Inorganic Selenium Transformation into Organic Selenium by Monascus purpureus

Sun, Nan; Dang, Hui; Zhang, Yuyao; Yang, Mengjie; Zhang, Wei; Zhao, Yu; Zhang, Haisheng; Ji, Hua; Zhang, Baoshan

doi:10.3390/foods12183375

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…The presence of this corona increases the production of biogenic (organic) form of Se. 5 The concentration of inorganic Se plays an important role in the detoxification and biosynthesis of selenoprotein. Only an adequate concentration of inorganic Se is required to initiate the formation of the biogenic form of selenoprotein.…”

Section: Introductionmentioning

confidence: 99%

Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis

Ullah,

Yin,

Naveed

et al. 2024

Biotech and App Biochem

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In this study, the transcriptome analysis was practiced to identify potential genes of probiotic Bacillus subtilis BSN313 involved in selenium (Se) enrichment metabolism. The transcriptomic variation of the strain was deliberated in presence of three different sodium selenite concentrations (0, 3, and 20 μg/mL). The samples were taken at 1 and 13 h subsequent to inoculation of selenite and gene expression profiles in Se metabolism were analyzed through RNA sequencing. The gene expression levels of the pre log phase were lower than the stationary phase. It is because, the bacteria has maximum grown with high concentration of Se (enriched with organic Se), at stationary phase. Bacterial culture containing 3 μg/mL concentration of inorganic Se (sodium selenite) has shown highest gene expression as compared to no or high concentration of Se. This concentration (3 μg/mL) of sodium selenite (as Se) in the medium promoted the upregulation of thioredoxin reductase expression, whereas its higher Se concentration inhibited the formation of selenomethionine (SeMet). The result of 5 L bioreactor fermentation showed that SeMet was also detected in the fermentation supernatant as the growth entered in the late stationary phase and reached up to 857.3 ng/mL. The overall intracellular SeMet enriched content in BSN313 was extended up to 23.4 μg/g dry cell weight. The other two selenoamino acids (Se‐AAs), methyl‐selenocysteine, and selenocysteine were hardly detected in medium supernatant. From this study, it was concluded that SeMet was the highest content of organic Se byproduct biosynthesized by B. subtilis BSN313 strain in Se‐enriched medium during stationary phase. Thus, B. subtilis BSN313 can be considered a commercial probiotic strain that can be used in the food and pharmaceutical industries. This is because it can meet the commercial demand for Se‐AAs (SeMet) in both industries.

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Section: Introductionmentioning

confidence: 99%

Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis

Ullah,

Yin,

Naveed

et al. 2024

Biotech and App Biochem

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Overexpression of Wheat Selenium-Binding Protein Gene TaSBP-A Enhances Plant Growth and Grain Selenium Accumulation under Spraying Sodium Selenite

Xiao,

Qiang,

Sun

et al. 2024

IJMS

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Selenium (Se) is an essential trace element for humans. Low concentrations of Se can promote plant growth and development. Enhancing grain yield and crop Se content is significant, as major food crops generally have low Se content. Studies have shown that Se biofortification can significantly increase Se content in plant tissues. In this study, the genetic transformation of wheat was conducted to evaluate the agronomic traits of non-transgenic control and transgenic wheat before and after Se application. Se content, speciation, and transfer coefficients in wheat grains were detected. Molecular docking simulations and transcriptome data were utilized to explore the effects of selenium-binding protein-A TaSBP-A on wheat growth and grain Se accumulation and transport. The results showed that TaSBP-A gene overexpression significantly increased plant height (by 18.50%), number of spikelets (by 11.74%), and number of grains in a spike (by 35.66%) in wheat. Under normal growth conditions, Se content in transgenic wheat grains did not change significantly, but after applying sodium selenite, Se content in transgenic wheat grains significantly increased. Analysis of Se speciation revealed that organic forms of selenomethionine (SeMet) and selenocysteine (SeCys) predominated in both W48 and transgenic wheat grains. Moreover, TaSBP-A significantly increased the transfer coefficients of Se from solution to roots and from flag leaves to grains. Additionally, it was found that with the increase in TaSBP-A gene overexpression levels in transgenic wheat, the transfer coefficient of Se from flag leaves to grains also increased.

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Inorganic Selenium Transformation into Organic Selenium by Monascus purpureus

Cited by 2 publications

References 40 publications

Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis

Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis

Overexpression of Wheat Selenium-Binding Protein Gene TaSBP-A Enhances Plant Growth and Grain Selenium Accumulation under Spraying Sodium Selenite

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