Inorganic Carbon Transport and Fixation in Cells of Anabaena variabilis Adapted to Mixotrophic Conditions

Nieva, M.; Valiente, Eduardo Fernández

doi:10.1093/oxfordjournals.pcp.a028905

Cited by 12 publications

(9 citation statements)

References 35 publications

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“…Vonshak et al (2000) reported that assimilation of organic carbon in mixotrophic culture changes respiratory as well as photosynthetic metabolism of microalgae. Marquez- Rocha et al (1993) showed that the observed mixotrophic growth was the sum of autotrophic and heterotrophic metabolisms that operated independently, however Nieva and Valiente (1996), reported that the rate of CO 2 fixation was lower in mixotrophic culture, which suggested that autotrophic growth might be modulated in mixotrophic culture. Yang et al (2000) found that for Chlorella pyrenoidosa, theoretical yields of biomass on ATP was the highest for heterotrophic culture (19 g mol -1 ).…”

Section: Biomass Yield From Glucosementioning

confidence: 99%

Evaluation of growth yield of Spirulina (Arthrospira) sp. in photoautotrophic, heterotrophic and mixotrophic cultures

Chojnacka

Zielińska

2011

World J Microbiol Biotechnol

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In microbial cultures, both cellular growth rate and yield (defined as the degree of substrate conversion into the biomass) are important. Although effect of culture conditions on growth kinetics has been well documented for various microbial strains, there is almost no literature concerning the effect of environmental conditions on growth equilibrium, expressed as biomass yield coefficients from substrate. The present paper discusses the effect of culture conditions: irradiance (physical substrate) and glucose concentration (chemical substrate) on biomass yield coefficients from two chemical substrates: glucose and nitrate-nitrogen in photoautotrophic, heterotrophic and mixotrophic culture of blue-green alga Spirulina (Arthrospira) sp. The efficiency of substrates incorporation into the biomass can be precisely determined only if the elemental composition of the biomass is known. The experimental results showed that culture conditions had a substantial influence on biomass yield coefficients (biomass yield from glucose and nitrate-nitrogen). It was found that, the increase of irradiance favoured increase of biomass yield coefficient from both, glucose and nitrate-nitrogen. However, in the case of yield from nitrogen in mixotrophic culture, the effect was opposite. The effect of glucose concentration was different: the higher the initial glucose concentration, the lower the biomass yield coefficients from chemical substrates.

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Section: Biomass Yield From Glucosementioning

confidence: 99%

Evaluation of growth yield of Spirulina (Arthrospira) sp. in photoautotrophic, heterotrophic and mixotrophic cultures

Chojnacka

Zielińska

2011

World J Microbiol Biotechnol

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“…In long-term, darkgrown, fructose-adapted cells, there is an increase in photosystem II, resulting in a decrease in the ratio of photosystem I to photosystem II (23). Cells grown with low CO 2 in the presence of fructose do not fix CO 2 well because of decreased carbonic anhydrase and decreased ribulose bis-phosphate carboxylase oxygenase (29). The decrease in oxygen production in fructosegrown cells is thought to contribute to a micro-oxic environment that better supports nitrogen fixation (14).…”

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confidence: 99%

Regulation of Fructose Transport and Its Effect on Fructose Toxicity in Anabaena spp

2008

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Anabaena variabilis grows heterotrophically using fructose, while the close relative Anabaena sp. strain PCC 7120 does not. Introduction of a cluster of genes encoding a putative ABC transporter, herein named frtRABC, into Anabaena sp. strain PCC 7120 on a replicating plasmid allowed that strain to grow in the dark using fructose, indicating that these genes are necessary and sufficient for heterotrophic growth. FrtR, a putative LacI-like regulatory protein, was essential for heterotrophic growth of both cyanobacterial strains. Transcriptional analysis revealed that the transport system was induced by fructose and that in the absence of FrtR, frtA was very highly expressed, with or without fructose. In the frtR mutant, fructose uptake was immediate, in contrast to that in the wild-type strain, which required about 40 min for induction of transport. In the frtR mutant, high-level expression of the fructose transporter resulted in cells that were extremely sensitive to fructose. Even in the presence of the inducer, fructose, expression of frtA was low in the wild-type strain compared to that in the frtR mutant, indicating that FrtR repressed the transporter genes even in the presence of fructose. FrtR bound to the upstream region of frtA, but binding was not visibly altered by fructose, further supporting the hypothesis that fructose has only a modest effect in relieving repression of frtA by FrtR. A. variabilis grew better with increasing concentrations of fructose up to 50 mM, showing increased cell size and heterocyst frequency. Anabaena sp. strain PCC 7120 did not show any of these changes when it was grown with fructose. Thus, although Anabaena sp. strain PCC 7120 could take up fructose and use it in the dark, fructose did not improve growth in the light.

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“…It is possible to detect the A. variabilis cells down to 2.45 mg/L. This amount is equivalent to 3.5 × 10 5 cells/mL . Jeon et al .…”

Section: Methodsmentioning

confidence: 99%

Photoelectrochemical Coulometric Sensing of Anabaena variabilis Through a Mediated Electron Transfer System

Cho

Lee

Kim

2019

Bulletin Korean Chem Soc

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Inorganic Carbon Transport and Fixation in Cells of Anabaena variabilis Adapted to Mixotrophic Conditions

Cited by 12 publications

References 35 publications

Evaluation of growth yield of Spirulina (Arthrospira) sp. in photoautotrophic, heterotrophic and mixotrophic cultures

Evaluation of growth yield of Spirulina (Arthrospira) sp. in photoautotrophic, heterotrophic and mixotrophic cultures

Regulation of Fructose Transport and Its Effect on Fructose Toxicity in Anabaena spp

Photoelectrochemical Coulometric Sensing of Anabaena variabilis Through a Mediated Electron Transfer System

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