Inorganic zinc supplementation modulates heat shock and immune response in heat stressed peripheral blood mononuclear cells of periparturient dairy cows

Sheikh, Aasif Ahmad; Aggarwal, Anjali; Indu, B; Aarif, Ovais

doi:10.1016/j.theriogenology.2017.02.024

Cited by 23 publications

(10 citation statements)

References 55 publications

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“…In the present study, the mRNA expression profile of HSP70 in cultured PBMCs is consistent with previous findings involving broilers [32], sheep [33], goats [34] and humans [35]. Sheikh et al [36] found an increase in HSP70 protein expression in cultured PBMCs when exposed to 42 °C for 3 h of incubation, which is consistent with the results of the present study. In another study, Bhanuprakash et al [6] found an increase in HSP70 concentration after heat shock at 42 °C for 1 h in PBMCs of cows, which is aligned with our present study.…”

Section: The Mrna Expression Of Hsp70 Hsp90 and Hspb1 In Pbmcs Cell supporting

confidence: 93%

Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves

Kim

Lee

Roh

et al. 2020

Animals

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This study was conducted to investigate the effect of HS on HSPs gene expression in bovine PBMCs of beef calves in in vitro and in vivo models. In the in vitro experiment, blood samples were collected from the jugular vein of five beef calves (age: 174.2 ± 5.20 days, BW: 145.2 ± 5.21 kg). In the in vivo experiment, sixteen Korean native male beef calves (age: 169.6 ± 4.60 days, BW: 136.9 ± 6.23 kg) were exposed to ambient temperature for seven days (22 to 24 °C, relative humidity 60%; temperature–humidity index (THI) = 68 to 70) and subsequently to the temperature and humidity corresponding to the target THI level for 21 days (HS). For PBMC isolation, blood samples were collected every three days. In the in vitro model, the cell viability was significantly decreased in HS groups compared with the control group (p = 0.015). The expression of HSP70 (p = 0.022), HSP90 (p = 0.003) and HSPB1 (p = 0.026) genes was increased in the HS group in in vitro model. In the in vivo experiment, the HSP70 gene expression was increased after sudden exposure to HS conditions (severe THI levels; THI = 88 to 90), whereas HSP90 and HSPB1 showed no differences among the THI groups (p > 0.05). However, in the severe THI group, the HSP70 gene expression returned to normal range after six days of continuous HS. In conclusion, the HSP70 gene plays a pivotal role in protecting cells from damage and is sensitive to HS in immune cells compared with other HSP genes in in vitro and in vivo models. In addition, the in vivo models suggest that calves exhibit active physiological mechanisms of adaptation to HS after six days of continuous exposure by regulating the HSP70 gene expression.

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Section: The Mrna Expression Of Hsp70 Hsp90 and Hspb1 In Pbmcs Cell supporting

confidence: 93%

Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves

Kim

Lee

Roh

et al. 2020

Animals

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“…Batistel et al [64] suggested that ethyl-cellulose rumen-protected methionine can increase cattle dry matter intake and increase live weight gain. Furthermore, Sheikh et al [65] reported that inorganic zinc can reduce the concentration of heat shock protein and interleukin 6 (IL-6) in heat-stressed cows, thereby enhancing the body's immunity and alleviating heat stress. Recently, Sun et al [66] found that the addition of zymosan in diet can effectively increase dry matter intake, reduce respiratory rate, and thereby increase milk yield.…”

Section: Discussionmentioning

confidence: 99%

Temporal–Spatial Distribution of Risky Sites for Feeding Cattle in China Based on Temperature/Humidity Index

2020

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This study identifies risk areas for cattle husbandry based on temperature and a relative humidity index (THI) derived from climate data (1987 to 2016) at 839 meteorological stations in China using geostatistics (ordinary and indicator kriging) in the geographical information system (GIS). In general, monthly mean THI values were the highest in July and the lowest in January for all regions. The correlation analysis showed that there were negative relationships between THI values and latitude or elevation for the whole year (p < 0.01). The THI values were higher at low latitudes in coastal areas and at high latitudes in arid areas in summer. The healthy risk for cattle production varied depending on the time of the year and region. The study shows that cattle production is suitable throughout the whole year in the Qinghai-Tibet Plateau; from October to April for most areas, except the southern coastal areas; in May and September in Northeast China, North China, and parts of Northwest China; in June in Heilongjiang and Inner Mongolia. The information obtained in this study can provide a regional distribution of risk for the cattle industry in China.

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“…The anti-inflammatory effect of iHsp70 is reflected in the fact that it inhibits the response to lipopolysaccharides and blocks the production of inflammatory mediators such as TNF-α, and other mechanisms have been described [12]. Gene expression for Hsp70 production is well studied in ruminants or their cell cultures exposed to high ambient temperatures, and multiple increases of iHsp70 in cells provide better adaptation to heat stress [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

The Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant Model

et al. 2021

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Metabolic stress in early lactation cows is characterized by lipolysis, ketogenesis, insulin resistance and inflammation because of negative energy balance and increased use of lipids for energy needs. In this study the relationship between lipid metabolite, lipid-based insulin resistance, and hepatocyte functionality indexes and tumor necrosis factor alpha (TNF-α) with extracellular heat shock protein 70 (eHsp70) was investigated. The experiment included 50 cows and all parameters were measured in blood serum. In cows with a more pronounced negative energy balance, the following was determined: a higher concentration of eHsp70, TNF-α, non-esterified fatty acid (NEFA), beta-hydroxybutyrate (BHB), NEFA to insulin and NEFA to cholesterol ratio and lower concentration of cholesterol, very low-density lipoproteins (VLDL), low density lipoproteins (LDL) and liver functionality index (LFI). The eHsp70 correlated negatively with the values of cholesterol, VLDL, LDL, and triglycerides, while correlated positively with the level of NEFA and BHB. A higher concentration of eHsp70 suggests the development of fatty liver (due to a higher NEFA to cholesterol ratio and lower LFI) and insulin resistance (due to a lower revised quantitative insulin sensitivity check index RQUICKI-BHB and higher NEFA to insulin ratio). The eHsp70 correlated positively with TNF-α. Both TNF-α and eHsp70 correlated similarly to lipid metabolites. In cows with high eHsp70 and TNF-α values we found higher concentrations of NEFA, BHB, NEFA to insulin and NEFA to cholesterol ratio and a lower concentration of triglycerides and VLDL cholesterol compared to cows that had only high TNF-α values. Based on the positive correlation between eHsp70 and TNF-α, their similar relations, and the additional effect of eHsp70 (high TNF-α + eHsp70 values) on lipid metabolites we conclude that eHsp70 has pro-inflammatory effects implicating lipolysis, fatty liver, and fat tissue insulin resistance.

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Inorganic zinc supplementation modulates heat shock and immune response in heat stressed peripheral blood mononuclear cells of periparturient dairy cows

Cited by 23 publications

References 55 publications

Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves

Heat-Shock Proteins Gene Expression in Peripheral Blood Mononuclear Cells as an Indicator of Heat Stress in Beef Calves

Temporal–Spatial Distribution of Risky Sites for Feeding Cattle in China Based on Temperature/Humidity Index

The Correlation between Extracellular Heat Shock Protein 70 and Lipid Metabolism in a Ruminant Model

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