Inorganic nitrogen dynamics in intertidal rocky biofilms and sediments of the Douro River estuary (Portugal)

Magalhães, Catarina; Wiebe, W. J.; Joye, Samantha B.; Bordalo, Adriano A.

doi:10.1007/bf02696070

Cited by 33 publications

(33 citation statements)

References 58 publications

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“…Taking its potential N 2 O emission rate of 0.068 nmol ind. , which is on the same order of magnitude as the benthic N 2 O fluxes reported for estuarine intertidal sediments (Middelburg et al 1995) and intertidal rocky biofilms (Magalhaes et al 2005).…”

Section: Correlation With Species Traitssupporting

confidence: 77%

“…Microbial nitrogen conversions and concomitant N 2 O production are especially stimulated in coastal sediments and in rock biofilms, due to high riverine input of nitrogen (Seitzinger & Nixon 1985, Law et al 1992, Middelburg et al 1995, Robinson et al 1998, Magalhaes et al 2005. Nitrification activity prevails at the oxic sediment surface and is fuelled by ammonium from organic matter degradation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nitrous oxide production associated with coastal marine invertebrates

Heisterkamp¹,

Schramm²,

Beer³

et al. 2010

Mar. Ecol. Prog. Ser.

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Section: Correlation With Species Traitssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Nitrous oxide production associated with coastal marine invertebrates

Heisterkamp¹,

Schramm²,

Beer³

et al. 2010

Mar. Ecol. Prog. Ser.

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“…1), indicating that ammonium availability had a stimulating effect on both the establishment and the intensity of biofilm-associated nitrification activity, as previously reported (3). Maximum rates were similar to activities measured at the oxic/anoxic interface of river sediments (18 to 45 nmol cm Ϫ2 h Ϫ1 ) (7) but in the lower range of activities reported from river and estuary biofilms (10 to 171 nmol cm Ϫ2 h Ϫ1 ) (13,19,20,38). Community composition and abundance of ammonia oxidizers.…”

supporting

confidence: 80%

Ammonium Availability Affects the Ratio of Ammonia-Oxidizing Bacteria to Ammonia-Oxidizing Archaea in Simulated Creek Ecosystems

Herrmann

Scheibe

Avrahami

et al. 2011

Appl Environ Microbiol

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The ammonia-oxidizing microbial community colonizing clay tiles in flow channels changed in favor of ammonia-oxidizing bacteria during a 12-week incubation period even at originally high ratios of ammoniaoxidizing archaea to ammonia-oxidizing bacteria (AOB). AOB predominance was established more rapidly in flow channels incubated at 350 M NH 4 ؉ than in those incubated at 50 or 20 M NH 4 ؉ . Biofilm-associated potential nitrification activity was first detected after 28 days and was positively correlated with bacterial but not archaeal amoA gene copy numbers.Nitrogen transformation processes in small-river ecosystems are primarily associated with the sediment or epilithic biofilms (4,5,18). Here, nitrification as the first step in coupled nitrification-denitrification is a key step in the removal of the allochthonous nitrogen load, preventing the eutrophication of downstream ecosystems (1,5,29,39). The activity and community composition of biofilm-associated nitrifying microorganisms have been investigated extensively in wastewater treatment systems (27,36), in drinking water distribution systems (17, 41), and in lakes, large rivers, and estuaries (7,19,21,39) but only rarely in small-creek ecosystems. The first and ratelimiting step in nitrification, oxidation of ammonia, is carried out by ammonia-oxidizing archaea (AOA) (14) and ammoniaoxidizing bacteria (AOB) (16). Molecular surveys targeting the amoA gene, which encodes catalytic subunit A of ammonia monooxygenase, the key enzyme of ammonia oxidation (33), as well as cultivation-based studies, indicated that pH (26), salinity (25, 34), and especially ammonium availability (6, 22) could play important roles in the ecological niche differentiation of AOA and AOB. However, only little is known about archaeal versus bacterial ammonia oxidizers in creek or river ecosystems or about the association of AOA with biofilms (37,43). In this study, we used flow channels (FC) as simulated creek ecosystems to investigate the influence of ammonium availability (i) on the establishment of biofilm-associated nitrification activity, (ii) on the community composition of biofilm-associated AOA and AOB, and (iii) on temporal patterns of the abundance of AOA and AOB during a 12-week incubation period.FC experiment. Water for FC was obtained from three shell limestone creeks (Ammerbach, Leutra South, and Leutra North) located near the city of Jena (Thuringia, Germany). The creeks differed in their nutrient concentrations, with the highest nutrient load at Ammerbach resulting from diffuse wastewater inputs (Table 1). Flow velocities ranged from 0.105 Ϯ 0.092 to 0.230 Ϯ 0.224 m s Ϫ1 . Plexiglas FC measuring 160 by 10 by 18 cm (see Fig. S1 in the supplemental material) were set up in triplicate for each creek. Concentrations of NH 4 ϩ in the water phase of the FC were adjusted to 350 mol liter Ϫ1 (Ammerbach; FC 1 to 3), 50 mol liter Ϫ1 (Leutra South, FC 4 to 6), and 20 mol liter Ϫ1 (Leutra North, FC 7 to 9) to run the experiment at three distinct levels of NH 4 ϩ . pHs and concentrat...

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“…4C). This is not the case for animals colonizing hard substrates or the sediment surface (e.g., snails and bivalves on rocky shores), and we suggest that the very high nitrous oxide fractions and emissions measured at intertidal hard substrate sites (25) could be due to gut denitrification of the epifauna. The rate of nitrous oxide emission from benthic invertebrates may thus not only depend on the rate of nitrous oxide production in their gut but also on the site of the animal habitat relative to the sediment-water interface (5).…”

Section: Grazersmentioning

confidence: 67%

Nitrous oxide emission by aquatic macrofauna

Stief¹,

Poulsen²,

Nielsen³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

139

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A large variety of aquatic animals was found to emit the potent greenhouse gas nitrous oxide when nitrate was present in the environment. The emission was ascribed to denitrification by ingested bacteria in the anoxic animal gut, and the exceptionally high N2O-to-N2 production ratio suggested delayed induction of the last step of denitrification. Filter-and deposit-feeding animal species showed the highest rates of nitrous oxide emission and predators the lowest, probably reflecting the different amounts of denitrifying bacteria in the diet. We estimate that nitrous oxide emission by aquatic animals is quantitatively important in nitraterich aquatic environments like freshwater, coastal marine, and deep-sea ecosystems. The contribution of this source to overall nitrous oxide emission from aquatic environments might further increase because of the projected increase of nitrate availability in tropical regions and the numeric dominance of filter-and depositfeeders in eutrophic ecosystems.aquatic animals ͉ eutrophication ͉ sediment ͉ gut microbiology ͉ denitrification

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Inorganic nitrogen dynamics in intertidal rocky biofilms and sediments of the Douro River estuary (Portugal)

Cited by 33 publications

References 58 publications

Nitrous oxide production associated with coastal marine invertebrates

Nitrous oxide production associated with coastal marine invertebrates

Ammonium Availability Affects the Ratio of Ammonia-Oxidizing Bacteria to Ammonia-Oxidizing Archaea in Simulated Creek Ecosystems

Nitrous oxide emission by aquatic macrofauna

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