Inoculation with Xanthomonas oryzae pv. oryzae induces thylakoid membrane association of Rubisco activase in Oryza meyeriana

Yang, Yong; Chen, Yu; Wang, Xuming; Yan, Chengqi; Cheng, Ye; Chen, Jianping

doi:10.1016/j.jplph.2011.02.012

Cited by 11 publications

(12 citation statements)

References 21 publications

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“…Based on these results we suggest that the three QTLs identified in ASH1 were the ones transferred from O. meyeriana corresponding to the loci of the novel resistance genes in this wild rice. Our previous studies have revealed that the high resistance of O. meyeriana to rice BB disease results from multiple causes [34,35], which suggests that this wild rice may contain more than one gene conferring its strong resistance. This is consistent with the multiple resistance loci detected in ASH1 in this study.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Trait Loci Mapping for Bacterial Blight Resistance in Rice Using Bulked Segregant Analysis

Han

Yang

Wang

et al. 2014

IJMS

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Oryza meyeriana is highly resistant to rice bacterial blight (BB) and this resistance trait has been transferred to cultivated rice (O. sativa) using asymmetric somatic hybridization. However, no resistance genes have yet been cloned. In the present study, a progeny of the somatic hybridization with high BB resistance was crossed with a rice cultivar with high BB susceptibility to develop an F2 population. Using bulked segregant analysis (BSA), 17 polymorphic markers that were linked to rice BB resistance were obtained through scanning a total of 186 simple sequence repeats (SSR) and sequence-tagged site (STS) markers, evenly distributed on 12 chromosomes. A genetic linkage map was then constructed based on the 17 linkage markers and the F2 segregating population, which was followed by mapping for quantitative trait loci (QTLs) for BB resistance. Three QTLs were identified on chromosomes 1, 3 and 5, respectively, and the alleles of the resistant parent at any of the QTLs increased BB resistance. All of the three QTLs had a strong effect on resistance, explaining about 21.5%, 12.3% and 39.2% of the resistance variance, respectively. These QTLs were different from the loci of the BB resistance genes that have been identified in previous studies. The QTLs mapped in this work will facilitate the isolation of novel BB resistance genes and their utilization in rice resistance breeding.

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Section: Discussionmentioning

confidence: 99%

Quantitative Trait Loci Mapping for Bacterial Blight Resistance in Rice Using Bulked Segregant Analysis

Han

Yang

Wang

et al. 2014

IJMS

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“…After washing with phosphate buffer, the pieces were dehydrated in an ethanol series and embedded in resin (Lowicryl K4M) [27]. Ultra-thin sections were placed on nickel grids and incubated first for 5 min in distilled water, then for 30 min with blocking solution containing 0.05 M PBS with 1% BSA, 0.02% PEG20000, 0.1 M NaCl, and 1% NaN 3 .…”

Section: Methodsmentioning

confidence: 99%

Involvement of Multiple Types of Dehydrins in the Freezing Response in Loquat (Eriobotrya japonica)

Yang

Xie

et al. 2014

PLoS ONE

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Dehydrins (DHNs) are a family of plant proteins typically induced in response to stress conditions that cause cellular dehydration, such as low temperatures, high salinity, and drought. Loquat (Eriobotrya japonica) is a perennial fruit crop that blossoms during winter. Loquat fruitlets are frequently injured by freezing. To evaluate the role of the EjDHNs in freezing resistance in loquat fruitlets, two cultivars of loquat, the freezing-sensitive ‘Ninghaibai’ (FS-NHB) and the freezing-tolerant ‘Jiajiao’ (FT-JJ), were analyzed under induced freezing stress. Freezing stress led to obvious accumulation of reactive oxygen species and considerable lipid peroxidation in membranes during the treatment period. Both these phenomena were more pronounced in ‘FS-NHB’ than in ‘FS-JJ.’ Immunogold labeling of dehydrin protein was performed. DHN proteins were found to be concentrated mainly in the vicinity of the plasma membrane, and the density of the immunogold labeling was significantly higher after freezing treatment, especially in the more freezing-tolerant cultivar ‘FT-JJ.’ Seven DHNs, showing four different structure types, were obtained from loquat fruitlets and used to study the characteristics of different EjDHN proteins. These DHN proteins are all highly hydrophilic, but they differ significantly in size, ranging from 188 to 475 amino acids, and in biochemical properties, such as theoretical pI, aliphatic index, and instability index. Freezing treatment resulted in up-regulation of the expression levels of all seven EjDHNs, regardless of structure type. The accumulation of the transcripts of these EjDHN genes was much more pronounced in ‘FT-JJ’ than in ‘FS-NHB.’ Altogether, this study provides evidence that EjDHNs are involved in the cryoprotection of the plasma membrane during freeze-induced dehydration in loquat fruitlets.

