Inositol Polyphosphates

Resnick, Adam; Saiardi, Adolfo

doi:10.1002/9780470048672.wecb252

Cited by 6 publications

(7 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, OD was normalized, and the initial value at time 0 was subtracted from all of the following values. Second, to account for a nonlinear correlation between OD and cell density at high cell densities, OD values were calibrated according to the following formula: Calibrated OD ϭ OD ϩ 0.8324 ϫ OD 3 . Third, growth curves were smoothed to reduce contributions from noise by averaging (nonweighted averaging) over three consecutive measurements (no averaging of initial value).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Arginine Transcriptional Response Does Not Require Inositol Phosphate Synthesis

Bosch

Saiardi

2012

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Background:The involvement of inositol phosphates produced by the yeast inositol-polyphosphate multikinase, Arg82, in transcription is controversial. Results: Catalytically inactive Arg82 restores the regulation of arginine-dependent genes in an ARG82 knock-out. Conclusion: Inositol phosphates do not regulate arginine-dependent gene expression. Significance: Independently of its enzymatic activity Arg82 controls arginine-responsive genes.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Inositol phosphates constitute a family of soluble molecules which play a central role in cell signaling (1)(2)(3). The large variety of inositol phosphate species stems from the attachment of one or more phosphate groups to the six carbon myo-inositol ring.…”

mentioning

confidence: 99%

Arginine Transcriptional Response Does Not Require Inositol Phosphate Synthesis

Bosch

Saiardi

2012

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…S. cerevisiae exclusively uses the lipid route to synthesize inositol phosphates [119], whereas D. discoideum utilizes the cytosolic route to synthesize IP 6 [15], the enzymology of which is not fully elucidated. The interested reader is invited to read the reviews [11,120,121] which describe the synthesis of IP 6 in more detail. This Figure highlights the metabolic steps most relevant in vivo, supported by yeast genetic studies [25,26,40,53,119].…”

Section: Figure 1 Linear Pathway Of Inositol Pyrophosphates Synthesismentioning

confidence: 99%

Inositol pyrophosphates: between signalling and metabolism

Wilson

Livermore

Saiardi

2013

Biochemical Journal

Self Cite

233

266

View full text Add to dashboard Cite

The present review will explore the insights gained into inositol pyrophosphates in the 20 years since their discovery in 1993. These molecules are defined by the presence of the characteristic 'high energy' pyrophosphate moiety and can be found ubiquitously in eukaryotic cells. The enzymes that synthesize them are similarly well distributed and can be found encoded in any eukaryote genome. Rapid progress has been made in characterizing inositol pyrophosphate metabolism and they have been linked to a surprisingly diverse range of cellular functions. Two decades of work is now beginning to present a view of inositol pyrophosphates as fundamental, conserved and highly important agents in the regulation of cellular homoeostasis. In particular it is emerging that energy metabolism, and thus ATP production, is closely regulated by these molecules. Much of the early work on these molecules was performed in the yeast Saccharomyces cerevisiae and the social amoeba Dictyostelium discoideum, but the development of mouse knockouts for IP6K1 and IP6K2 [IP6K is IP6 (inositol hexakisphosphate) kinase] in the last 5 years has provided very welcome tools to better understand the physiological roles of inositol pyrophosphates. Another recent innovation has been the use of gel electrophoresis to detect and purify inositol pyrophosphates. Despite the advances that have been made, many aspects of inositol pyrophosphate biology remain far from clear. By evaluating the literature, the present review hopes to promote further research in this absorbing area of biology.

show abstract

“…As it is not the main scope of this essay, discussion of inositol polyphosphate metabolism will be highly simplified; the interested person is encouraged to read the following more comprehensive reviews [15][16][17][18]. The carbon backbone of myoinositol (hereafter simply called ''inositol'') is by far the most common and biologically relevant of the naturally occurring stereoisomers.…”

Section: Phosphoinositides and Inositol Phosphatesmentioning

confidence: 99%

Importance of Radioactive Labelling to Elucidate Inositol Polyphosphate Signalling

Wilson¹,

Saiardi²

2017

Phosphate Labeling and Sensing in Chemical Biology

Self Cite

View full text Add to dashboard Cite

Inositol polyphosphates, in their water-soluble or lipid-bound forms, represent a large and multifaceted family of signalling molecules. Some inositol polyphosphates are well recognised as defining important signal transduction pathways, as in the case of the calcium release factor Ins(1,4,5)P 3 , generated by receptor activation-induced hydrolysis of the lipid PtdIns(4,5)P 2 by phospholipase C. The birth of inositol polyphosphate research would not have occurred without the use of radioactive phosphate tracers that enabled the discovery of the ''PI response''. Radioactive labels, mainly of phosphorus but also carbon and hydrogen (tritium), have been instrumental in the development of this research field and the establishment of the inositol polyphosphates as one of the most important networks of regulatory molecules present in eukaryotic cells. Advancements in microscopy and mass spectrometry and the development of colorimetric assays have facilitated inositol polyphosphate research, but have not eliminated the need for radioactive experimental approaches. In fact, such experiments have become easier with the cloning of the inositol polyphosphate kinases, enabling the systematic labelling of specific positions of the inositol ring with radioactive phosphate. This approach has been valuable for elucidating their metabolic pathways and identifying specific and novel functions for inositol polyphosphates. For example, the synthesis of radiolabelled inositol pyrophosphates has allowed the discovery of a new protein posttranslational modification. Therefore, radioactive tracers have played and will continue to play an important role in dissecting the many complex aspects of

show abstract

Inositol Polyphosphates

Cited by 6 publications

References 67 publications

Arginine Transcriptional Response Does Not Require Inositol Phosphate Synthesis

Arginine Transcriptional Response Does Not Require Inositol Phosphate Synthesis

Inositol pyrophosphates: between signalling and metabolism

Importance of Radioactive Labelling to Elucidate Inositol Polyphosphate Signalling

Contact Info

Product

Resources

About