Inorganic Pyrophosphate

Gawehn, Karlfried

doi:10.1016/b978-0-12-091304-6.50089-6

Cited by 11 publications

(5 citation statements)

References 2 publications

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“…From the entire clinical trial (232 infants), a sample group of 24 infants was randomly chosen for the analysis of D(-)-lactic acid in the blood at 6 months of age (after 6 months of supplementation). Blood was drawn and frozen at 220°C before being shipped on dry ice for analysis of plasma D(-)-lactic acid content at the Centre for Inherited Metabolic Diseases, Karolinska University Hospital, (Stockholm, Sweden), using a validated and regularly used D(-)-lactic acid analytical method based on the method of Gawehn (15). The samples were extracted with perchloric acid before the assay.…”

Section: Methodsmentioning

confidence: 99%

Safety of D(‐)‐Lactic Acid Producing Bacteria in the Human Infant

Connolly

Abrahamsson²,

Björkstén³

2005

J. pediatr. gastroenterol. nutr.

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Section: Methodsmentioning

confidence: 99%

Safety of D(‐)‐Lactic Acid Producing Bacteria in the Human Infant

Connolly

Abrahamsson²,

Björkstén³

2005

J. pediatr. gastroenterol. nutr.

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“…Mannose 6-Phosphate Assay. This coupled enzyme assay is a modification of one described by Gawehn (1974). The sample, containing 4-10 nmol of organic phosphate, was hydrolyzed in 1 N trifluoroacetic acid at 120 °C for 2 h in a sealed glass tube.…”

Section: Methodsmentioning

confidence: 99%

Characterization of phosphorylated oligomannosides from Hansenula wingei mannoprotein

Hashimoto¹,

Cohen²,

Ballou³

1980

Biochemistry

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Yeast cell wall mannoproteins often contain phosphate esterified to the oligosaccharide side chains, and partial acetolysis yields both neutral and phosphorylated fragments [Thieme, T. R., & Ballou, C. E. (1971) Biochemistry 10, 4121--4129]. We have isolated the acidic acetolysis fragments from Hansenula wingei mannoprotein [Yen, P. H., & Ballou, C. E. (1974) Biochemistry 13, 2420--2427] and have separated them into a mannopentaose monophosphate (Man5P) and a mannotriose monophosphate (Man3P). On the basis of selective enzymatic and partial acid hydrolysis and 1H and 31P NMR studies, Man5P was shown to have the structure P leads to 6 alpha Man1 leads to 3 alpha Man1 leads to 2 alpha Man1 leads to 2 alpha Man1 leads to 2Man (where Man = D-mannopyranose). The 31P NMR spectrum of the Man3P showed it to be a mixture of a phosphate monoester and a phosphate diester, which was separated by ion-exchange chromatography. The monoester had the structure P leads to 6 alpha Man1 leads to 3 alpha Man1 leads to 3Man whereas the diester had the properties of a cyclic phosphate. Although native H. wingei mannoprotein contains phosphodiester linkages, the starting mannoprotein preparation was isolated under alkaline conditions that hydrolyze such bonds, and it did not show a phosphodiester signal in the 31P NMR spectrum. We conclude that the cyclic phosphate was an artifact formed during the acetolysis reaction. Because acetolysis of H. wingei mannoprotein yields only phosphorylated mannotriose and mannopentaose whereas the mannoprotein contains mannotetraose side chains as well, the phosphorylation process must be a very specific event in the biosynthesis of the glycoprotein.

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“…The grass silage fermentation characteristics: pH, ammonia fraction (NH 3 -N/total N) (ISO 5983-2, without previous destruction), lactic acid (enzymatic) (Gawehn, 1984), volatile fatty acids and alcohols (Jouany, 1981) were analyzed from an aqueous extract of 100 g grass silage.…”

Section: Sampling and Analyses Of Prewilted Grass And Grass Silagesmentioning

confidence: 99%

Management strategies to improve the nitrogen use efficiency in grasslands and the protein quality in grass silages: Lessons from a practical study on dairy farms in Flanders

Van den Bossche,

De Boever,

Haesaert

et al. 2024

Grassland Research

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BackgroundGrasslands are the primary source of forage for ruminants. Legal restrictions on nitrogen fertilization force farmers to optimize nitrogen use efficiency (NUE) of grassland and protein quality in grass silage.MethodsThe present study included grassland data from 10 dairy farms in Flanders. For each grassland field, the presence of clover was determined based on the seed mixture composition. Soil and manure were sampled before the first application and analyzed to determine plant available N content. The annual NUE was calculated for each field possible (n = 28). Prewilted grass samples were taken just before ensiling (n = 65) and grass silage samples (n = 41) were collected from the same fields.ResultsClover fields demonstrated improved NUE due to a lower nitrogen input from fertilizers compared to pure grassland fields (75 vs. 265 kg ha−1). The protein quality of the grass silage was positively correlated with the organic matter digestibility, sugar content, and dry matter content of the prewilted grass. Conversely, crude ash content had a negative effect.ConclusionsThese findings underscore the importance and potential of clover and the practical feasibility of optimizing grassland management to improve both NUE in grasslands and protein quality of the silage.

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Inorganic Pyrophosphate

Cited by 11 publications

References 2 publications

Safety of D(‐)‐Lactic Acid Producing Bacteria in the Human Infant

Safety of D(‐)‐Lactic Acid Producing Bacteria in the Human Infant

Characterization of phosphorylated oligomannosides from Hansenula wingei mannoprotein

Management strategies to improve the nitrogen use efficiency in grasslands and the protein quality in grass silages: Lessons from a practical study on dairy farms in Flanders

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