This study aimed to explore the effect of acupuncture on account of composite diamond-like nanomembrane sensors (DLNF) sensors on post-stroke depression. Titanium/antigen-diamond-like carbon (Ti/Ag-DLC) composite DLNF sensors were prepared by coating Ti and Ag composites on the surface of DLC by radio frequency magnetron sputtering technology. The scanning electron microscope (SEM), X-ray photoelectron spectrometer (XPS), Raman spectrometer, nanoindenter, and sliding friction tester were adopted to analyze the characterization of Ti/Ag-DLC composite membrane, the chemical state of the main constituent elements on the surface, structural characteristics, membrane hardness, and tribological properties, respectively. 132 patients with post-stroke depression in our hospital were selected as the research objects, they were divided into the control group (receiving conventional treatment) and the acupuncture group (receiving acupuncture based on conventional treatment) according to different treatment methods, with 66 cases in each group. The depression of the two groups of patients before the treatment and 4 and 8 weeks after the treatment were compared. The psychological resilience scale and Herth scale were used to evaluate the mental toughness and hope level of patients. The results showed that when the Ag content was less than 0.55%, the Ti/Ag-DLC composite surface was smooth and showed no obvious particles, the membranes with different content had obvious absorption peaks at 1560 cm -1 and the friction life in a high vacuum environment was extended to around 900 revolutions. After 4 and 8 weeks of treatment, the scores of the hammer depression scale (HDS) in the two groups were significantly lower than before treatment (P<0.01) and the scores of the acupuncture group were much lower after 4 and 8 weeks of treatment in contrast to the control group (P<0.01). The optimism, self-strength, and tenacity in the psychological resilience scale of the acupuncture group patients rose higher than those in the control group (P<0.05) after 8 weeks of treatment. The Herth scale score of the acupuncture group was greatly higher compared with that of the control group after 8 weeks of treatment (P<0.01). It showed that the Ti/Ag-DLC composite DLNF sensor with good hardness and friction performance was prepared in this study. Acupuncture therapy could improve the negative emotions of patients with post-stroke depression, enhance mental resilience, and help raise the level of hope. Therefore, it was proved to be a potentially effective treatment.