Insect Hormones and the Regulation of Genetic Activity

Richards, Gareth; Ashburner, Michael

doi:10.1007/978-1-4757-4619-8_6

Cited by 10 publications

(5 citation statements)

References 158 publications

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“…The discovery of early and late transcriptional responses in midge salivary glands after administration of ecdysone led to the proposal in those papers that some of the steroid-responsive early genes code for regulatory (e.g. transcription) factors which are required for the transcription of the late genes (45,46). Such a cascade model might account for the biphasic transcriptional response to steroids observed in higher organisms mentioned earlier in this paper (1,14,18).…”

Section: Dexamethasone Regulation Of C-myc Mrna Levels In Estrogen-wimentioning

confidence: 89%

“…Support for such a model is found in the midge fly system as outlined elsewhere (1,43,44). The steroid ecdysone induces the synthesis of early proteins which localize in the nucleus at the chromosomal domains of late genes (45,46). The discovery of early and late transcriptional responses in midge salivary glands after administration of ecdysone led to the proposal in those papers that some of the steroid-responsive early genes code for regulatory (e.g.…”

Section: Dexamethasone Regulation Of C-myc Mrna Levels In Estrogen-wimentioning

confidence: 94%

“…Such a model could be used to address some of the major questions relating to the mechanisms of steroid hormone action such as: 1) the events occurring right after the rapid association of steroid-activated receptors with specific acceptor sites on oviduct chromatin (1,45,46,61,62), 2) the role of the steroid response elements flanking steroid-responsive genes in binding transcription factors and other regulatory gene products (1,43,44,(52)(53)(54)(55) as opposed to steroid receptors (63)(64)(65)(66)(67), and 3) the role, if any, of changes in chromatin structure and/or binding of transcription factors to the gene's promoter region induced by steroids (47-53, 68, 69). The 5-to 10-min response of c-myc gene expression to glucocorticoids and progestins suggests that the steroid-activated receptors might interact directly with regulatory sites controlling c-myc mRNA transcription, or if the rapid action of DEX is at the level of the half-life of c-myc mRNA, then a whole new model for steroid action might have to be considered.…”

Section: Dexamethasone Regulation Of C-myc Mrna Levels In Estrogen-wimentioning

confidence: 99%

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Rapid Inhibition of c-mycGene Expression by a Glucocorticoid in the Avian Oviduct

Rories¹,

Lau²,

Fink³

et al. 1989

Molecular Endocrinology

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The glucocorticoid dexamethasone (DEX) causes a rapid, reversible reduction in c-myc mRNA level in the oviducts of estrogen-treated, immature chickens. The c-myc mRNA level begins to decrease by 5 min after injection of 0.5 mg DEX, reaches a minimum of 10% of the control value by 30 min, and returns to 30-40% of the control value by 4 h post injection. This rapid effect of DEX on the c-myc mRNA level occurs in both diethylstilbestrol-stimulated and diethylstilbestrol-withdrawn oviducts. The effect is dose dependent, with reduction of the c-myc mRNA measured with as little as 10 micrograms DEX injection (0.03 micrograms/g BW). The effect of the steroid is gene specific with H2B histone mRNA displaying a significantly reduced response. The effect is also tissue specific with liver displaying an increase of 170% of control values in c-myc mRNA level by 30 min after injection of 0.5 mg DEX. The reduction of avian oviduct c-myc mRNA levels by DEX may play a role in glucocorticoid inhibition of cell proliferation in this tissue. The rapidity of the steroid effects on c-myc expression makes it likely that the steroid-induced reduction of c-myc mRNA levels represents a direct primary action of the steroid-receptor complex on the c-myc gene expression.

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Section: Dexamethasone Regulation Of C-myc Mrna Levels In Estrogen-wimentioning

confidence: 89%

Section: Dexamethasone Regulation Of C-myc Mrna Levels In Estrogen-wimentioning

confidence: 94%

Section: Dexamethasone Regulation Of C-myc Mrna Levels In Estrogen-wimentioning

confidence: 99%

See 1 more Smart Citation

Rapid Inhibition of c-mycGene Expression by a Glucocorticoid in the Avian Oviduct

Rories¹,

Lau²,

Fink³

et al. 1989

Molecular Endocrinology

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“…Polytene chromosomes of dipteran larval and pupal salivary glands undergo a series of morphological changes in response to ecdysteroids; chromosomal "puffs" fOmi and disappear at discrete loci in a temporal progression that eventually involves some 200 genetic units (see 159). Metabolic labeling studies confirmed that most sites of puffing correspond to regions of transcrip tional activity (17), and it has been suggested from immunocytochemical data that ecdysteroid receptors localize preferentially at the puff sites (80) .…”

Section: Y Tological and Nuclease Probesmentioning

confidence: 95%

“…In certain invertebrates, notably insects (159) and the water mold Achlya (190), hormonal roles fo r steroids are firmly established. In certain invertebrates, notably insects (159) and the water mold Achlya (190), hormonal roles fo r steroids are firmly established.…”

Section: Origins Of Conserved Gene Regula Torsmentioning

confidence: 99%

Steroid Receptor Regulated Transcription of Specific Genes and Gene Networks

1985

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Larval fat body‐specific gene expression in D. melanogaster

et al. 1989

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The Pl gene, together with the LSP-1 alpha, -1 beta, and -1 gamma, LSP-2, and P6 genes, is expressed exclusively in the larval fat body of D. melanogaster during the third instar. In vivo mapping of the cis-acting regulatory sequences of the P1 gene was carried out using hybrid constructs with three different reporter genes and a combination of transient and germline transformation assays. This revealed that regulatory elements involved in the setting up of the temporal and spatial specificities of transcription of the P1 gene are located in a short DNA region immediately upstream of the mRNA transcription start. This region includes an element that behaves as a fat-body transcriptional enhancer and element(s) required for ecdysone inducibility of transcription of the P1 gene.

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Insect Hormones and the Regulation of Genetic Activity

Cited by 10 publications

References 158 publications

Rapid Inhibition of c-mycGene Expression by a Glucocorticoid in the Avian Oviduct

Rapid Inhibition of c-mycGene Expression by a Glucocorticoid in the Avian Oviduct

Steroid Receptor Regulated Transcription of Specific Genes and Gene Networks

Larval fat body‐specific gene expression in D. melanogaster

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