Inoculation with a Pb-tolerant strain of Paxillus involutus improves growth and Pb tolerance of Populus × canescens under in vitro conditions

Szuba, Agnieszka; Karliński, Leszek; Krzesłowska, Magdalena; Hazubska-Przybył, Teresa

doi:10.1007/s11104-016-3062-3

Cited by 43 publications

(47 citation statements)

References 60 publications

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“…A similar effect of Pb was observed on Pisum sativum by Rodriguez et al [57] and on corn by Nyitrai et al [58]. An increase in the chlorophyll content that was caused by Pb was also confirmed in Populus × canescenc trees [59] and Arabis paniculata [45]. The results presented in the current study also confirmed that the phenomenon of hormesis, which is caused by Pb, was positively correlated with a higher chlorophyll content and that there was no significant difference in the PSII yield compared to the control (Figures 3b, 5 and 6b).…”

Section: Discussionsupporting

confidence: 76%

Hormesis in Plants: The Role of Oxidative Stress, Auxins and Photosynthesis in Corn Treated with Cd or Pb

Małkowski

Sitko

Szopiński

et al. 2020

IJMS

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Hormesis, which describes the stimulatory effect of low doses of toxic substances on growth, is a well-known phenomenon in the plant and animal kingdoms. However, the mechanisms that are involved in this phenomenon are still poorly understood. We performed preliminary studies on corn coleoptile sections, which showed a positive correlation between the stimulation of growth by Cd or Pb and an increase in the auxin and H 2 O 2 content in the coleoptile sections. Subsequently, we grew corn seedlings in hydroponic culture and tested a wide range of Cd or Pb concentrations in order to determine hormetic growth stimulation. In these seedlings the gas exchange and the chlorophyll a fluorescence, as well as the content of chlorophyll, flavonol, auxin and hydrogen peroxide, were measured. We found that during the hormetic stimulation of growth, the response of the photosynthetic apparatus to Cd and Pb differed significantly. While the application of Cd mostly caused a decrease in various photosynthetic parameters, the application of Pb stimulated some of them. Nevertheless, we discovered that the common features of the hormetic stimulation of shoot growth by heavy metals are an increase in the auxin and flavonol content and the maintenance of hydrogen peroxide at the same level as the control plants.

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Section: Discussionsupporting

confidence: 76%

Hormesis in Plants: The Role of Oxidative Stress, Auxins and Photosynthesis in Corn Treated with Cd or Pb

Małkowski

Sitko

Szopiński

et al. 2020

IJMS

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“…Populus × canescens microcuttings (clones) provided by Prof. Krystyna Bojarczuk of the Institute of Dendrology, Polish Academy of Sciences, were propagated in a modified Murashige and Skoog (MS) agar medium containing 1.5% sucrose, as described previously (Szuba et al 2017). Paxillus involutus isolates were obtained from fruit bodies growing in poplar monocultures and maintained in vitro on agar in a modified Melin-Norkrans medium (MMN; Kottke et al 1987) containing 1% glucose and 0.5% maltose.…”

Section: In Vitro Cultures and Experimental Designmentioning

confidence: 99%

“…ECM symbiosis can lead to altered leaf anatomies or larger leaf sizes (Martins et al 1997) that could increase a plant's photosynthetic abilities. ECM fungi can influence, for example, the N status of leaves, which results in an increase (Reid et al 1983;Hobbie and Colpaert 2003) or decrease (Hobbie and Colpaert 2003;Koele et al 2012;Näsholm et al 2013;Szuba et al 2017) in foliar N concentrations. Thus, these changes may be consequences of leaf proteome modifications by ECM symbiosis.…”

Section: Introductionmentioning

confidence: 99%

“…Although N uptake can occur via a mantle (Garcia et al 2016), it is commonly believed that the resource exchange mainly occurs via the Hartig net-a network of fungal cells located between epidermal and cortical plant cells (Smith and Read 2008). As the formation of the ectomycorrhizae proceeds, the architecture of root tips and thus their functioning changes, e.g., due to altered nutrient uptake in root tips, devoid of root hairs but still without well-developed Hartig nets (Smith and Read 2008;Szuba et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of the leaf proteome by inoculation of Populus × canescens with two Paxillus involutus isolates differing in root colonization rates

