Inorganic carbon formation in rotating thin liquid films to support algal growth

Hajer, Ahmad Abu; Bayless, David J.

doi:10.1016/j.algal.2020.102109

Cited by 2 publications

(1 citation statement)

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“…We previously proposed that solid-surface culturing of Parachlorella kessleri was one of the most promising systems for algal culturing in view of its potential for high biomass production per unit area ( Miyauchi et al., 2020 ). However, P. kessleri cells in solid-surface culture, relative to those in liquid culture, showed that the rate of photosynthetic CO 2 fixation was lower in ordinary air (400 µmol CO 2 mol air −1 ) than in air enriched with CO 2 (2,000 µmol CO 2 mol air −1 ), which suggested a limitation of photosynthesis in solid-surface cells by CO 2 , which slowly dissolves and diffuses ( Hajer and Bayless, 2020 ). Additionally, light, nutrient, and water availability and the extent of dehydration on the solid surface were different from those in liquid, which might have affected the photosynthetic rate ( Blanken et al., 2014 ; Li et al., 2015 ; Lai et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Cellular response of Parachlorella kessleri to a solid surface culture environment

Miyauchi,

Ishikawa,

Hirakawa

et al. 2023

Front. Plant Sci.

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Attached culture allows high biomass productivity and is a promising biomass cultivating system because neither a huge facility area nor a large volume of culture medium are needed. This study investigates photosynthetic and transcriptomic behaviors in Parachlorella kessleri cells on a solid surface after their transfer from liquid culture to elucidate the physiological and gene-expression regulatory mechanisms that underlie their vigorous proliferation. The chlorophyll content shows a decrease at 12 h after the transfer; however, it has fully recovered at 24 h, suggesting temporary decreases in the amounts of light harvesting complexes. On PAM analysis, it is demonstrated that the effective quantum yield of PSII decreases at 0 h right after the transfer, followed by its recovery in the next 24 h. A similar changing pattern is observed for the photochemical quenching, with the PSII maximum quantum yield remaining at an almost unaltered level. Non-photochemical quenching was increased at both 0 h and 12 h after the transfer. These observations suggest that electron transfer downstream of PSII but not PSII itself is only temporarily damaged in solid-surface cells just after the transfer, with light energy in excess being dissipated as heat for PSII protection. It thus seems that the photosynthetic machinery acclimates to high-light and/or dehydration stresses through its temporal size-down and functional regulation that start right after the transfer. Meanwhile, transcriptomic analysis by RNA-Seq demonstrates temporary upregulation at 12 h after the transfer as to the expression levels of many genes for photosynthesis, amino acid synthesis, general stress response, and ribosomal subunit proteins. These findings suggest that cells transferred to a solid surface become stressed immediately after transfer but can recover their high photosynthetic activity through adaptation of photosynthetic machinery and metabolic flow as well as induction of general stress response mechanisms within 24 h.

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