Inorganic polyphosphate from the immunobiotic Lactobacillus rhamnosus CRL1505 prevents inflammatory response in the respiratory tract

Deza, María Alejandra Correa; Olmos, Antonieta Rodríguez de; Suárez, Nadia E.; Valdez, Graciela Font de; Salva, Susana; Gerez, Carla Luciana

doi:10.1016/j.sjbs.2021.06.010

Cited by 10 publications

(7 citation statements)

References 57 publications

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“…The study of poly-P production in lactobacilli, bifidobacteria and probiotics in general received low attention until this molecule was reported as a functional bacterial metabolite 10 . Since then, probiotic-derived poly-P has been characterized as a beneficial molecule that protects against intestinal injury 7 , 9 , but additional functions in preventing inflammatory processes at different locations are also being reported 39 , 40 . In this study, we show that, under the tested conditions with our collection of probiotics, production of poly-P was highly variable among strains.…”

Section: Discussionmentioning

confidence: 99%

Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042

Alcántara

Pérez

Huedo

et al. 2023

Sci Rep

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Polyphosphate (poly-P) biosynthesis in bacteria has been linked to many physiological processes and has been characterized as an interesting functional molecule involved in intestinal homeostasis. We determined the capacity for poly-P production of 18 probiotic strains mainly belonging to Bifidobacterium and former Lactobacillus genera, showing that poly-P synthesis varied widely between strains and is dependent on the availability of phosphate and the growth phase. Bifidobacteria were especially capable of poly-P synthesis and poly-P kinase (ppk) genes were identified in their genomes together with a repertoire of genes involved in phosphate transport and metabolism. In Bifidobacterium longum KABP042, the strain we found with highest poly-P production, variations in ppk expression were linked to growth conditions and presence of phosphate in the medium. Moreover, the strain produced poly-P in presence of breast milk and lacto-N-tetraose increased the amount of poly-P synthesized. Compared to KABP042 supernatants low in poly-P, exposure of Caco-2 cells to KABP042 supernatants rich in poly-P resulted in decreased epithelial permeability and increased barrier resistance, induction of epithelial protecting factors such as HSP27 and enhanced expression of tight junction protein genes. These results highlight the role of bifidobacteria-derived poly-P as a strain-dependent functional factor acting on epithelial integrity.

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Section: Discussionmentioning

confidence: 99%

Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042

Alcántara

Pérez

Huedo

et al. 2023

Sci Rep

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“…Previous results revealed that Lr-CRL1505 has multiple functions, for instance, as a probiotic can enhance immune responses in the gut (Salva et al 2010 ) and provide protection against bacterial respiratory pathogens, respiratory syncytial virus, and influenza virus (Zelaya et al 2015 , 2016 ; Tonetti 2020 ). Furthermore, its intracellular extract with polyP (as a postbiotic) was able to prevent the development of local inflammatory response in the respiratory mucosa in a murine model of acute respiratory inflammation (Correa Deza et al 2021a ), a fact that allows new applications of Lr-CRL1505 as a probiotic and postbiotic. In this work, the effect of culture parameters (pilot scale biomass production, pH and growth phase) on cell viability, heat tolerance and polyP accumulation by Lr-CRL1505 was evaluated in order to establish better conditions to obtain, on the one hand, heat-tolerant biomass with a high percentage of live cells and, on the other hand, intracellular extracts with high polyP levels, depending on the destination of the functional product (probiotic or postbiotic).…”

Section: Discussionmentioning

confidence: 99%

“…S1). By DAPI-based fluorescence technique, there is a threshold below which short polyP chains are not detectable, however, the polyP chain length from Lr-CRL1505 was previously reported greater than 45 phosphate residues (Correa Deza et al 2021a ).…”

Section: Methodsmentioning

confidence: 99%

“…Lr-CRL1505 administrated as alive cells improved the immune responses in the gut and beyond the gastrointestinal tract (Salva et al 2010 ) also being effective against bacterial respiratory pathogens, respiratory syncytial, and influenza virus (Chiba et al 2013 ; Herrera et al 2014 ; Tomosada et al 2013 ; Tonetti et al 2020 ; Zelaya et al 2015 ; 2016 ). On the other hand, the intracellular accumulation of polyP granules by Lr-CRL1505 was demonstrated and its intracellular extract containing this biopolymer showed an anti-inflammatory effect when it was nasally administered to a mouse model of acute respiratory inflammation (Correa Deza et al 2021a ). These results support the potential of the polyP from Lr-CRL1505 as a new postbiotic product to complement the treatment of respiratory diseases.…”