et al. 2019

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During ectomycorrhizal symbioses, up to 30% of the carbon produced in leaves may be translocated to the fungal partner. Given that the leaf response to root colonization is largely unknown, we performed a leaf proteome analysis of Populus × canescens inoculated in vitro with two isolates of Paxillus involutus significantly differing in root colonization rates (65 ± 7% vs 14 ± 7%), together with plant growth and leaf biochemistry analyses to determine the response of plant leaves to ectomycorrhizal root colonization. The isolate that more efficiently colonized roots (isolate H) affected 9.1% of the leaf proteome compared with control plants. Simultaneously, ectomycorrhiza in isolate H-inoculated plants led to improved plant growth and an increased abundance of leaf proteins involved in protein turnover, stress response, carbohydrate metabolism, and photosynthesis. The protein increment was also correlated with increases in chlorophyll, foliar carbon, and carbohydrate contents. Although inoculation of P. × canescens roots with the other P. involutus isolate (isolate L, characterized by a low root colonization ratio) affected 6.8% of the leaf proteome compared with control plants, most proteins were downregulated. The proteomic signals of increased carbohydrate biosynthesis were not detected, and carbohydrate, carbon, and leaf pigment levels and plant biomass did not differ from the noninoculated plants. Our results revealed that the upregulation of the photosynthetic protein abundance and levels of leaf carbohydrate are positively related to rates of root colonization. Upregulation of photosynthetic proteins, chlorophyll, and leaf carbohydrate levels in ectomycorrhizal plants was positively related to root colonization rates and resulted in increased carbon translocation and sequestration underground. Keywords Ectomycorrhiza. Root colonization rate. Plant biometrics. Protein turnover. Stress response. Leaf carbohydrates Abbreviations 2DE Two-dimensional gel electrophoresis Chl a Chlorophyll a Chl b Chlorophyll b DW Dry weight FW Fresh weight ECM Ectomycorrhizal Rca RuBisCo activase SC Soluble carbohydrates SEM Scanning electron microscopy TNC Total non-structural carbohydrates Electronic supplementary material The online version of this article (

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“…Choi et al [29] and Makita et al [30] have suggested that C allocated to the belowground parts under P limitation is for hyphal growth, fungal respiration, and other fungal metabolic demands, which could greatly increase the nutrient capturing capability of the plant [31]. Although~20% of the carbon assimilated by the plant was consumed by the fungal symbiont [32], ECM fungi associations might prevent the formation of root hairs and thus inhibit root growth [33][34][35]. This might result in relatively lower C in the belowground parts, and thus more C was allocated aboveground [36].…”

Section: Aluminum Resistance and Biomass Production In Ecm P Massonimentioning

confidence: 99%

Accumulation and Translocation of Phosphorus, Calcium, Magnesium, and Aluminum in Pinus massoniana Lamb. Seedlings Inoculated with Laccaria bicolor Growing in an Acidic Yellow Soil

Wang

et al. 2019

Forests

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Research Highlights: We demonstrate that ectomycorrhizal (ECM) fungi improve plant aluminum (Al)-tolerance in the field and Laccaria bicolor S238A is a promising ECM isolate. Furthermore, we interpret the underlying nutritional mechanism that ECM inoculation facilitates aboveground biomass production as well as nutrients accumulation and translocation. Background and Objectives: Al toxicity is a primary limiting factor for plants growing in acidic soils. Hydroponic/sand culture studies have shown that some ECM fungi could enhance plant Al-tolerance. However, the underlying mechanisms of ECM fungi in improving plant Al-tolerance in the field are still unknown. To fill this knowledge gap, the present study aimed to examine roles of ECM inoculation in biomass production, accumulation and translocation of nutrients and Al in the host plant grown in the field under Al treatment. Materials and Methods: 4-week-old Pinus massoniana seedlings were inoculated with three Laccaria bicolor isolates (L. bicolor 270, L. bicolor S238A or L. bicolor S238N) and grown in an acidic yellow soil under 1.0 mM Al treatment for 12 weeks in the field. Biomass production, accumulation and translocation of P, Ca, Mg, and Al were investigated in these 16-week-old P. massoniana seedlings. Results: All three of these L. bicolor isolates improved biomass production as well as P, Ca and Mg accumulation in P. massoniana seedlings. Moreover, the three ECM isolates facilitated the translocation of P, Ca, and Mg to aboveground in response to Al treatment, particularly when seedlings were inoculated with L. bicolor S238A. In addition, both L. bicolor 270 and L. bicolor S238A had no apparent effects on Al accumulation, while enhanced Al translocation to aboveground. In contrast, L. bicolor S238N decreased Al accumulation but had no significant effect on Al translocation. Conclusions: ECM fungi in the field improved plant Al-resistance by increasing nutrient uptake, and this was mostly due to translocation of P, Ca, and Mg to aboveground, not by decreasing the uptake and translocation of Al.

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Inoculation with a Pb-tolerant strain of Paxillus involutus improves growth and Pb tolerance of Populus × canescens under in vitro conditions

Cited by 43 publications

References 60 publications

Hormesis in Plants: The Role of Oxidative Stress, Auxins and Photosynthesis in Corn Treated with Cd or Pb

Hormesis in Plants: The Role of Oxidative Stress, Auxins and Photosynthesis in Corn Treated with Cd or Pb

Regulation of the leaf proteome by inoculation of Populus × canescens with two Paxillus involutus isolates differing in root colonization rates

Accumulation and Translocation of Phosphorus, Calcium, Magnesium, and Aluminum in Pinus massoniana Lamb. Seedlings Inoculated with Laccaria bicolor Growing in an Acidic Yellow Soil

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