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“…Yang et al . () reported that Xanthomonas oryzae pv. oryzae ( Xoo ) infection induced a changed distribution of Rubisco (ribulose‐1,5‐bisphosphate carboxylase/oxygenase) activase from the soluble stroma to the thylakoid membrane of chloroplasts.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the chloroplast constitutes a platform for the synthesis of important defence hormones, such as salicylic acid (SA) and jasmonic acid (JA), and both chloroplasts and mitochondria are essential providers of redox resources to fight pathogen attack. Yang et al (2011) reported that Xanthomonas oryzae pv. oryzae (Xoo) infection induced a changed distribution of Rubisco (ribulose-1,5-bisphosphate carboxylase/ oxygenase) activase from the soluble stroma to the thylakoid membrane of chloroplasts.…”

Section: Introductionmentioning

confidence: 99%

Loss of chloroplast‐localized protein phosphatase 2Cs in Arabidopsis thaliana leads to enhancement of plant immunity and resistance to Xanthomonas campestris pv. campestris infection

Akimoto-Tomiyama

Tanabe

Kajiwara

et al. 2017

Molecular Plant Pathology

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Protein phosphatases (PPs) counteract kinases in reversible phosphorylation events during numerous signal transduction pathways in eukaryotes. PP2Cs, one of the four major classes of the serine/threonine-specific PP family, are greatly expanded in plants. Thus, PP2Cs are thought to play a specific role in signal transduction pathways. Some rice PP2Cs classified in subgroup K are responsive to infection by the compatible Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight. In Arabidopsis thaliana, orthologous PP2C genes (AtPP2C62 and AtPP2C26) classified to subgroup K are also responsive to Xanthomonas campestris pv. campestris (Xcc, causal agent of black rot) infection. To elucidate the function of these subgroup K PP2Cs, atpp2c62- and atpp2c26-deficient A. thaliana mutants were characterized. A double mutant plant which was inoculated with a compatible Xcc showed reduced lesion development, as well as the suppression of bacterial multiplication. AtPP2C62 and AtPP2C26 localized to the chloroplast. Furthermore, the photosynthesis-related protein, chaperonin-60, was indicated as the potential candidate for the dephosphorylated substrate catalysed by AtPP2C62 and AtPP2C26 using two-dimensional isoelectric focusing sodium dodecylsulfate-polyacrylamide gel electrophoresis (2D-IDF-SDS-PAGE). Taken together, AtPP2C62 and AtPP2C26 are suggested to be involved in both photosynthesis and suppression of the plant immune system. These results imply the occurrence of crosstalk between photosynthesis and the plant defence system to control productivity under pathogen infection.

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Inoculation with Xanthomonas oryzae pv. oryzae induces thylakoid membrane association of Rubisco activase in Oryza meyeriana

Cited by 11 publications

References 21 publications

Quantitative Trait Loci Mapping for Bacterial Blight Resistance in Rice Using Bulked Segregant Analysis

Quantitative Trait Loci Mapping for Bacterial Blight Resistance in Rice Using Bulked Segregant Analysis

Involvement of Multiple Types of Dehydrins in the Freezing Response in Loquat (Eriobotrya japonica)

Loss of chloroplast‐localized protein phosphatase 2Cs in Arabidopsis thaliana leads to enhancement of plant immunity and resistance to Xanthomonas campestris pv. campestris infection

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