Inositol bis-, tris-, and tetrakis(phosphate)s: analysis in tissues by HPLC.

Meek, James L.

doi:10.1073/pnas.83.12.4162

Cited by 85 publications

(31 citation statements)

References 13 publications

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“…The excess trichloroacetic acid was removed from the supernatant containing the soluble phosphorylated inositols by extracting the solution five times with 2 ml diethylether. Subsequently, the samples were analysed for inositol phosphates by FPLC on a Mono Q @ HR 5/5 anion exchange column according to (Meek, 1986). As an internal standard 10 pM ATP, ADP and AMP were added to each extract.…”

Section: Real-time Binding Experiments Using the Biacoretmmentioning

confidence: 99%

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Tenascin: a modulator of cell growth

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Panayotou

Entwistle

et al. 1992

European Journal of Biochemistry

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The large, multidomain extracellular matrix protein tenascin displays a markedly restricted tissue distribution during embryogenesis and remains present only in a few adult tissues. The protein is re‐expressed, however, during wound healing and in the stroma of malignant tumours. While a variety of studies have dealt with the important role of tenascin in the development of neural and non‐neural tissues, there is growing evidence that tenascin expression may be associated with proliferation of cells lining these tissues. The presence of repeating domains in tenascin similar to those in epidermal growth factor prompted us to investigate the ability of tenascin to modulate the growth of different cell types. Tenascin was actually found to be mitogenic for several cell types. This mitogenic activity, however, appears to be associated with a region in the fibronectin type III domains. The mitogenic mechanism is clearly distinct from pathways used by peptide growth factors such as epidermal growth factor and platelet‐derived growth factor, which activate the intrinsic tyrosine kinase activity of their cell‐surface receptors. However, we show that this large extracellular matrix molecule is efficiently internalised and may be processed by responding cells.

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Section: Real-time Binding Experiments Using the Biacoretmmentioning

confidence: 99%

“…3 ) have been raised against the intact hexameric form and fragments of (Meek, 1986 chick tcnascin. The epitopes werc determined in studies using electron microscopy as well as Western blotting to proteolytic fragments .…”

Section: Internalisation Of Tenascinmentioning

confidence: 99%

Tenascin: a modulator of cell growth

End

Panayotou

Entwistle

et al. 1992

European Journal of Biochemistry

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“…Irradiation of the whole rat head with the focused microwave for a few seconds kills the rat instantly, and the postmortem changes in the cyclic nucleotides, several neurotransmitters, and intermediary glucose metabolites in the brain can be significantly reduced (13). The focused microwave technique can also deactivate cellular enzymes involved in the phosphoinositide cascade in the brain and the salivary gland (19). However, because of the high energy required (kW for the rat head), the focused microwave device has not been expanded to accommodate a big subject such as the rabbit.…”

Section: Discussionmentioning

confidence: 99%

In vivocAMP level in rabbit iris-ciliary body after topical epinephrine treatment

Liu

Gallar

1996

Current Eye Research

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“…It is now accepted that inositoltriphosphate is an intracellular mediator of cytoplasmic calcium, yet the concentration of inositoltriphosphate used to document that fact in saponin-permeabolized hepatocytes is 1 /^M (13). This is pharmacological compared to endogenous levels of inositoltriphosphate in liver (13 nmol/g liver wt) (28).…”

Section: Discussionmentioning

confidence: 99%

Lysophosphatidylinositol: A Potential Mediator of 1,25-Dihydroxyvitamin D-Induced Increments in Hepatocyte Cytosolic Calcium*

Baran

Kelly²

1988

Endocrinology

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Recent studies in our laboratory demonstrate that 1,25-dihydroxyvitamin D [1,25-(OH)2D] increases hepatocyte cytosolic calcium in the absence of extracellular calcium and activates phospholipase-A-induced deacylation of phosphatidylinositol within 5 min. To determine whether inhibition of phospholipase-A affects D-induced increments in cytosolic calcium and which metabolite of phosphatidylinositol mediates these increments, cytosolic calcium is measured in cultured hepatocytes, loaded with Fura 2AM. Cellular fluorescence is determined at excitation wavelengths of 340 and 380 nm, and cytosolic calcium is calculated. 1,25-(OH)2D treatment for 5 min increases cytosolic calcium levels in the cultured hepatocyte. Inhibition of phospholipase-A with bromophenacylbromide blocks the vitamin D effect on cytosolic calcium, indicating that phospholipase-A activation precedes increments in cytosolic calcium. Neither arachidonic acid nor sodium arachidonate affects cytosolic calcium. In contrast, LPI increases hepatocyte cytosolic calcium in the presence and absence of extracellular calcium. The effect is not blocked by bromophenacylbromide. Neither cell viability nor supernatant fluorescence is altered by LPI, indicating that LPI is increasing fluorescence within the cell. 1,25-(OH)2D treatment for 5 min increases cytosolic pH in cultured hepatocytes. Inhibition of cell alkalinization with amiloride blocks the vitamin D effect on both cytosolic pH and cytosolic calcium, but not the effect of LPI on calcium. NH4Cl increases hepatocyte pH but not cytosolic calcium. The results indicate that phospholipase-A activation is necessary for 1,25-(OH)2D-induced increments in hepatocyte cytosolic calcium and that LPI, a deacylation product of phosphatidylinositol, mediates these increments. Furthermore, inhibition of Na+/H+ exchange, while not blocking the ability of the cell to increase cytosolic calcium in response to LPI, prevents the 1,25-(OH)2D-induced increments in both pH and calcium. The data suggest that the rapid effects of 1,25-(OH)2D on hepatocyte calcium are preceded by cell alkalinization and phospholipase-A activation, and these effects appear to be linked to the Na+/H+ antiport system and not nonspecific cell alkalinization.

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Inositol bis-, tris-, and tetrakis(phosphate)s: analysis in tissues by HPLC.

Cited by 85 publications

References 13 publications

Tenascin: a modulator of cell growth

Tenascin: a modulator of cell growth

In vivocAMP level in rabbit iris-ciliary body after topical epinephrine treatment

Lysophosphatidylinositol: A Potential Mediator of 1,25-Dihydroxyvitamin D-Induced Increments in Hepatocyte Cytosolic Calcium*

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