Inoculation with Penicillium citrinum aids ginseng in resisting Fusarium oxysporum by regulating the root and rhizosphere microbial communities

Wang, Liwei; Zhang, Yabo; Wang, Yan; Suo, Meng; Wu, Hao; Zhao, Min; Hong-yan, Yang

doi:10.1016/j.rhisph.2022.100535

Cited by 10 publications

(4 citation statements)

References 63 publications

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“…The six fungal isolates in our study were identified as F. oxysporum , A. alternata , P. grisea and C. truncatum , species of pathogenic fungi that can infect many different crops (Nguvo & Gao, 2019). F. oxysporum is largely responsible for causing root rot in Sanqi (Fan et al, 2016) as well as in ginseng (Wang, Zhang, Wang, et al, 2022) and soybean (Cruz Jimenez et al, 2018). A. panax has been found to be the main cause of Alternaria leaf spots in ginseng plants (Li et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Improper crop rotation may enrich soil‐borne pathogens of Panax notoginseng

Yang

Wei

et al. 2023

Journal of Phytopathology

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Soil‐borne diseases are the main cause of yield reduction of Panax notoginseng (Sanqi), and are mainly caused by the enrichment of pathogenic fungi during continuous cropping. In the Wenshan district of Yunnan province, China, where Sanqi is widely cultivated, the rotation of Foeniculum vulgare (fennel) crops with Sanqi crops is assumed to help reduce occurrences of rot in Sanqi roots. However, in a field investigation, we found that this practice actually increased incidences of root rot in Sanqi crops. Using fennel plants obtained from the cropping system, we tested the hypothesis that fennel crops enriched communities of pathogenic fungi of Sanqi. We isolated six endophytic fungi from the roots of fennel plants and identified these based on their morphological characteristics and a sequencing analysis. The isolates were identified as Fusarium oxysporum (FV‐1‐R‐1, FV‐2‐R‐1), Alternaria alternata (FV‐3‐R‐1, FV‐14‐R‐1), Pyricularia grisea (FV‐8‐R‐1) and Colletotrichum truncatum (FV‐11‐R‐4). In a series of inoculation experiments, we verified the pathogenicity of these fungi to Sanqi based on Koch's postulates, and demonstrated that all isolates caused root rot in Sanqi. Our results suggest that fennel is an inappropriate crop choice for rotation with Sanqi because it may serve as an intermediate host for the pathogenic fungi that cause root rot in Sanqi, and thereby exacerbate crop diseases. Our empirical findings provide useful information for enhancing the cultivation of Sanqi and the practice of crop rotation.

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Section: Discussionmentioning

confidence: 99%

Improper crop rotation may enrich soil‐borne pathogens of Panax notoginseng

Yang

Wei

et al. 2023

Journal of Phytopathology

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“…Some Penicillium species are involved in the production of solubilized phosphorus, siderophore, and phytohormones such as indole acetic acid and gibberellic acid, all of which are beneficial to plant health ( Elias et al, 2016 ; Altaf et al, 2018 ). Penicillium species have shown to suppress the root rot disease caused by Fusarium species and promote plant growth ( Wang et al, 2022a ). Fusarium species like F. oxysporum and F. solani are the major cause of tobacco root rot disease ( Yang et al, 2020a ; Gai et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Differentially enriched fungal communities in root rot resistant and susceptible varieties of tobacco (Nicotiana tabacum L.) under continuous monoculture cropping

Wang²,

Yang³

et al. 2022

Front. Microbiol.

