Inositol Monophosphatase: A Bifunctional Enzyme in <i>Mycobacterium smegmatis</i>

Goswami, Rajendra; Bondoc, Jasper Marc G.; Wheeler, Paul R.; Jafari, Alireza; González, Trinidad González; Mehboob, Shahila; Movahedzadeh, Farahnaz

doi:10.1021/acsomega.8b01753

Cited by 6 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Urea carboxylase is involved in arginine biosynthesis, atrazine degradation, and metabolic pathways ( 38 , 39 ). Myo -Inositol-1 (or -4)-monophosphatase is involved in streptomycin biosynthesis, inositol phosphate metabolism, metabolic pathways, the biosynthesis of secondary metabolites, and the phosphatidylinositol signaling system ( 40 – 43 ). Since these genes are not closely related to other significant genes and are involved in many different pathways, further research would be required to understand the role that they play in bacterial persistence.…”

Section: Discussionmentioning

confidence: 99%

Flagellar Genes Are Associated with the Colonization Persistence Phenotype of the Drosophila melanogaster Microbiota

Morgan

Chaston

2023

Microbiol Spectr

View full text Add to dashboard Cite

Despite the growing body of research on the microbiota, the mechanisms by which the microbiota colonizes a host can still be further elucidated. This study identifies bacterial genes that are associated with the colonization persistence phenotype of the microbiota in Drosophila melanogaster , which reveals specific bacterial factors that influence the establishment of the microbiota within its host.

show abstract

Section: Discussionmentioning

confidence: 99%

Flagellar Genes Are Associated with the Colonization Persistence Phenotype of the Drosophila melanogaster Microbiota

Morgan

Chaston

2023

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…Inositol has important biological functions in plants, which not only participates in the formation of cell walls and cell membranes, but also plays an important role in various life activities such as osmotic regulation, signal transduction, and stress regulation ( Zhang et al, 2013 ; Johnen, 2021 ). Inositol monophosphatase (IMPase) is a catalytic enzyme for the final step of inositol biosynthesis ( Goswami et al, 2018 ). Studies have shown that abiotic stresses such as low temperature, drought and high salinity can affect the activity of IMPase ( Nourbakhsh et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Physiological and Proteomic Responses of Contrasting Alfalfa (Medicago sativa L.) Varieties to High Temperature Stress

Li¹,

Zhang³

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

High temperature (HT) is an important factor for limiting global plant distribution and agricultural production. As the global temperature continues to rise, it is essential to clarify the physiological and molecular mechanisms of alfalfa responding the high temperature, which will contribute to the improvement of heat resistance in leguminous crops. In this study, the physiological and proteomic responses of two alfalfa (Medicago sativa L.) varieties contrasting in heat tolerance, MS30 (heat-tolerant) and MS37 (heat-sensitive), were comparatively analyzed under the treatments of continuously rising temperatures for 42 days. The results showed that under the HT stress, the chlorophyll content and the chlorophyll fluorescence parameter (Fv/Fm) of alfalfa were significant reduced and some key photosynthesis-related proteins showed a down-regulated trend. Moreover, the content of Malondialdehyde (MDA) and the electrolyte leakage (EL) of alfalfa showed an upward trend, which indicates both alfalfa varieties were damaged under HT stress. However, because the antioxidation-reduction and osmotic adjustment ability of MS30 were significantly stronger than MS37, the damage degree of the photosynthetic system and membrane system of MS30 is significantly lower than that of MS37. On this basis, the global proteomics analysis was undertaken by tandem mass tags (TMT) technique, a total of 6,704 proteins were identified and quantified. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that a series of key pathways including photosynthesis, metabolism, adjustment and repair were affected by HT stress. Through analyzing Venn diagrams of two alfalfa varieties, 160 and 213 differentially expressed proteins (DEPs) that had dynamic changes under HT stress were identified from MS30 and MS37, respectively. Among these DEPs, we screened out some key DEPs, such as ATP-dependent zinc metalloprotease FTSH protein, vitamin K epoxide reductase family protein, ClpB3, etc., which plays important functions in response to HT stress. In conclusion, the stronger heat-tolerance of MS30 was attributed to its higher adjustment and repair ability, which could cause the metabolic process of MS30 is more conducive to maintaining its survival and growth than MS37, especially at the later period of HT stress. This study provides a useful catalog of the Medicago sativa L. proteomes with the insight into its future genetic improvement of heat-resistance.

show abstract

“…Glycosylphosphatidylinositol metabolism was involved in heat tolerance in S. cerevisiae (Nasution et al, 2015). Inositol monophosphatase (MAC_04535) played a key role in the phosphatidylinositol signaling pathway (Goswami et al, 2018). Up-regulation of inositol monophosphatase might contribute to the increased heat tolerance in MaPepDA null mutant.…”

Section: Discussionmentioning

confidence: 99%

Disruption of a C69-Family Cysteine Dipeptidase Gene Enhances Heat Shock and UV-B Tolerances in Metarhizium acridum

Guo

Cao

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

In fungi, peptidases play a crucial role in adaptability. At present, the roles of peptidases in ultraviolet (UV) and thermal tolerance are still unclear. In this study, a C69-family cysteine dipeptidase of the entomopathogenic fungus Metarhizium acridum, MaPepDA, was expressed in Escherichia coli. The purified enzyme had a molecular mass of 56-kDa, and displayed a high activity to dipeptide substrate with an optimal Ala-Gln hydrolytic activity at about pH 6.0 and 55 • C. It was demonstrated that MaPepDA is an intracellular dipeptidase localized in the cytosol, and that it is expressed during the whole fungal growth. Disruption of the MaPepDA gene increased conidial germination, growth rate, and significantly improved the tolerance to UV-B and heat stress in M. acridum. However, virulence and conidia production was largely unaffected in the MaPepDA mutant. Digital gene expression data revealed that the MaPepDA mutant had a higher UV-B and heat-shock tolerance compared to wild type by regulating transcription of sets of genes involved in cell surface component, cell growth, DNA repair, amino acid metabolism, sugar metabolism and some important signaling pathways of stimulation. Our results suggested that disruption of the MaPepDA could potentially improve the performance of fungal pesticides in the field application with no adverse effect on virulence and conidiation.

show abstract

Inositol Monophosphatase: A Bifunctional Enzyme in Mycobacterium smegmatis

Cited by 6 publications

References 24 publications

Flagellar Genes Are Associated with the Colonization Persistence Phenotype of the Drosophila melanogaster Microbiota

Flagellar Genes Are Associated with the Colonization Persistence Phenotype of the Drosophila melanogaster Microbiota

Physiological and Proteomic Responses of Contrasting Alfalfa (Medicago sativa L.) Varieties to High Temperature Stress

Disruption of a C69-Family Cysteine Dipeptidase Gene Enhances Heat Shock and UV-B Tolerances in Metarhizium acridum

Contact Info

Product

Resources

About