Inotropic effects of ethanol and dihydropyridines on the Guinea pig heart artrial muscle

Salvatici, R; Gallardo-Carpentier, Adriana; Isaacson, Robert L.; Carpentier, Robert

doi:10.1016/0024-3205(90)90345-r

Life Sciences

1990

DOI: 10.1016/0024-3205(90)90345-r

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Inotropic effects of ethanol and dihydropyridines on the Guinea pig heart artrial muscle

R Salvatici

Adriana Gallardo-Carpentier

Robert L. Isaacson

et al.

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Cited by 8 publications

References 19 publications

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Investigation of calcium antagonist–L-type calcium channel interactions by a vascular smooth muscle cell membrane chromatography method

Wang

et al. 2010

Anal Bioanal Chem

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The dissociation equilibrium constant (K(D)) is an important affinity parameter for studying drug-receptor interactions. A vascular smooth muscle (VSM) cell membrane chromatography (CMC) method was developed for determination of the K(D) values for calcium antagonist-L-type calcium channel (L-CC) interactions. VSM cells, by means of primary culture with rat thoracic aortas, were used for preparation of the cell membrane stationary phase in the VSM/CMC model. All measurements were performed with spectrophotometric detection (237 nm) at 37 degrees C. The K(D) values obtained using frontal analysis were 3.36 x 10(-6) M for nifedipine, 1.34 x 10(-6) M for nimodipine, 6.83 x 10(-7) M for nitrendipine, 1.23 x 10(-7) M for nicardipine, 1.09 x 10(-7) M for amlodipine, and 8.51 x 10(-8) M for verapamil. This affinity rank order obtained from the VSM/CMC method had a strong positive correlation with that obtained from radioligand binding assay. The location of the binding region was examined by displacement experiments using nitrendipine as a mobile-phase additive. It was found that verapamil occupied a class of binding sites on L-CCs different from those occupied by nitrendipine. In addition, nicardipine, amlodipine, and nitrendipine had direct competition at a single common binding site. The studies showed that CMC can be applied to the investigation of drug-receptor interactions.

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Investigation of calcium antagonist–L-type calcium channel interactions by a vascular smooth muscle cell membrane chromatography method

Wang

et al. 2010

Anal Bioanal Chem

View full text Add to dashboard Cite

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Mechanisms of the inotropic actions of MPTP and MPP+ on isolated atria of rat

Wilson

Shearer

Adelakun

et al. 1991

Toxicology and Applied Pharmacology

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Cardiac beta-adrenergic mediated chrono- and inotropic effects of imipramine in vitro

Salvatici

Standen

Shulz

et al. 1992

International Journal of Cardiology

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Inotropic effects of ethanol and dihydropyridines on the Guinea pig heart artrial muscle

Cited by 8 publications

References 19 publications

Investigation of calcium antagonist–L-type calcium channel interactions by a vascular smooth muscle cell membrane chromatography method

Investigation of calcium antagonist–L-type calcium channel interactions by a vascular smooth muscle cell membrane chromatography method

Mechanisms of the inotropic actions of MPTP and MPP+ on isolated atria of rat

Cardiac beta-adrenergic mediated chrono- and inotropic effects of imipramine in vitro

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