Inorganic Volumetric Analysis

Rodden, C. J.

doi:10.1021/ac60037a024

Cited by 1 publication

(2 citation statements)

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“…That the precipitation was complete was shown by measurement of the optical density at 260 m~ after removal of the uranyl salt by dialysis. After removal of the precipitate the quantity of uranyl ion remaining in solution was determined by complexing with ferrocyanide (31). The amount of complex formed was determined by measuring the optical density of the solution at 500 m# on either a Beckman DU or Cary Model 11 recording spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

“…Specimens prepared for observation in the electron microscope showed primarily long thin strands with little evidence of denaturation. (31); and (b) titration of dilute solutions with 0.1 N NaOH (31). Repeated tests showed that the deviations in the concentrations of the prepared solutions from the value determined by weighing were always less than 5 per cent, which is about the limit of accuracy of the analytical tests employed.…”

Section: Dnamentioning

confidence: 97%

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Electron Stains

Zobel

Beer

1961

The Journal of Cell Biology

107

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Chemical studies have been carried out on the interaction of DNA with uranyl salts. The effect of variations in pH, salt concentration, and structural integrity of the DNA on the stoichiometry of the salt-substrate complex have been investigated. At pH 3.5 DNA interacts with uranyl ions in low concentration yielding a substrate metal ion complex with a UO++/P mole ratio of about ~ and having a large association constant. At low pH's (about 2.3) the mole ratio decreases to about 1/~. Destruction of the structural integrity of the DNA by heating in HCHO solutions leads to a similar drop in the amount of metal ion bound. Raising the pH above 3.5 leads to an apparent increase in binding as does increasing the concentration of the salt solution. This additional binding has a lower association constant. Under similar conditions DNA binds about seven times more uranyl ion than bovine serum albumin, indicating useful selectivity in staining for electron microscopy.

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Section: Methodsmentioning

confidence: 99%

Section: Dnamentioning

confidence: 97%