Inorganic and organic nitrogen uptake by phytoplankton and heterotrophic bacteria in the stratified Mid-Atlantic Bight

Bradley, Paul B.; Sanderson, Marta P.; Frischer, Marc E.; Brofft, Jennifer; Booth, Melissa G.; Kerkhof, Lee J.; Bronk, Deborah A.

doi:10.1016/j.ecss.2010.02.001

Cited by 81 publications

(42 citation statements)

References 66 publications

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“…Experimental evidence from field studies, however, is scarce, and most of it indicates that amino acids are not significantly used by phytoplankton (25,26,41). Our data contradict these previous observations and represent some of the first direct evidence of utilization of amino acids as an N source by a marine phytoplankton species in the environment (3,59).…”

Section: Discussioncontrasting

confidence: 57%

“…Despite these advances, direct measurements of uptake of specific forms of N by individual populations of phyto-and bacterioplankton are scarce. This is primarily due to the fact that most measurements of N uptake are made using glass fiber filters that collect autotrophs and some variable fraction of heterotrophic bacteria (3). Uptake rates thus represent bulk uptake by hundreds of different phytoplankton and bacterial species.…”

mentioning

confidence: 99%

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Use of Inorganic and Organic Nitrogen by Synechococcus spp. and Diatoms on the West Florida Shelf as Measured Using Stable Isotope Probing

Wawrik

Callaghan

Bronk

2009

Appl Environ Microbiol

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The marine nitrogen (N) cycle is a complex network of biological transformations in different N pools. The linkages among these different reservoirs are often poorly understood. Traditional methods for measuring N uptake rely on bulk community properties and cannot provide taxonomic information.15 N-based stable isotope probing (SIP), however, is a technique that allows detection of uptake of individual N sources by specific microorganisms. In this study we used 15 N SIP methodology to assess the use of different nitrogen substrates by Synechococcus spp. and diatoms on the west Florida shelf. Seawater was incubated in the presence of 15 N-labeled ammonium, nitrate, urea, glutamic acid, and a mixture of 16 amino acids. DNA was extracted and fractionated using CsCl density gradient centrifugation. Quantitative PCR was used to quantify the amounts of Synechococcus and diatom DNA as a function of density, and 15 N tracer techniques were used to measure rates of N uptake by the microbial community. The ammonium, nitrate, urea, and dissolved primary amine uptake rates were 0.077, 0. N-amino acids, but no evidence indicating uptake of labeled amino acids by diatoms was detected. These data suggest that N flow in communities containing Synechococcus spp. and diatoms has more plasticity than the new-versus-recycled production paradigm suggests and that these phytoplankters should not be viewed strictly as recycled and new producers, respectively.

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Section: Discussioncontrasting

confidence: 57%

mentioning

confidence: 99%

Use of Inorganic and Organic Nitrogen by Synechococcus spp. and Diatoms on the West Florida Shelf as Measured Using Stable Isotope Probing

Wawrik

Callaghan

Bronk

2009

Appl Environ Microbiol

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“…Urea is typically disregarded as an important N source to bacteria (Kirchman 2000), although several studies have found otherwise (Jørgensen 2006, Sanderson et al 2008, Bradley et al 2010b). In the present study, bacterial uptake represented ~50% of total urea uptake in the Ctrl.…”

Section: Phytoplankton Versus Bacterial Nitrogen Usementioning

confidence: 99%

Nitrogen uptake by phytoplankton and bacteria during an induced Phaeocystis pouchetii bloom, measured using size fractionation and flow cytometric sorting

Bradley¹,

Sanderson²,

Nejstgaard³

et al. 2010

Aquat. Microb. Ecol.

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“…However, these studies were not emphasized on the difference of NAB in the vertical dimension. A study showed bacterial nitrate assimilation comprises a greater proportion (up to 93 %) at the bottom of the Mid-Atlantic Bight [18]. However, information regarding NAB diversity in the twilight and aphotic zones is scarce, thereby limiting our understanding of nitrogen cycles in the water column.…”

Section: Introductionmentioning

confidence: 99%

Vertical Distribution of Bacterial Communities in the Indian Ocean as Revealed by Analyses of 16S rRNA and nasA Genes

Jiang

Jiao

2016

Indian J Microbiol

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Bacteria play an important role in the marine biogeochemical cycles. However, research on the bacterial community structure of the Indian Ocean is scarce, particularly within the vertical dimension. In this study, we investigated the bacterial diversity of the pelagic, mesopelagic and bathypelagic zones of the southwestern Indian Ocean (50.46°E, 37.71°S). The clone libraries constructed by 16S rRNA gene sequence revealed that most phylotypes retrieved from the Indian Ocean were highly divergent from those retrieved from other oceans. Vertical differences were observed based on the analysis of natural bacterial community populations derived from the 16S rRNA gene sequences. Based on the analysis of the nasA gene sequences from GenBank database, a pair of general primers was developed and used to amplify the bacterial nitrate-assimilating populations. Environmental factors play an important role in mediating the bacterial communities in the Indian Ocean revealed by canonical correlation analysis.

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Inorganic and organic nitrogen uptake by phytoplankton and heterotrophic bacteria in the stratified Mid-Atlantic Bight

Cited by 81 publications

References 66 publications

Use of Inorganic and Organic Nitrogen by Synechococcus spp. and Diatoms on the West Florida Shelf as Measured Using Stable Isotope Probing

Use of Inorganic and Organic Nitrogen by Synechococcus spp. and Diatoms on the West Florida Shelf as Measured Using Stable Isotope Probing

Nitrogen uptake by phytoplankton and bacteria during an induced Phaeocystis pouchetii bloom, measured using size fractionation and flow cytometric sorting

Vertical Distribution of Bacterial Communities in the Indian Ocean as Revealed by Analyses of 16S rRNA and nasA Genes

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