The metabolic profiling of root exudates revealed common strategies for Fe acquisition, including ones targeted at reducing microbial competition for this micronutrient by limiting the exudation of amino acids and sugars and increasing instead that of Fe(III)-reducing compounds. Other modifications in exudate composition hint that the two rootstocks cope with Fe shortage via specific adjustments of their exudation patterns. Furthermore, the presence of 3-hydroxymugenic acid in these compounds suggests that the responses of grapevine to Fe availability are rather diverse and much more complex than those usually described for Strategy I plants.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

Marastoni

Lucini

Miras-Moreno

et al. 2020

Sci Rep

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“…Thereafter, the raw data were re-processed against an in-house database, manually curated to include the intermediate biosynthetic compounds and the end products for gramine, hordenine and N-methyltyramine. Elaboration of metabolomics dare were formerly carried out using Agilent Mass Profiler Professional B.12.06 (from Agilent Technologies) as described by [48]. Compounds were filtered by abundance (area >10000 counts), normalization at the 75th percentile and baselined to the median of the control.…”

Section: Untargeted Metabolomicsmentioning

confidence: 99%

New insights in the allelopathic traits of different barley genotypes: Middle Eastern and Tibetan wild-relative accessions vs. cultivated modern barley

et al. 2020

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The two alkaloids gramine and hordenine have been known for playing a role in the allelopathic ability in barley (Hordeum vulgare L.). These allelochemicals can be both found in leaves and roots in some barley cultivars whereas in others one seems to exclude the other. In this study eighteen accessions of barley from the Middle-East area, one accession from Tibet and the modern spring cultivar Barke, already used as parental donor in a nested associated mapping (NAM) population, were screened for their gramine, hordenine and Nmethyltyramine (the direct precursor of hordenine) content in leaves, roots and exudates. Moreover, the toxicity of the three allelochemicals on root growth inhibition on lettuce (Lactuca sativa L.) was evaluated. Results of this study showed the preferential production of gramine and hordenine in leaves and roots, respectively, in the nineteen barley accessions. On the other hand, in the modern barley cultivar Barke, the highest content of hordenine in roots and the general lack of gramine suggests a favored biosynthesis of the former. Gramine was not detected in the root exudates. In additions, different metabolomic profiles were observed in wild relatives compared to modern barley genotypes. The results also showed the phytotoxic effects of the three compounds on root growth of lettuce seedlings, with a reduction in root length and an increase of root surface area and diameter. In conclusion, this study highlighted the impact of the domestication effects on the production and distribution of the two allelopathic alkaloids gramine and hordenine in barley.

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“…Inoculation with T. virens GV41 increased N uptake of both lettuce and rocket in non-fertilized plots, with values not different than N1-T0, even if values were higher than N2-T1 treatment. The marked effect of Trichoderma inoculation under abiotic stress conditions such as nutrient deficiency and low water availability has also been recognized by other authors for vegetable crops [50].…”

Section: Discussionmentioning

confidence: 52%

Can Trichoderma-Based Biostimulants Optimize N Use Efficiency and Stimulate Growth of Leafy Vegetables in Greenhouse Intensive Cropping Systems?

et al. 2020

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The present study addresses the effects of Trichoderma-based biostimulants and nitrogen (N) fertilization levels on agronomic performance and functional quality of two important greenhouse leafy vegetables: lettuce and rocket. A factorial analysis of the relative effects of Trichoderma-based biostimulants (Trichoderma harzianum strain T22 and Trichoderma virens strain GV41) and N fertilization levels (sub-optimal, optimal, and supra-optimal) was carried out to evaluate crop productive behavior (marketable and unmarketable yields, leaf dry matter content, and biomass production), nitrogen nutrition (N uptake, apparent N recovery, and nitrogen use efficiency (NUE)) as well as phytochemical qualitative components (antioxidant activity and total polyphenols). The soil plant analysis development (SPAD) index in both leafy vegetables and leaf colorimetry only in rocket were mainly affected by N fertilization levels but not by Trichoderma-based biostimulants. The contribution of native mineral N was 60 and 100 kg N ha−1 of the total uptake in lettuce and rocket, respectively, and N surpluses were observed in both crops, even under optimal fertilization conditions. Trichoderma virens GV41-based biostimulant increased lettuce marketable yield and biomass production, both under optimal and sub-optimal fertilization. In addition, the same treatment increased NUE up to 116% under recommended N fertilization, that was also associated to an increase in phenol content and antioxidant activity. Rocket showed a clear effect of the Trichoderma virens GV41 treatment, only in absence of fertilization, demonstrating an increase in marketable yield and N uptake. Thus, the inoculation of rocket with this Trichoderma biostimulant can be considered as a useful management tool in leafy vegetable cropping systems for the efficient use of residual fertilizers from previous crops, enhancing NUE within the crop rotations. Nevertheless, the application of microbial biostimulant treatments requires good monitoring of soil N fertility in order to avoid an overexploitation of soil N supplying potential.

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Inoculation of Rhizoglomus irregulare or Trichoderma atroviride differentially modulates metabolite profiling of wheat root exudates

Cited by 83 publications

References 49 publications

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

Changes in physiological activities and root exudation profile of two grapevine rootstocks reveal common and specific strategies for Fe acquisition

New insights in the allelopathic traits of different barley genotypes: Middle Eastern and Tibetan wild-relative accessions vs. cultivated modern barley

Can Trichoderma-Based Biostimulants Optimize N Use Efficiency and Stimulate Growth of Leafy Vegetables in Greenhouse Intensive Cropping Systems?

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