Inositol-1,4,5-trisphosphate accumulation induced by urinary pheromones in female rat vomeronasal epithelium

Sasaki, Kazuyo; Okamoto, Kazuhira; Inamura, Kouhei; Tokumitsu, Yukiko; Kashiwayanagi, Makoto

doi:10.1016/s0006-8993(99)01164-6

Cited by 38 publications

(29 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results suggest that MHC class I peptides stimulate the female VNO via the production of Ins(1,4,5)P 3 . This result complements other studies that show Ins(1,4,5)P 3 levels increase in the VNO membrane preparations during stimulation with urinary pheromones (Sasaki et al, 1999;Kroner et al, 1996;Wekesa and Anholt, 1997;Wekesa et al, 2003;Inamura et al, 1997a;Inamura et al, 1997b). The role of Ins(1,4,5)P 3 in the production of calcium and generation of an action potential is still uncertain along with the role of calcium once it has entered the cell.…”

Section: Discussionsupporting

confidence: 85%

Pregnancy block by MHC class I peptides is mediatedviathe production of inositol 1,4,5-trisphosphate in the mouse vomeronasal organ

Thompson

McMillon

Napier

et al. 2007

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARY The vomeronasal organ (VNO) has evolved to link an animal's behavior to its environment in a highly species-specific fashion. In mice, it is thought to be the primary sensory system responsible for the detection of pheromones. Pheromones regulate a variety of responses including mate recognition in the context of selective pregnancy failure. MHC (major histocompatibility complex)class I peptides have been identified as compounds that elicit the pregnancy block effect via the VNO. However, the transduction cascade of these molecules is unknown and it is not known if the production of these compounds are androgen dependent. By using male urine and MHC peptides, we show that female mice treated with MHC peptides (in urine or PBS) and urine from castrated males or juvenile mice of different haplotypes respond to the Bruce Effect paradigm in a manner equivalent to female mice exposed to whole urine. In addition to providing new evidence that urine from castrated or juvenile males and MHC peptides can induce pregnancy block, we show correlation of the effect with an increase in inositol 1,4,5-trisphosphate.

show abstract

Section: Discussionsupporting

confidence: 85%

Pregnancy block by MHC class I peptides is mediatedviathe production of inositol 1,4,5-trisphosphate in the mouse vomeronasal organ

Thompson

McMillon

Napier

et al. 2007

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…Thus future research will have to evaluate the function of TRPC2 within the context of VNO receptor cells. Because pheromone receptor signalling [33][34][35] activates G i# or G o -type G-proteins in the VNO [36], which in turn activate PLCβ [37], and because cyclic-nucleotide-gated channels were shown to play no obvious role in mammalian VNO signalling [38], the involvement of TRPC2 in the encoding of VNO receptor potentials represents an attractive hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Cloning, expression and subcellular localization of two novel splice variants of mouse transient receptor potential channel 2

Hofmann

Schaefer

Schultz

et al. 2000

Biochemical Journal

View full text Add to dashboard Cite

Transient receptor potential channels (TRPCs) are known as candidate molecular correlates of receptor-activated or store-operated calcium entry. While functional roles for most TRPCs have been suggested, the physiological relevance of TRPC2 remains obscure. Whereas human and bovine TRPC2 are candidate pseudogenes, full-length rodent TRPC2 transcripts have been reported. There is, however, considerable controversy concerning mRNA splicing, tissue distribution and the function of these proteins. We report the molecular cloning of two novel murine TRPC2 splice variants, mTRPC2α and mTRPC2β. mTRPC2α RNA is expressed at low levels in many tissues and cell systems, while mTRPC2β is exclusively and abundantly expressed in the vomeronasal organ (VNO). When expressed in human embryonic kidney (HEK)-293 cells, mTRPC2 did not enhance receptor- or store-activated calcium entry. In order to investigate the basis of such a functional defect, mTRPC2–green fluorescent protein fusion proteins were examined by confocal microscopy. Fusion proteins were retained in endomembranes when expressed in HEK-293 or other cells of epithelial or neuronal origin. Co-expression of TRPC2 with other TRPCs did not restore plasma-membrane trafficking. We conclude that TRPC2 may form functional channels in the cellular context of the VNO, but is unlikely to have a physiological function in other tissues. The sequences of mTRPC2α and mTRPC2β have been submitted to GenBank under the accession numbers AF230802 and AF230803 respectively.

show abstract

“…Pheromones, which are vital for social and sexual intercourse, are released via the urine in adult rats (Sasaki et al 1999). Other than the preputial glands, the source of pheromones in the reproductive organs is unclear.…”

Section: Er Mrna Expression In the Sex Accessory Glandsmentioning

confidence: 99%

Expression of estrogen receptor-alpha and -beta mRNAs in the male reproductive system of the rat as revealed by in situ hybridization

Mowa

Iwanaga²

2001

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

We mapped the cellular expression of estrogen receptor (ER) and ER mRNAs in the male reproductive system of the rat during development and adulthood by in situ hybridization. The expression patterns of ER mRNA in the gonad, efferent duct and initial segment of the epididymis during the perinatal period were essentially similar to those of the adult: ER mRNA signals were expressed most intensely in the epithelia of the efferent ducts and initial segment of the epididymis, and in the interstitial cells of the testis from the prenatal period to adulthood. However, ER mRNA signals in the primordial epididymis and vas deferens during the prenatal period were confined to the outermost cellular layer of the ducts, whereas thereafter they were only expressed weakly in the epithelium and stroma of the epididymis and moderately in the muscle layer of the vas deferens. ER signals were expressed intensely (1) in primordial germ and Sertoli cells only during the prenatal period, (2) in arterial walls in the adult testis, and (3) in the epithelium of the sex accessory glands from the perinatal period to adulthood. This report is the first to describe the cellular distribution of ER mRNA in the male reproductive organs during the perinatal period, and complements and confirms earlier data on its distribution in the adult. The broad expression of ERs in male reproductive organs suggests roles for estrogen in regulating tissue development and reproductive events.

show abstract

Inositol-1,4,5-trisphosphate accumulation induced by urinary pheromones in female rat vomeronasal epithelium

Cited by 38 publications

References 36 publications

Pregnancy block by MHC class I peptides is mediatedviathe production of inositol 1,4,5-trisphosphate in the mouse vomeronasal organ

Pregnancy block by MHC class I peptides is mediatedviathe production of inositol 1,4,5-trisphosphate in the mouse vomeronasal organ

Cloning, expression and subcellular localization of two novel splice variants of mouse transient receptor potential channel 2

Expression of estrogen receptor-alpha and -beta mRNAs in the male reproductive system of the rat as revealed by in situ hybridization

Contact Info

Product

Resources

About