Inorganic carbon stimulates the metabolic routes related to the polyhdroxybutyrate production in a Synechocystis sp. strain (cyanobacteria) isolated from wastewater

Rueda, Estel; Álvarez-González, Ana; Vilà, Joaquim; Díez-Montero, Rubén; Grifoll, Magdalena; García, Jesús Miguel Bairán

doi:10.1016/j.scitotenv.2022.154691

Cited by 13 publications

(10 citation statements)

References 53 publications

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“…This would explain the sustained intracellular PHB content after 7 days in starvation by the overexpression of phaC , with no observed PHB decrease. These findings agreed with other studies reporting conversion of glycogen to PHB in cyanobacteria 31,46,48 . The degradation of glycogen and its transformation into PHB occurs during prolonged N-starvation to mitigate the potential osmotic impacts of excessive intracellular metabolites accumulation and to generate ATP to maintain basic cellular functions 49,50 .…”

Section: Discussionsupporting

confidence: 93%

“…3B and Supplementary Fig. 12), indicating that glycogen was the initial storage compound synthesized in response to short-term macronutrient stress conditions, such as nitrogen depletion, as reported by other authors 30,[45][46][47] . Nevertheless, the higher expression of gltA on day 105 (Fig.…”

Section: Discussionsupporting

confidence: 79%

“…In repetition 4, samples were collected at the start (prior to Ac injection), the fourth day and at the end of the starvation phase. Methodology was adapted from 46 . Fresh biomass (10 mL) was harvested by centrifugation at 14,000 rpm for 5 min at 4 °C and stored at – 80 °C in an ultra-freezer (Arctiko, Denmark).…”

Section: Methodsmentioning

confidence: 99%

“…Gene expression levels were determined using the qPCR thermocycler Quantstudio 3 (ThermoFisher Scientific, USA). To do this, designed primers described in 46 at 300 nM and the Powerup SYBR master mix (ThermoFisher Scientific, USA) were used. The 16S RNA was selected as the housekeeping gene as the one with lower variability between the different tested conditions.…”

Section: Rna Extraction and Quantitative Real-time Pcrmentioning

confidence: 99%

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Bioplastic production by harnessing cyanobacteria-rich microbiomes for perpetual synthesis

Altamira-Algarra,

Lage,

Meléndez

et al. 2023

Preprint

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Departing from the conventional axenic and heterotrophic cultures, our research ventures into unexplored territory by investigating the potential of photosynthetic microbiomes for polyhydroxybutyrate (PHB) production. A cyanobacteria-rich microbiome was harnessed for PHB production in a 3 L photobioreactor under non-sterile conditions. The robust culture achieved up to 28 %dcwPHB over 108 days of alternating growth and accumulation phases. Nile Blue staining and Transmission Electron Microscope visualization successfully confirmed the presence of PHB granules within cyanobacteria cells. Analysis through proton Nuclear Magnetic Resonance further validated the extracted polymer as PHB. In addition, the overexpression of the enzyme PHA synthase throughout the accumulation phase correlated directly with the increased PHB production. Also, gene expression changes suggested that initially, glycogen served as the primary storage compound. However, with prolonged macronutrient stress, there was a shift of the carbon flux towards favoring PHB synthesis. Overall, it was demonstrated for the first time the feasibility of using a phototrophic microbiome to continuous production of PHB in a non-sterile system, contributing to advancing in the field of biopolymer production and offering valuable insights into the metabolic pathways involved.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Rna Extraction and Quantitative Real-time Pcrmentioning

confidence: 99%

See 2 more Smart Citations

Bioplastic production by harnessing cyanobacteria-rich microbiomes for perpetual synthesis

Altamira-Algarra,

Lage,

Meléndez

et al. 2023

Preprint

Self Cite

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“…R2020 (GenBank database access number: MN493570.1), which was isolated from a microalgaebased WWTP by direct streaking or serial dilution in agar plates (Rueda et al, 2020). The strain used in this study was selected for its adaptability to wastewater (as it was isolated from waste streams), and for its proven capability to accumulate high-value bioproducts as polyhydroxybutyrate (Rueda et al, 2022). The inoculum was maintained in 1 L Erlenmeyer flasks at 25 • C, using sterile BG11 medium.…”

Section: Cyanobacterium Inoculummentioning

confidence: 99%

Phycobiliprotein recovery coupled to the tertiary treatment of wastewater in semi-continuous photobioreactors. Tracking contaminants of emerging concern.

Bellver

Díez-Montero

Escolà

et al. 2023

Bioresource Technology

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Challenges, progress, and future perspectives for cyanobacterial polyhydroxyalkanoate production

Rueda,

Gonzalez-Flo,

Mondal

et al. 2024

Rev Environ Sci Biotechnol

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Polyhydroxyalkanoates (PHA) are a promising bio-based alternative to traditional plastics derived from petroleum. Cyanobacteria are photosynthetic organisms that produce PHA from CO2 and sunlight, which can potentially reduce production costs and environmental footprint in comparison to heterotrophic bacteria cultures because (1) they utilize inorganic carbon sources for growth and (2) they do not require intensive aeration for oxygenation. Moreover, supplementing precursors such as propionate, acetate, valerate, etc., can be used to obtain various copolymers with plastic customizable properties in comparison to the classical homopolymers, such as polyhydroxybutyrate, PHB. This critical review covers the latest advances in PHA production, including recent discoveries in the metabolism interplay between PHA and glycogen production, and new insights into cultivation strategies that enhance PHA accumulation, and purification processes. This review also addresses the challenges and suggests potential solutions for a viable industrial PHAs production process.

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Inorganic carbon stimulates the metabolic routes related to the polyhdroxybutyrate production in a Synechocystis sp. strain (cyanobacteria) isolated from wastewater

Cited by 13 publications

References 53 publications

Bioplastic production by harnessing cyanobacteria-rich microbiomes for perpetual synthesis

Bioplastic production by harnessing cyanobacteria-rich microbiomes for perpetual synthesis

Phycobiliprotein recovery coupled to the tertiary treatment of wastewater in semi-continuous photobioreactors. Tracking contaminants of emerging concern.

Challenges, progress, and future perspectives for cyanobacterial polyhydroxyalkanoate production

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