(Inorganic Nanofiber/Enzyme) Hybrid Hydrogel: Preparation, Characterization, and Enzymatic Activity of Imogolite/Pepsin Conjugate

Inoue, Nozomi; Otsuka, Hideyuki; Wada, Shin Ichiro; Takahara, Atsushi

doi:10.1246/cl.2006.194

Cited by 35 publications

(28 citation statements)

References 13 publications

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“…A hybrid hydrogel composed of imogolite and pepsin is formed by the interaction between the Al-OH group of imogolite and the phosphoric group of pepsin [48]. Accordingly, LbL assembly in the present method can be applied to the formation of a heterogeneous catalytic system with a macroporous architecture.…”

Section: Uniqueness Of Materials Prepared By Lbl Assembly Of Imogolitmentioning

confidence: 97%

“…Macroporous hollow spheres were formed through the removal of polystyrene cores. To the best of our knowledge, this is the first report on the use of imogolite for LbL assembly, although imogolite/pepsin conjugate [48] and microtubes composed of imogolite and polyelectrolytes fabricated using ionic interactions of imogolite [49] have been reported recently.…”

Section: Introductionmentioning

confidence: 99%

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Layer-by-layer assembly of imogolite nanotubes and polyelectrolytes into core-shell particles and their conversion to hierarchically porous spheres

Kuroda

2008

Science and Technology of Advanced Materials

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Core-shell particles were prepared by the layer-by-layer (LbL) assembly of imogolite (IMO) nanotubes and poly(sodium 4-styrenesulfonate) (PSS) on polystyrene particles (diameter: 800 nm) coated preliminarily with poly(diallyldimethylammonium chloride) (PDDA). PSS and imogolite were alternately adsorbed on the particles to form core-shell particles with one to three bilayers of PSS/IMO. Macroporous hollow spheres were formed by removing polystyrene cores via heat treatment or extraction when the number of bilayers was 2 or 3. The sample formed by extraction (the number of bilayer was 3) showed only macroporosity and PSS remained in the shell, whereas the heat-treated sample showed hierarchical micro-and macroporosities. When the diameter of polystyrene particles decreased from 800 nm to 300 or 100 nm, hollow spheres were deformed because of the increase in the relative length of imogolite nanotubes against the size of polystyrene particles. Imogolite is a promising building block of hierarchically porous materials with core-shell morphologies using LbL assembly.

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Section: Uniqueness Of Materials Prepared By Lbl Assembly Of Imogolitmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Layer-by-layer assembly of imogolite nanotubes and polyelectrolytes into core-shell particles and their conversion to hierarchically porous spheres

Kuroda

2008

Science and Technology of Advanced Materials

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“…Takahara et al have reported on the encapsulation of the hydrolytic enzyme, pepsin, and deoxyribonucleic acid in an ASNT hydrogel. [23][24][25] Within the ASNT hydrogel, pepsin was evenly dispersed throughout. Compared to the free enzyme in solution, pepsin immobilized within the hydrogel can be easily recovered from the reaction system, and can be used repeatedly.…”

Section: Introductionmentioning

confidence: 99%

Effective encapsulation of laccase in an aluminium silicate nanotube hydrogel

et al. 2014

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Aluminium silicate nanotubes (ASNT), with a length to width ratio of four, were synthesized from aluminium chloride and sodium silicate, and the ASNT hydrogel was easily prepared by adjusting the pH to 7. The hydrogel nanotube concentration was 1.5 wt%. Laccase, a type of oxidase, was encapsulated during ASNT hydrogel formation. This encapsulation method will have fewer negative effects on the relatively unstable enzyme because of the milder conditions used, which are different from sol-gel silica formation by acidic catalysis using strong acids such as hydrochloric acid. The obtained hydrogels were fully characterized by various methods such as field-emission scanning electron microscopy and Fourier transform infrared spectroscopy. The ASNT-hydrogel encapsulated enzyme worked well; notably, laccase-ASNT hydrogels prepared from the shortest nanotubes exhibited a higher activity than the free laccase in solution because of an improvement in the substrate affinity of the encapsulated enzyme. Tryptophan fluorescence spectroscopy indicated that the highly ordered structure of laccase was not altered once bound to the nanotubes within the hydrogel. Notably, after nine repeated reactions, laccase encapsulated in the ASNT hydrogel retained its activity. The cycle performance of the encapsulated enzyme indicates that no enzyme was released from the ASNT hydrogel. In addition, the laccase-ASNT hydrogel was easily used to prepare transparent thin films on glass cover slips, while still maintaining the enzyme activity. Another oxidase, myoglobin, was also encapsulated in the same type of ASNT hydrogel.Although free myoglobin in solution demonstrated oxidation activity, the activity of the bound protein was remarkably decreased due to changes in its tertiary structure when inside the ASNT hydrogel.

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“…Since a synthetic pathway was established, imogolite is steadily available 22, 23. Although imogolite has an interesting nanostructure, there are only a few reports about its interaction with biopolymers 24–26. Hirai et al investigated the liquid crystal structure in the mixture of tunicate cellulose and imogolite in aqueous solution and prepared the nanocomposite film with high modulus 24.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of complex gel of type I collagen and aluminosilicate containing imogolite nanofibers

Nakano

Teramoto

Chen

et al. 2010

J of Applied Polymer Sci

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Complex gel materials of Type I collagen and aluminosilicate containing imogolite nanofibers were prepared as opaque gel by mixing an acidic fine dispersion of aluminosilicate with an acidic solution of collagen. The product was stained blue by Coomassie Brilliant Blue (CBB), indicating that the gel contained collagen. A white sponge was obtained after lyophilization of the complex gel. Elemental analysis revealed that the complex contains C, H, N, Al, and Si atoms; and the compositional ratio of aluminosilicate/collagen (w/w) was calculated as 0.75 for the complex gel when aluminosilicate was mixed with an equal quantity of collagen.Transmission electron microscope (TEM) observation showed that aluminosilicate nanofibers were homogeneously distributed in the collagen matrix. The thermogravimetric analysis (TGA) curve of the complex was not a simple summation of each components, and especially, the weight loss step corresponding to detachment of the adsorbed water observed in aluminosilicate became difficult to distinguish, suggesting that the adsorbed water was removed in the complexation.

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(Inorganic Nanofiber/Enzyme) Hybrid Hydrogel: Preparation, Characterization, and Enzymatic Activity of Imogolite/Pepsin Conjugate

Cited by 35 publications

References 13 publications

Layer-by-layer assembly of imogolite nanotubes and polyelectrolytes into core-shell particles and their conversion to hierarchically porous spheres

Layer-by-layer assembly of imogolite nanotubes and polyelectrolytes into core-shell particles and their conversion to hierarchically porous spheres

Effective encapsulation of laccase in an aluminium silicate nanotube hydrogel

Preparation and characterization of complex gel of type I collagen and aluminosilicate containing imogolite nanofibers

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