Section: Introductionmentioning

confidence: 99%

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Effect of culture parameters on the heat tolerance and inorganic polyphosphate accumulation by Lacticaseibacillus rhamnosus CRL1505, a multifunctional bacterium

Deza

Salva

Grillo-Puertas

et al. 2023

World J Microbiol Biotechnol

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Lacticaseibacillus rhamnosus CRL1505 can be used in functional products as a probiotic powder (dried live cells) or as a postbiotic intracellular extract containing inorganic polyphosphate as a functional biopolymer. Thus, the aim of this work was to optimize the production of Lr-CRL1505 depending on the target of the functional product (probiotic or postbiotic). For this purpose, the effect of culture parameters (pH, growth phase) on cell viability, heat tolerance and polyphosphate accumulation by Lacticaseibacillus rhamnosus CRL1505 was evaluated. Fermentations at free pH produced less biomass (0.6 log units) than at controlled pH while the growth phase affected both polyphosphate accumulation and cell heat tolerance. Exponential phase cultures showed 4–15 times greater survival rate against heat shock and 49–62% increased polyphosphate level, compared with the stationary phase. Results obtained allowed setting the appropriate culture conditions for the production of this strain according to its potential application, i.e., as live probiotic cells in powder form or postbiotic. In the first case, running fermentations at pH 5.5 and harvesting the cells at the exponential phase are the best conditions for obtaining a high live biomass yield capable of overcoming heat stress. Whereas the postbiotic formulations production requires fermentations at free pH and harvesting the cells in exponential phase to increase the intracellular polyphosphate level as a first step. Supplementary Information The online version contains supplementary material available at 10.1007/s11274-023-03625-0.

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“…Subsequent research has revealed that poly-P derived from probiotics offers several benefits, such as protecting against intestinal injury (Kashima et al 2015, Fujiya et al 2020. Additionally, there are emerging reports suggesting its role in preventing inflammatory processes at various locations (Deza et al 2021, Takauji et al 2021. While poly-P is not actively transported, trace levels can be detected outside the cells (Saiki et al 2016).…”

Section: Ache/bche Activity Determination and Inhibition Studiesmentioning

confidence: 99%

Effects of p-coumaric acid on Probiotic Properties of Lactobacillus acidophilus LA-5 and Lacticaseibacillus rhamnosus GG

DEREBASİ,

BULUT,

ERDEN

et al. 2023

Preprint

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p-coumaric acid (p-CA) is one of the most abundant phenolic compounds in nature and human diet. Probiotic bacteria are microorganisms conferring health benefits on host when administered in adequate amounts. The synbiotic interaction of these two contents, found in gastrointestinal tract, and effect of this interaction on intestines are not known. Thus, this study aimed to investigate synbiotic relationship between p-CA and Lactobacillus acidophilus LA-5 (LA-5), Lacticaseibacillus rhamnosus GG (LGG). They were grown in the presence of different concentrations of p-CA and effects of p-CA on probiotic properties, as well as in vitro effects on AChE and BChE activities were examined. Results indicated p-CA did not negatively affect bacterial growth, but increased auto-aggregation of both probiotics. While p-CA increased co-aggregation of LA-5 and LGG against Escherichia coli, it decreased co-aggregation against Staphylococcus aureus. Probiotics grown with p-CA were more resistant to pepsin. While p-CA increased the resistance of LA-5 to bile salt, it decreased the resistance of LGG. The combinations of bacteria and p-CA efficiently suppressed AChE and BChE with inhibition (%) 11.31-68.06 and 13.47-65.14, respectively. Furthermore, surface analysis was conducted using FTIR to investigate the interaction of p-coumaric acid with LA-5 and LGG, and changes in cell components on the bacterial surface were analyzed. The results, recorded in range of 4000-600 cm⁻¹ with resolution of 4 cm⁻¹, demonstrated that p-CA significantly affected only the phosphate/CH ratio for both bacteria. These results indicate the addition of p-CA to the probiotic growth may enhance the probiotic properties of bacteria.

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Inorganic polyphosphate from the immunobiotic Lactobacillus rhamnosus CRL1505 prevents inflammatory response in the respiratory tract

Cited by 10 publications

References 57 publications

Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042

Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042

Effect of culture parameters on the heat tolerance and inorganic polyphosphate accumulation by Lacticaseibacillus rhamnosus CRL1505, a multifunctional bacterium

Effects of p-coumaric acid on Probiotic Properties of Lactobacillus acidophilus LA-5 and Lacticaseibacillus rhamnosus GG

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