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Root rot is a major disease of tobacco that causes crop losses of up to 15–20% of global tobacco production. The present study aimed to compare the fungal communities, and physicochemical properties of rhizosphere soil of root rot resistant (Yunyan 87; Y) and susceptible (Honghua Dajinyuan; H) tobacco varieties. Four treatments of each variety under continuous monocropping cultures included: control groups (HT0 and YT0); 2 years of continuous cropping (HT2 and YT2); 4 years of continuous cropping (HT4 and YT4); and 8 years of continuous cropping (YT8 and HT8). The soil physicochemical properties including available nitrogen (AN), available phosphorus (AP), available potassium (AK), and organic matter (OM) were increased (p < 0.05) from HT0 to HT8, whereas the resistant variety (Y) showed an inconsistent trend from YT0 to YT8. The pH was decreased (p < 0.05) from HT0 to HT8 and YT0 to YT8. Further, the disease incidence rate and disease index of the H variety also increased (p < 0.05) from HT0 to HT8. Alpha diversity analysis revealed that susceptible variety had higher fungal diversity from HT0 to HT8, while resistant variety exhibited lower diversity from YT0 to YT8. Ascomycota and Mortierellomycota were the dominant phyla in H and Y. Ascomycota abundance was increased (p < 0.05), whereas Mortierellomycota was decreased (p < 0.05) for continuous cropping years in H and Y. Penicillium, Fusarium, and Chrysosporium were the top three abundant genera in both varieties. The relative abundance of Penicillium spp. was increased (p < 0.05) in Y, whereas decreased (p < 0.05) in H variety. Specifically, Chrysosporium spp. was increased (p < 0.05) whereas Fusarium spp. was decreased (p < 0.05) in YT2. Redundancy analysis (RDA) revealed that fungal communities in H and Y rhizospheres were influenced by pH and carbon content, respectively. The top three highly enriched (p < 0.05) pathways in both varieties were fatty acid elongation, fatty acid β-oxidation I, and glyoxylate cycle. Our study concluded that resistant variety exhibited lower fungal diversity and functionally enriched metabolic pathways than susceptible variety that might be the result of molecular breeding practices, however, the relative abundance of Penicillium spp. were increased in resistant variety under long-term monoculture cropping.

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“…Bacterial PCRs were performed in triplicate with 4 µL of 5 × FastPfu Buffer, 2 µL of 2.5 mmol/L dNTPs, 0.8 µL of 5 µmol/L forwards primer, 0.8 µL of 5 µmol/L reverse primer, 0.4 µL of Fastpfu Polymerase, 0.2 µL of bovine serum albumin (BSA), and 10 ng of template DNA in a 20 µL reaction volume. The thermal cycling conditions were described previously (Wang et al 2022a). The PCR products were identified by 2% agarose gel electrophoresis, purified using an AxyPrep DNA gel extraction kit (Axygen, Corning, USA) and quantified using a QuantiFluor ™ -ST Blue Fluorescence Quantification System (Promega, Madison, USA).…”

Section: Methodsmentioning

confidence: 99%

Effects of biogas residue addition, as cultivation substrate, on ginseng growth

Gui,

Suo,

Qiu

et al. 2024

Plant Soil Environ.

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Energy shortages have become a major concern for people. With the advancements in science and technology, crop yields are increasing, and more crop straw is being produced. The annual straw production in China is approximately 700 million tons (Lin et al. 2022). In addition to being used as a substrate, fertiliser, fuel, animal feed and raw material, approximately 15% of the water is still unused (Zhou et al. 2023). The problem of environmental pollution caused by excess straw poses a serious threat to human health and has become a global problem that urgently needs to be solved. Straw energy conversion, such as converting straw into biogas through anaerobic fermentation, is one of the most effective ways to address residual straw pollution.During convergence, the production continuity determines that the emitted biogas residue is charac-terised by continuous, large amounts and incomplete material decomposition. Therefore, searching for efficient, convenient and low-cost methods is necessary to eliminate the subsequent pollution pressure caused by a large amount of excess biogas residue. Composting is a way to convert biogas residues into usable resources effectively. During the composting process, the undecomposed materials in the digestate are further degraded by microorganisms, and potential threats such as parasites, pathogens and weeds are significantly reduced (Bernal et al. 2009). Mature biogas residue compost has been used as a growing substrate for a variety of crops, such as zucchini, pepper, eggplant, lettuce, and tomato plants (Jara Samaniego et al. 2017, Meng et al. 2018, Liu et al. 2023a). However, perennial plants, particularly perennial medicinal plants, have rarely been reported.

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Inoculation with Penicillium citrinum aids ginseng in resisting Fusarium oxysporum by regulating the root and rhizosphere microbial communities

Cited by 10 publications

References 63 publications

Improper crop rotation may enrich soil‐borne pathogens of Panax notoginseng

Improper crop rotation may enrich soil‐borne pathogens of Panax notoginseng

Differentially enriched fungal communities in root rot resistant and susceptible varieties of tobacco (Nicotiana tabacum L.) under continuous monoculture cropping

Effects of biogas residue addition, as cultivation substrate, on ginseng growth

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