Inorganic Phosphate Absorption in Small Intestine

Danisi, Giustina; Murer, Heini

doi:10.1002/cphy.cp060412

Cited by 29 publications

(29 citation statements)

References 105 publications

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“…This raises a possibility of a presence of an inhibitor of 1-hydroxylase in post-renal transplant patients. Low 1,25(OH) 2 D may potentially impair renal phosphorus reabsorption, blunt intestinal phosphorus absorption, and contribute to the phosphorus depletion [27,61,62]. At the present time, however, the paucity of data precludes a definitive conclusion regarding the role of 1,25(OH) 2 D in PRT phosphorus wasting.…”

Section: 25-dihydroxyvitamin-dmentioning

confidence: 91%

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Post-renal transplantation hypophosphatemia: a review and novel insights

Ghanekar

Welch

Moe³

et al. 2006

Current Opinion in Nephrology and Hypertension

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Section: 25-dihydroxyvitamin-dmentioning

confidence: 91%

“…1) [24]. Intestinal phosphorus absorption occurs mainly via a nonsaturable process and is not significantly influenced by the known regulators of phosphorus homeostasis [24,27,28]. The kidney plays a pivotal role in the regulation of phosphorus homeostasis.…”

Section: Normal Phosphorus Homeostasismentioning

confidence: 99%

Post-renal transplantation hypophosphatemia: a review and novel insights

Ghanekar

Welch

Moe³

et al. 2006

Current Opinion in Nephrology and Hypertension

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“…Both of these studies suggested that hagfish nutrient transport systems are more similar to those of mammals than to those of teleost fish. With regard to intestinal P i uptake, hagfish appear to be similar to both trout and mammals (11), primarily due to the high P i concentrations presumably present in the intestine after feeding. However, further physiological and molecular characterization is required to confirm this.…”

Section: Discussionmentioning

confidence: 95%

“…We therefore investigated the role of the hagfish Slc34a2-like transporter in P i uptake using a pyrophosphate structural analog and putative Slc34a competitive inhibitor, PFA (25). We also investigated Na ϩ -dependent P i uptake in a Na ϩ -limiting environment, as Slc34a has been demonstrated previously to be a Na ϩ -dependent transporter in trout (2) and other higher vertebrates (11,42).…”

Section: Discussionmentioning

confidence: 99%

Phosphate absorption across multiple epithelia in the Pacific hagfish (Eptatretus stoutii)

Schultz

Guffey

Clifford

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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Inorganic phosphate (Pi) is an essential nutrient for all organisms, but in seawater, Pi is a limiting nutrient. This study investigated the primary mechanisms of Pi uptake in Pacific hagfish ( Eptatretus stoutii) using ex vivo physiological and molecular techniques. Hagfish were observed to have the capacity to absorb Pi from the environment into at least three epithelial surfaces: the intestine, skin, and gill. Pi uptake in all tissues was concentration dependent, and saturable Pi transport was observed in the skin and gill at <2.0 mmol/l Pi. Gill and intestinal Pi uptake was sodium dependent, but Pi uptake into the skin increased under low sodium conditions. Gill Pi transport exhibited an apparent affinity constant ∼0.23–0.6 mmol/l Pi. A complete sequence of a type II sodium phosphate cotransporter (Slc34a) was obtained from the hagfish gill. Phylogenetic analysis of the hagfish Slc34a transporter indicates that it is earlier diverging than, and/or ancestral to, the other identified vertebrate Slc34a transporters (Slc34a1, Slc34a2, and Slc34a3). With the use of RT-PCR, the hagfish Slc34a transcript was detected in the intestine, skin, gill, and kidney, suggesting that this may be the transporter involved in Pi uptake into multiple epithelia in the hagfish. This is the first measurement of Pi uptake across the gill or skin of any vertebrate animal and first sodium phosphate cotransporter identified in hagfish.

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“…The cells were then stimulated with 100 µM K-Pi prepared in the corresponding NaCl-choline chloride dilution buffers, and the DMR response was recorded for an additional 65 min. NaHCO 3 was not included in the assay buffer for the sodium-dependent studies so that the various sodium concentrations could be accounted for accurately.…”

Section: Measurement Of Epic Dmr Responsementioning

confidence: 99%

Development of a Label-free Assay for Sodium-Dependent Phosphate Transporter NaPi-IIb

et al. 2012

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The most widely used assay format for characterizing plasma membrane transporter activity measures accumulation of radiolabeled substrates in tissues or cells expressing the transporters. This assay format had limitations and disadvantages; therefore, there was an unmet need for development of a homogeneous, nonradioactive assay for membrane transporter proteins. In this report, the authors describe the development of a label-free homogeneous assay for the sodium-dependent phosphate transporter NaPi-IIb using the Epic system. The addition of phosphate stimulated a dynamic mass redistribution (DMR) profile unique to cells expressing NaPi-IIb but not on parental cells. This DMR profile was phosphate specific because sulfate or buffer alone did not elicit the same response. Furthermore, the DMR response observed was phosphate and sodium dependent, with Km values in the micromolar and millimolar range, respectively. A known NaPi-IIb noncompetitive inhibitor was shown to completely inhibit the phosphate-stimulated DMR response, suggesting that this observed DMR response is an NaPi-IIb-mediated cellular event. The results demonstrate that a novel label-free assay was developed for studying transporter-mediated cellular activity, and this DMR assay platform could be applicable to other membrane transporter proteins.

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Inorganic Phosphate Absorption in Small Intestine

Cited by 29 publications

References 105 publications

Post-renal transplantation hypophosphatemia: a review and novel insights

Post-renal transplantation hypophosphatemia: a review and novel insights

Phosphate absorption across multiple epithelia in the Pacific hagfish (Eptatretus stoutii)

Development of a Label-free Assay for Sodium-Dependent Phosphate Transporter NaPi-IIb

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